His research interests include near - infrared
fluorophores as research tools and for fluorescence guided surgery.
Not exact matches
His group developed fluorescent molecules (
fluorophores)-- which he dubs «beacons» — and the team inserted them into the virus's outer covering, known
as the envelope.
«The conventional way to create a targeted
fluorophore is to take a targeting ligand, such
as an antibody, and hook it up to the lightbulb to create a new hybrid molecule.
Secondary antibodies are often conjugated to substances that will enable the visualization of the primary antibody, such
as HRP or
fluorophores.
For certain filters, e.g., FITC / TRITC filters, the compatibility evaluation is quite easy
as they have been named after the
fluorophores they are meant to be used with.
The H - 2Dbgp33 — 41 MHC - peptide tetramer was used for Ag - specific CD8 T cell identification and was provided
as fluorophore - conjugated tetramers by E.J.W.. All samples were acquired on a MACSQuant flow cytometer (Miltenyi Biotec) and analyzed using FlowJo software version 10.
First, the imaging of Brainbow mouse tissue was challenging due to low fluorescence intensity, caused partially by
fluorophore photoinstability,
as well
as the tendency of
fluorophores to aggregate in the neurons» somata.
TIRF is also very gentle on the sample
as the majority of the laser light is reflected away from the sample and excitation occurs through the
fluorophore's interaction with an evanescent energy field.
Homogeneous Time - Resolved Fluorescence (HTRF) Utilizing rare - earth lanthanides with long emission half - lives
as donor
fluorophores, HTRF technology combines standard FRET with the time - resolved measurement (TR) of fluorescence.
In their JCB 2003 paper, Sekar and Periasamy defined FRET
as «a distance - dependent physical process by which energy is transferred non-radiatively from an excited molecular
fluorophore (the donor) to another
fluorophore (the acceptor) by means of intermolecular long - range dipole - dipole coupling.»