Sentences with phrase «for cell lineage»

Not exact matches

To the contrary, it is more fantastic than we can imagine — hundreds of billions (trillions) of galaxies with hundreds of billions (trillions) of stars, nearly all of which have planets, some right for life; planets so hot that they rain glass; stars made of diamonds; the lineage of animals from singled celled organisms to the incredible variety that exists today with their unique adaptations.

All of these steps can be carried out as quickly as images are acquired by the microscope, and the result is lineage information for every cell.

Cohen, whose background includes developing operating systems support for computer games, has developed a program called LEVER - short for Lineage Editing and Validation program - that can identify, tag and track live cells, capturing patterns of motion and cell division, using sequences of microscopic time - lapse images.

Blood founder cells showed a much lower propensity for aberrant methylation than did other founder cells, which could explain why in the past scientists attributed the founder cell to the effectiveness of differentiating iPS cells to the hematopoietic lineage.

Foremost is that it is one of the first to show the benefits of using human iPS cell - derived renal lineage cells for cell therapy.

Mice from one vendor, for example, were notably lacking segmented filamentous bacteria (SFB), a commensal lineage recently shown to help mice produce key antibodies and immune cells in their intestines.

The team's model, still in its early stages, is hoped to form a foundation for continuing studies on germ cell lineage.

It provides insights into how to transform stem cells into therapeutically useful cells and suggests possible new treatments for leukemias, in which a persistent mixed lineage seems to drive cancerous proliferation.

The question is now whether these cells, these pericytes are also permissive for direct lineage reprogramming?

COURTESY SHENDURE AND SCHIER LABS CRISPR FOR FATE - MAPPING Researcher: Jay Shendure, Professor, Department of Genome Sciences, University of Washington Project: In collaboration with Alexander Schier's lab at Harvard University, Shendure's group came up with a new way to trace cell lineages in cell culture and in whole organisms — in this case, developing zebrafish.

The second step will be to optimize culture conditions for the commitment of the iPS / hES cell - derived retinal precursors towards the photoreceptor lineage.

For example, intra-strain cooperative behaviors are more likely to be evolutionarily stable when different cell lineages are segregated in space, with typical interaction distances between cells being strongly influenced by the diffusivity of secreted products, biofilm architecture, and environmental flow conditions [16 — 19].

For example, cross-model comparisons may help to pinpoint key cell types and molecules involved in lineage decisions, reveal evolutionary inventions, and may allow to interpret genetic disease models (for example in mouse) by mapping to human or other systeFor example, cross-model comparisons may help to pinpoint key cell types and molecules involved in lineage decisions, reveal evolutionary inventions, and may allow to interpret genetic disease models (for example in mouse) by mapping to human or other systefor example in mouse) by mapping to human or other systems.

This makes a strong case for the utility of lineage through program cells for diseased modeling.

The emergent spatial structure of cell lineages during biofilm growth is important to numerous other facets of microbial ecology, especially for the evolutionary trajectories of social phenotypes [16].

We seek to «Track and Treat» the cancer cells present in patients» tissues by using lineage tracing, as well as sequencing technologies, to determine the molecular mechanisms responsible for the dissemination of cancer cells to various organs, and resistance to current therapies.

Diagnosis of BPDCN was confirmed by the positivity of the cells for CD4 and CD56, along with other markers that are more restricted to plasmacytoid dendritic cells (such as CD123), and the negativity of the cells for lymphoid, NK and myeloid lineage - associated antigens.

Currently, we are exploring several environmental samples retrieved from allover the world - ranging from hydrothermal vents in Japan to hot springs in Yellowstone National Park and New Zealand — for the presence of novel archaeal (and bacterial) lineages using cultivation - independent approaches, such as metagenomics and single cell genomics.

Human mesenchymal stem cells (hMSCs) are currently the most common adult stem cell type used for cell therapy applications due to their regenerative properties and ability to differentiate into multiple cell lineages (adipocyte, chondro ¬ cyte, and osteocyte).

The cell produces nerve cells that help form the neocortex — the site of higher cognitive function — and likely accounts for the dramatic expansion of the region in the lineages that lead to man, the researchers say.

When the ESCs had no Chd1 at all, the cells could not differentiate into all of the three germ layers (specifically, endoderm was not detected, and a preference was found for neuronal lineages).

Dr Véronique Azuara has worked for several years in the field of developmental biology and genetics moving from lymphocyte development (PhD training at the Pasteur Institute — Paris) to epigenetic studies (post-doctoral training at the MRC / Clinical Sciences Centre — London) to explore how chromatin shut down contributes to lineage restriction and maintenance of cell fate identity through development.

The resultant clones with functional FIX can then be screened for integration of the viral backbone in safe harbor regions and for its robust expression, subsequent to which they will be differentiated in vitro to the hepatic lineage, with a view to producing a cell - based therapy for hemophilia.

The most compelling argument for an inflammatory role has come from studies of transgenic mice that over express LIGHT in the T cell lineage.

SSEA - 1 was used as common marker for differentiated cells since the aim was not to address towards which lineages the cells were differentiating.

This in vivo lineage reprogramming breakthrough may lead to a vast new and safer source of replacement cells for degenerative diseases and injuries.

The B cell depleted fraction was then surface stained for lineage markers (CD3, CD19, CD14), CD127 and cells were sorted (Lin - CD127 +).

Further, we have demonstrated for the first time that a cell's identity can be reprogrammed to convert into a completely unrelated lineage, without their removal from the body (in vivo) and without passage through a stem cell intermediate.

Through extensive examination, we have shown that human NSC - derived iPS cells are phenotypically identical to hESCs by all pluripotential markers analyzed and for their ability to differentiate into all three major germ lineages.

Thus while SSEA - 1 may be an effective marker for undifferentiated cells when used in combination with a PrEn marker, its utility may be limited to this lineage.

We established several cell lineages from three independent transfection experiments and chose three lines (iPSC1, iPSC2, iPSC3) for further characterization.

In addition, well - characterized expression profiles for melanoma cells have been identified that correlate highly proliferative cell states with increased expression for pathways regulated by the lineage - specific transcription factors SOX10 and MITF; conversely, migratory / invasive cell states have been correlated with TGFβ1 signaling pathways.

To induce mesoderm and endoderm lineages from iPS cells, cells were grown as embryoid bodies (EB) in DMEM / F12 with 10 % fetal bovine serum for 2 weeks.

That this occurs once cells enter differentiation, is consistent with a requirement for sustained FGF signalling for commitment and segregation of the PrEn lineage in cultured blastocysts [57].

In this way embryo - derived stem cell lines and ES cell differentiation may be providing access to potential «transition states,» required for lineage specification in vivo.

Demonstrated that not a single «master» transcription factor, but rather a combination of factors, are important for reprogramming of cell fate from one somatic lineage back to a pluripotent state.

Dr. Shendure's research group in Seattle pioneered exome sequencing and its earliest applications to gene discovery for Mendelian disorders (e.g. Miller and Kabuki syndrome) and autism; cell - free DNA diagnostics for cancer and reproductive medicine; massively parallel reporter assays and saturation genome editing; whole organism lineage tracing; and massively parallel molecular profiling of single cells.

Indeed this sort of model has been used to explain the existence of a stable attractor and associated lineage primed states in EML cells, a haematopoietic progenitor cell line [54], and as the basis for heterogeneity in Nanog expression in ES cells [11].

One possibility is that V+S + cells produce additional factors required for these lineages.

Zebrabow: multispectral cell labeling for cell tracing and lineage analysis in zebrafish.

Cell lineage tracing analysis helped researchers identify early endothelial cells as the cell of origin for the tumor subtCell lineage tracing analysis helped researchers identify early endothelial cells as the cell of origin for the tumor subtcell of origin for the tumor subtype.

The existence of multiple sub-states within a single ES cell basin of attraction or multiple interrelated attractors representing distinct lineages could account for pluripotency.

We believe that a similar early precursor may exist to the PrEc lineage (Figure 9), and while we have no direct evidence for this, we did observe Oct4 positive cells that neither expressed Nanog nor the Venus transgene and there also appears a slight enrichment of early neural markers in the V − S + population (Figure 4).

Our group will be responsible for an entirely automated cell culture platform, the CompacT SelecT devoted to the amplification of hES and hiPS cells and their differentiation into various cell lineages.

Based on previous protocols [5, 6] they have now created a 3D protocol for chondrogenic lineage differentiation via the generation of a putative chondrogenic progenitor cell population, and have found that using this protocol C - iPSCs can be readily differentiated into cartilage in a manner comparable to that of mature chondrocytes [7].

Our results also suggest that such a strategy is rich in the potential of identifying lineage specific reagents and provides additional evidence for the utility of stem cells in screening and discovery paradigms.

For example, assaying the genomes of multiple cells during development reveals developmental lineage trees - the kinship lineage - whereas cellular transcriptomes inform us about the regulatory state of cells and their gradual restriction in potency - the Waddington lineage.

Importantly, this approach is adaptable for use in a Chemistry, Manufacture and Control drug screening protocol and may have applications in identifying lineage specific reagents, thus providing additional evidence for the utility of stem cells in screening and discovery paradigms.

Bivalent marking of loci encoding lineage specific transcription factors in ES cells is thought to prepare these genes for rapid activation of transcription once the pluripotency network is extinguished [4].

These workers considered that this fluctuation might account for reversible lineage priming towards specific cell fates.