Fluorescing proteins accurately predict the demise of nematode worms, by measuring the activity of free radicals within their cells
Not exact matches
So, the researchers — headed by Professor of Physics and Biological Sciences Shane Hutson and Associate Professor of Cell and Developmental Biology Andrea Page - McCaw — targeted cells on the back of fruit fly pupae that expressed a
protein that
fluoresces in the presence of calcium ions.
The researchers tinkered with the fish's DNA so that a
protein present only in neurons would
fluoresce when the neurons were firing.
Proteins from jellyfish and corals that
fluoresce in the visible wavelength range have revolutionized optical imaging of cells.
The root
fluoresces green when the TCSn gene associated with cytokinin activation is turned on because it is fused with a jellyfish
protein that acts as a reporter signal.
When the drug was absent from a cell, the
proteins fluoresced in a certain color, and when the drug was present, the color changed.
For example, the shells could be functionalized to
fluoresce in the presence of specific
proteins or to target tumors.
The second congregate is made up of two parts: YFP, a marker
protein that
fluoresces yellow under light and CRY2, an optogenetically enhanced
protein activated by light that attracts
protein CIB1 as well as other CRY2.
The first congregate consists of 3 components: CIB1, an attractor that grabs onto CRY2; RFP, a marker that
fluoresces red under light, and Rab GTPase, a
protein that binds to the vesicle membranes within the cell.
* She combined a marker — a gene for a jellyfish
protein that
fluoresces green — with a DNA sequence that turns on the green fluorescent
protein in the presence of thyroid hormones.
TRAP includes a small «biarsenical» probe, which
fluoresces so the team can find the
proteins to which it has become attached.
Some of the more popular timers (whether as part of
protein fusions or expressed independently) originally
fluoresce green or blue and, when they mature,
fluoresce red.
modified the
protein to make it a green
fluorescing monomer, making it a possible stand - in for GFP that could be used with the same microscope filters already used for GFP.
Tight clumps of green
fluorescing FUS form in the untreated yeast cell on the left, but the
protein remains diffuse and unclumped in the phosphorylated cell on the right.
Recently, Shaner et al. modified the
protein to make it a green
fluorescing monomer, making it a possible stand - in for GFP that could be used with the same microscope filters already used for GFP.