Sentences with phrase «foetal rpe»
The explant model was incubated in Human foetal RPE medium at 37 °C in 5 % CO2 for 3 or 12 hours.
http://journals.plos.org/
Cells were cultured in human foetal RPE medium [30] where, within 13 days, they formed a pigmented monolayer with characteristic RPE cobblestone appearance similar to that observed in human post-mortem RPE sheets and HESC - derived RPE cells [9](Fig. 1B).
Labelled outer segments were washed, resuspended in human foetal RPE medium and seeded onto iPS - RPE cells cultured on gelatin - coated 35 mm dishes.
Total RNA was isolated from iPS, iPS - RPE and foetal RPE cell cultures using the Qiagen RNeasy Mini Kit (Qiagen, Valencia, CA).
Various cell types have been examined for use in RPE cell replacement including immortalized cell lines, such as the human RPE cell line, ARPE19 [2], sheets of adult RPE [3], foetal RPE [4], RPE derived from human embryonic stem cells (HESC - RPE)[5]--[10] and many non-RPE cells lines [11]--[15].