For example we can visualize
fluorescently labeled proteins and compare the quantity and localization relative to other labeled cellular components.
Both PALM and stimulated emission depletion (STED) microscopy, the other super-resolution technique recognized with the 2014 Nobel Prize, illuminate samples with so much light that
fluorescently labeled proteins fade and the sample is quickly damaged, making prolonged imaging impossible.
Not exact matches
They added
fluorescently labeled ovalbumin (FITC - OVA) to the patches to determine the extent of
protein translocation after NIR irradiation on murine skin.
The researchers were able to visualize the process because the
protein aggregates were
labeled with different
fluorescently tagged
proteins that bind to aggregates.
Quantitative single cell monitoring of
protein synthesis at subcellular resolution using
fluorescently labeled tRNA.
The methodology has been applied to the study of lactose repressor
protein (LacI),
fluorescently labeled with Atto532, and detected as it binds to a DNA molecule (trapped between two optical tweezers) containing specific LacI binding sequences (i.e., operators).
Using the HuProt microarray array, the binding of
fluorescently labeled or biotinylated sample
proteins to
proteins on the HuProt human proteome microarray — the largest collection of human
proteins in the world — can be analyzed simultaneously.
The microarray allows the specificity of
fluorescently labeled, biotinylated or radiolabeled molecules to be assayed against the largest collection of human
proteins in the world.
Flow cytometry is a powerful technique that allows researchers to examine multiple
proteins on cell populations using
fluorescently labeled antibodies.
HuProt Cross-Section: microarray probed with a serum Ig sample and
fluorescently -
labeled secondary antibodies to the Ig and common epitopes of the arrayed
proteins.