The TUNEL assay
for apoptotic cell detection was performed using the In Situ Cell Death Detection Kit (Boehringer Mannheim GmbH) and was performed according to the manufacturer's instructions.
Annexin V - FITC staining was used as a marker
for apoptotic cells, while PI staining was used as an indicator of necrotic cells that had lost membrane integrity.
Not exact matches
Importantly, while the enhanced presence of Bcl - xL is expected to promote cancer
cell survival in the first place, it may also indicate that the
apoptotic machinery (especially the caspases) are still in place, making it necessary
for the tumor
cell to maintain a high level of Bcl - xL [10].
For example, by engineering PA - GFP tagged to a mitochondrial matrix protein (mito - PAGFP) the Youle Lab was able to visualize and quantify the fusion dynamics of mitochondria in healthy and
apoptotic cells (9).
* please note this kit can not be used
for the amplification of
apoptotic DNA (160 - 170 bp), please see our TruePrime ™
apoptotic cell - free DNA amplification kit.