Sentences with phrase «following polyclonal»

A Th2 profile with increase in the numbers of CD4 + and CD8 + T cells secreting IL - 4 following polyclonal stimulation, including in resting cells, was demonstrated in CFS patients [13].

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Membranes were probed with the following primary antibodies diluted in TBS - T with 5 % NFM overnight at 4 °C: calsequestrin rabbit polyclonal antibody (1:2,000 dilution, ab3516, Abcam), PPARδ rabbit polyclonal antibody (2 μg • ml — 1 dilution, ab23673, Abcam), COX polypeptide Va mouse monoclonal antibody (1:1,000 dilution, MS409, MitoSciences), phosphorylated Smad2 (1:1,000 dilution, 3101, Cell Signaling Technology), Smad2 / 3 (1:1,000 dilution, 3102, Cell Signaling Technology), GAPDH rabbit polyclonal antibody (1:1,000 dilution, ab9485, Abcam).

Hematopoietic malignancies were diagnosed using the following markers: for B - and T - lymphocyte detection, rat antimouse CD45R / B220 mAb (Southern Biotechnology Associates, Birmingham, AL; dilution 1:100); for T - lymphocyte detection, rabbit antihuman CD3 polyclonal antibody (Dako, Glostrup, Denmark; 1:100); and for monocyte / macrophage detection, rat antimouse F4 / 80 mAb (BMA Biomedicals AG, Augst, Switzerland; 1:20) and rat antimouse CD11b mAb (Chemicon International, Temecula, CA; 1:20).

Following fixation in 4 % PFA, the cells were assessed for Sall4 expression using 1:100 dilution of the primary antibody (ab29112) in 1 % serum, 0.1 % triton, 0.1 % BSA in PBS, followed by detection using goat polyclonal rabbit IgG Alexa 488 (ab150077) at 1:200.

The development of anti-galectin 3 polyclonal responses in pancreatic cancer patients after vaccination with GVAX (2) or prostate cancer patients following Sipuleucel - T (3) has been associated to prolonged survival, thus providing an supportive rationale for targeting galectin 3.

After incubation with 3 % hydrogen peroxide for 10 minutes and 0.2 % horse serum for 20 minutes, a 1:200 diluted rabbit anti-DDX3 polyclonal antibody was applied for 1 hour at room temperature and followed by incubation with biotinylated linker and streptavidin - horseradish peroxidase (LSAB2 system - HRP, DAKO Cytomation, Carpinteria, CA) for 30 and 10 minutes, respectively.

In addition to staining the midgut contents with FITC - labeled polyclonal anti-Borrelia species antibody, we washed and re-stained this set and stained the second set of xenodiagnostic tick (XT) samples with an anti-OspA monoclonal antibody (CB10, obtained from J. Benach [41]-RRB-, followed by anti-mouse IgG - Alexa 488 (Molecular Probes).

The following goat polyclonal antibodies from Santa Cruz Biotechnology Inc. were used for ChIP: anti-DAXX (sc - 7001) and anti-DNMT1 (sc - 10219).