The TUNEL assay
for apoptotic cell detection was performed using the In Situ Cell Death Detection Kit (Boehringer Mannheim GmbH) and was performed according to the manufacturer's instructions.
Annexin V - FITC staining was used as a marker
for apoptotic cells, while PI staining was used as an indicator of necrotic cells that had lost membrane integrity.
Not exact matches
Also covered will be techniques
for analyzing exosomes, microvesicles, and
apoptotic bodies in unprocessed samples, how imaging flow cytometry can be used to evaluate or reevaluate EV isolation techniques, and the advantages and disadvantages of using this method.
Importantly, while the enhanced presence of Bcl - xL is expected to promote cancer cell survival in the first place, it may also indicate that the
apoptotic machinery (especially the caspases) are still in place, making it necessary
for the tumor cell to maintain a high level of Bcl - xL [10].
A protein domain that is found in certain initiator caspases (
for example, mammalian caspase - 9) and their adaptor proteins (
for example,
apoptotic - protease - activating factor 1, APAF1).
For example, by engineering PA - GFP tagged to a mitochondrial matrix protein (mito - PAGFP) the Youle Lab was able to visualize and quantify the fusion dynamics of mitochondria in healthy and
apoptotic cells (9).
Sciorati C, Monno A, Ascherman DP, Seletti E, Manfredi AA, Rovere - Querini P. Required role of
apoptotic myogenic precursors and toll - like receptor stimulation
for the establishment of autoimmune myositis in experimental murine models.
Symposia X: The Impact of Heat Shock Protein Expression on Muscle Metabolism, Exercise Capacity and Disease Prevention Speakers will present evidence
for the functional effects and mechanisms of heat shock proteins and their role in aging, mitochondrial function,
apoptotic pathways, insulin sensitivity, myocardial protection and more.
Mutations in RNAse L that impair the
apoptotic response to viral infection were postulated to reflect enhanced susceptibility to infection by XMRV and suggested a potential role
for the virus in carcinogenesis [5], [6], [7], [8].
* please note this kit can not be used
for the amplification of
apoptotic DNA (160 - 170 bp), please see our TruePrime ™
apoptotic cell - free DNA amplification kit.