Sentences with phrase «followed by antibody»

Not exact matches

That was followed by the takeover of Kairos Therapeutics, a Vancouver startup re-engineering human antibodies to fight cancer.

Following, there is an irregular action of the immune system where the outcome is an antibody - antigen reaction, which leads to secreting lymphokines by T - lymphocytes.

Diego Grassi, PhD, a research associate in Lasmézas» lab, made this discovery by labeling the pα - syn * with an antibody so he could follow it throughout the cell after it was created.

Follow - up studies showed that the two antibodies isolated from the Ebola patient work by interfering with a critical step in the process by which ebolaviruses infect cells and then multiply inside them.

«I envision a treatment that uses a precise combination of sclerostin antibodies to grow new bone, followed by bisphosphonates to lock in that bone growth,» said Michelle Caird, associate professor of orthopaedic surgery who specializes in brittle bone disease.

They found substantial differences following influenza vaccination in the production of immune - modulators that determine the type of T - cell response and in the proliferation and production of antibodies by B cells.

Following transmission by mosquitoes, the early specific (also called adaptive) immune response to WNV is thought to be dominated by antibodies, and, consistent with this, the researchers found that older mice had less potent WNV - specific antibody responses during the early phase of infection.

Two injections of DNA followed by one injection of protein, or two injections of protein, produced large amounts of virus - eliminating antibodies in mice and monkeys.

It is composed of two elements: treating the patient with antibodies on the day of transplant, followed by a donor bone marrow infusion several days later.

For the other experiments, recombinant HA (His - tagged) was immobilized at 5 µl / min − 1 on NTA sensor chips with a target density of 350 response units, and the antibodies at concentrations ranging from 1 to 30 nM in HBS - P buffer were injected at 20 µl / min − 1 over the immobilized recombinant HA or reference cell surface, followed by a 600s dissociation phase.

Clinical samples were also sent to an outside laboratory (Focus Diagnostics, Cypress, CA) for respiratory viral testing by centrifugation - enhanced shell vial culture followed by direct fluorescent antibody staining for 8 viruses (respiratory syncytial virus, adenovirus, influenza virus A and B, parainfluenza virus types 1, 2, and 3, and human metapneumovirus).

You are correct, you need a secondary antibody that is conjugated to a fluorescent molecule (or an enzyme, followed by the enzyme's substrate to get...

The putative DN cells were isolated by negative selection using anti-CD8 magnetic bead isolation followed by in vitro treatment with anti-CD8 antibody and complement.

To meet this end, CCIR members take advantage of resources offered by MD Anderson, including the CCSG Shared Resources, Immune Monitoring Core Laboratory and GMP Cell Facility, allowing them to follow the «bench - to - beside» approach to develop and improve cancer immunotherapy in the form of cellular therapy, a vaccine or targeting antibody, either alone or in combination with conventional therapies or newly developed therapies.

arabidopsis, rice, wheat, corn whole plant tissue were subjected to SDS PAGE followed by western blot with 60004 -1-Ig (GAPDH Antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours

zebrafish tissue were subjected to SDS PAGE followed by western blot with 60004 -1-Ig (GAPDH antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours

Dr. Polonis» lab has focused for many years on functional antibodies elicited by natural infection and by vaccination, utilizing samples from multiple natural history cohorts and vaccine studies; her lab is currently performing an extensive analysis of pseudovirus neutralizing antibodies in the large RV144 follow - on trial, RV306.

mouse colon tissue were subjected to SDS PAGE followed by western blot with 11176 -1-AP (HNRNPA1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours

Following fixation in 4 % PFA, the cells were assessed for Sall4 expression using 1:100 dilution of the primary antibody (ab29112) in 1 % serum, 0.1 % triton, 0.1 % BSA in PBS, followed by detection using goat polyclonal rabbit IgG Alexa 488 (ab150077) at 1:200.

Rat brain tissue lysate was subjected to SDS PAGE followed by western blot with 23449 -1-AP (UBQLN2 antibody) at a dilution of 1:800 incubated at room temperature for 1.5 hours.

mouse testis tissue were subjected to SDS PAGE followed by western blot with 10837 -1-AP (OPTN antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours

The membrane was blocked with 3 % BSA / PBS for 1 h at RT followed by incubation with the corresponding primary antibody in 1 % BSA / PBS - T overnight at 4 °C: pTyr (Millipore, # 05 - 321, 1:500), Tie2 (R&D, #AF313, 1:1,000), Tie2 (R&D, #AF762), pAKT (Cell Signaling, # 4060S, 1:1,000), AKT (Cell Signaling, # 9272S, 1:1,000), FOXO1 (Cell Signaling, # 9454, 1:1,000), pFOXO3A (Cell Signaling, # 9466, 1:1,000), FOXO3A (Cell Signaling, # 12829, 1:1,000), VEGFR2 (Cell Signaling, # 2479, 1:1,000), tubulin (Sigma, #T8203, 1:5,000).

soybean whole plant tissue were subjected to SDS PAGE followed by western blot with 60004 -1-Ig (GAPDH Antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours

Raji cells were subjected to SDS PAGE followed by western blot with 60004 -1-Ig (GAPDH antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours

A549 cells were subjected to SDS PAGE followed by western blot with 60008 -1-Ig (ACTB antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours

HeLa cells were subjected to SDS PAGE followed by western blot with 11176 -1-AP (HNRNPA1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours

Historically, many vaccines have been designed to evoke an antibody response by B cells following innoculation with weakened or dead pathogens.

HEK - 293 cells were subjected to SDS PAGE followed by western blot with IFT88 antibody (13967 -1-AP) at a dilution of 1:1000 incubated at room temperature for 1.5 hours.

Confirmation of CD44 positive population post treatment was performed by incubating cell suspension with CD44 primary antibody (Abcam)(1:500 dilution) followed by FITC / TRITC tagged secondary antibody (Abcam)(1:1000 dilution).

MCF7 cells were subjected to SDS PAGE followed by western blot with 60008 -1-Ig (ACTB antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours

mouse brain tissue were subjected to SDS PAGE followed by western blot with 10837 -1-AP (OPTN antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours

arabidopsis whole plant tissue were subjected to SDS PAGE followed by western blot with 60008 -1-Ig (beta actin Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours

In the Collagen Antibody Induced Arthritis (CAIA) model, arthritis is stimulated through the administration of a cocktail of monoclonal antibodies that are directed to conserved auto - antigenic epitopes of collagen type II, followed by endotoxin challenge.

Immunization with the 3 dose levels was followed by a dramatic rise in anti-AMA1 antibodies, significantly higher than in the control group after a single immunization.

HuH - 7 cell extracts (lane 3) or FLAG - Sp1 - containing cell extracts (lane 4; 1.5 mg each) were immunoprecipitated with anti-FLAG antibody — conjugated beads and the immunoprecipitates were analyzed by SDS - PAGE followed by immunoblotting with anti-FLAG antibody or anti-DDX3 antibody.

Levels of anti-AMA1 antibodies measured by ELISA increased significantly (at least 100-fold compared to baseline) in all 3 malaria vaccine groups, and remained high during the year of follow up.

rat brain tissue were subjected to SDS PAGE followed by western blot with 21672 -1-AP (SIP1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours

This was followed by overnight incubation with primary antibodies for IL - 6 (Santa Cruz Biotechnology Inc., Santa Cruz, CA, USA), IL - 1β (Santa Cruz Biotechnology Inc.), TNF (BD PharMingen, San Jose, CA, USA), collagen (Santa Cruz Biotechnology Inc.), and amylase (Santa Cruz Biotechnology Inc.) at a dilution of 1:100 in a humified chamber.

After incubation with 3 % hydrogen peroxide for 10 minutes and 0.2 % horse serum for 20 minutes, a 1:200 diluted rabbit anti-DDX3 polyclonal antibody was applied for 1 hour at room temperature and followed by incubation with biotinylated linker and streptavidin - horseradish peroxidase (LSAB2 system - HRP, DAKO Cytomation, Carpinteria, CA) for 30 and 10 minutes, respectively.

HepG2 cells were subjected to SDS PAGE followed by western blot with 21672 -1-AP (SIP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours

MCF7 cells were subjected to SDS PAGE followed by western blot with 10379 -1-AP (SNRPD3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours

Briefly, 1 mL cell suspension (1 × 106 cells) was incubated for few minutes in rat specific Fc block solution (BD Biosciences, San Jose, CA, USA) followed by incubation with fluorescence - tagged primary antibodies - CD11b - FITC (BD Biosciences), TNF - α - PE (BD Biosciences) and NF - κB - FITC (in 1:100 dilution) at 4 °C for 30 min in dark.

HeLa cells were subjected to SDS PAGE followed by western blot with 21672 -1-AP (SIP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours

(B) MNK - 3 cells were pretreated with anti-IL-23 p19 antibody (5 or 50 μg / ml) for 1 hr followed by IL - 23 stimulation for 15 or 30 min.

Immunofluorescent labeling was performed using anti-Olig2 (1 ∶ 4000, Chemicon) and anti-GFAP (1 ∶ 400, Cell Signaling) followed by fluorescently labeled, secondary anti-Ig antibodies (Alexa 488 and 568 conjugates, Invitrogen) at a 1 ∶ 2000 dilution.

The antibody - antigen interaction is visualized by adding a HRP conjugated secondary antibody followed by a chromogen (e.g. DAB (ab94665)-RRB- that yields a permanent brown precipitate.

Coverslips plated with acutely dissociated hNPCctx - GDNF or hNPCctx were fixed with 4 % paraformaldehyde, washed with PBS, blocked in 5 % normal donkey serum and 0.1 % Triton X-100, and incubated with goat anti-GDNF (1 ∶ 100; R&D Systems) primary antibody followed by donkey anti-goat Cy3 - conjugated secondary antibody (1 ∶ 1000; Jackson IR).

TPH2 (55 KDa) was detected using a goat anti − mouse IgG horseradish peroxidase - conjugated secondary antibody (1:10 000; Bio-RAD, Hercules, California) followed by Chemiluminescence ECL (PerkinElmer, Waltham, Massachusetts) and quantified from densitometry bands (MCID Elite 6; Imaging Research Inc, Ontario, California) standardized to human adult raphé obscurus run on the same gel.

One year follow - up revealed that AN1792 antibody responders showed improvements in cognitive measures as assessed by the neuropsychological test battery (NTB) and a decrease in brain volume compared with placebo.

In addition to staining the midgut contents with FITC - labeled polyclonal anti-Borrelia species antibody, we washed and re-stained this set and stained the second set of xenodiagnostic tick (XT) samples with an anti-OspA monoclonal antibody (CB10, obtained from J. Benach [41]-RRB-, followed by anti-mouse IgG - Alexa 488 (Molecular Probes).