Sentences with phrase «followed by centrifugation»
The mixture was sonicated on ice with 0.1 % Triton X-100 for 30 min, followed by centrifugation at 13,000 rpm for 30 min.
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The extracts are filtered through PVDF syringe filters (0.2 micron pore) followed by dilution with acetonitrile and a second centrifugation at 16100 g.
Peripheral blood mononuclear cells were isolated by centrifugation with 96 % Ficoll (BD), followed by erythrocyte lysis with ACK lysis buffer.
Clinical samples were also sent to an outside laboratory (Focus Diagnostics, Cypress, CA) for respiratory viral testing by centrifugation - enhanced shell vial culture followed by direct fluorescent antibody staining for 8 viruses (respiratory syncytial virus, adenovirus, influenza virus A and B, parainfluenza virus types 1, 2, and 3, and human metapneumovirus).
Bone marrow - derived mesenchymal stem cells (BM - MSCs) are obtained by isolating mononucleated cells from a marrow aspirate by centrifugation on a density gradient, followed by adherent cell expansion in tissue culture.
Bacteria were allowed to express the recombinant protein overnight at room temperature, followed by harvesting by centrifugation at 6000 rpm for 10 min.