Sentences with phrase «fluorophore in»

- Based on the expression level / pattern of the protein of interest, decide what antigen to detect with which fluorophore in multi-color experiments.

A LSFM uses two independent, azimuthally arranged optical paths to excite the fluorophores in a specimen and to detect the fluorescence emission.

In order to specifically label molecules with fluorophores in intact cells, we prepare highly stable unnatural amino acids that rapidly and irreversibly undergo cycloaddition reactions (click chemistry) with unsurpassed speed and study their application in collaboration with the Lemke group.

Not exact matches

In their latest work, Still's team strung these molecules, called chemosensors, with a molecule called a fluorophore that glows when the sensor alters its shape after binding to a peptide.

Recombinant proteins containing tetracysteine tags can be successively labeled in living cells with different colors of biarsenical fluorophores so that older and younger protein molecules can be sharply distinguished by both fluorescence and electron microscopy.

Instead, they create a library of several hundred fluorophores with varying chemical additions and three - dimensional conformations and then test each in mice to see which are taken up by specific tissues in the body.

«We basically deconstruct the targeting molecule into little pieces and scatter them around the fluorophore so that in three dimensions it's a very compact molecule,» says Frangioni, who coined the novel arrangement «structure - inherent targeting.»

(b) Proteins in contact with a bifunctional lipid are tagged in vivo by photoaffinity labeling and then derivatized in vitro with biotin or a fluorophore using click chemistry, allowing their purification or visualization.

Use fluorophores with a narrow emission spectrum in multi-color IF experiments to avoid spectral overlap

In Figure 4, Panel B, cells were incubated simultaneously with anti ‑ SSEA ‑ 4 and anti - TRA ‑ 1 ‑ 60 antibodies, since they were labeled with different fluorophores (PE and FITC, respectively).

Structure - guided engineering of a Pacific Blue fluorophore ligase for specific protein imaging in living cells.

FITC / TRITC fluorophore tagged secondary antibodies (1:4000 dilutions) was added for 1 hour at RT in dark.

The increase in signal is not due to a brighter FP, but rather because of the correct folding and fluorophore formation by most, if not all, of the FP fusions.

Ligand - mediated receptor assembly was measured by photon transfer from the photon donor to the fluorophore resulting in fluorescence emission.

Apply an appropriate fluorophore - conjugated secondary antibody diluted in antibody dilution buffer to the coverslips and incubate for 1 hour in a moist, dark environment.

The result is illumination from the microlens disk through the pinhole disk for strong excitation of fluorophores, causing a fluorescence emission which in turn passes through the pinhole disk with high confocality.

PA - FPs, especially the monomeric, reversible type, are very well suited for super resolution imaging, where the power of the technique relies heavily on the same fluorophore being imaged multiple times in order to reconstruct an image.

First, the imaging of Brainbow mouse tissue was challenging due to low fluorescence intensity, caused partially by fluorophore photoinstability, as well as the tendency of fluorophores to aggregate in the neurons» somata.

Single cell suspensions were washed in PBS and then stained for 20 — 30 min on ice in the dark with fluorophore - labeled Abs (for a complete list see Tables 1 and 2) and fixable viability dye (eBioscience).

In addition, we will improve our possibilities to fluorescently label molecules in intact cells by using faster and more complete bioorthogonal reactions and new fluorophoreIn addition, we will improve our possibilities to fluorescently label molecules in intact cells by using faster and more complete bioorthogonal reactions and new fluorophorein intact cells by using faster and more complete bioorthogonal reactions and new fluorophores.

In addition to this, the use of multiple fluorophores can add a second layer of contrast, color contrast, to an image and thereby provide molecular or structural specificity to the viewer.

By coupling fluorophores to proteins, pioneers of this technique were able to directly detect protein / protein interactions in living cells.

(2015) Fluorophore - NanoLuc BRET reporters enable sensitive in vivo optical imaging and flow cytometry for monitoring tumorigenesis.

Unfortunately cross-talk between fluorophores does exist in the real world and corrective approaches and appropriate controls are required to make this method useful for dynamic experiments in which FRET changes are large.

Schaub, F.X., et al. (2015) Fluorophore - NanoLuc BRET reporters enable sensitive in vivo optical imaging and flow cytometry for monitoring tumorigenesis.

In their JCB 2003 paper, Sekar and Periasamy defined FRET as «a distance - dependent physical process by which energy is transferred non-radiatively from an excited molecular fluorophore (the donor) to another fluorophore (the acceptor) by means of intermolecular long - range dipole - dipole coupling.»

Unfortunately cross-talk between fluorophores does exist in the real world and corrective approaches and appropriate