Sentences with phrase «fluorescently labelled secondary antibody»
Immunoreactions were developed using a fluorescently labeled secondary antibody, and samples visualized in a Leica Biosystems epifluorescent microscope or using a secondary antibody linked to horseradish peroxidase and developed with a 3,3 ′ - diaminobenzidine kit.
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Immunofluorescent labeling was performed using anti-Olig2 (1 ∶ 4000, Chemicon) and anti-GFAP (1 ∶ 400, Cell Signaling) followed by fluorescently labeled, secondary anti-Ig antibodies (Alexa 488 and 568 conjugates, Invitrogen) at a 1 ∶ 2000 dilution.
HuProt Cross-Section: microarray probed with a serum Ig sample and fluorescently - labeled secondary antibodies to the Ig and common epitopes of the arrayed proteins.