We acknowledge the OMRF FACS core and the OUHSC Flow Cytometry and Imaging Core
for cell sorting and analysis.
The facility's staff will provide information for the preparation of samples
for cell sorting.
The emission wavelength used
for cell sorting of DiI - Ac - LDL - labeled cells was 550 nm.
Using a high - speed actuator as the driving source of pump, they succeeded in producing a flow with 16 microseconds
for cell sorting.
Not exact matches
• NanoCellect Biomedical, a San Diego - based developer of microfluidic
cell sorting technologies
for cell - based assays, raised $ 10 million in series B funding.
Internet connectivity is delivered to planes either by expensive ground - based
cell towers or via even more expensive satellite relays, which means there will always be a significantly higher cost
for this
sort of access than there is
for the terrestrial equivalent.
This depends upon there being a brain, an arrangement of
cells in a particular part of the body which by reason of its peculiar coordination makes the given routing able to «know» in a distinctively human manner — quite different from, although certainly continuous with, the
sort of «knowing» that is possible
for the higher grades of animal life.
Stem
cells can transform into any other human
cells, so they have immense potential
for generating all
sorts of adult
cells and thus can be used in research concerning human degenerative (and other) diseases.
I've known that in a general
sort of way
for a long time, but the concept really hit me earlier in the semester when I started taking biology and learning more about
cells and nutrition.
In that case, your child won't be «ready»
for a
cell phone until she is able to earn some
sort of income.
BEHAVIOUR The Downside of Time - Out RECIPES Strawberries
for Dessert Cake & Strawberry Kabobs PETS Chillin» with your Dog INFANTS Sleep Baby Sleep LANGUAGE Raising a Bilingual Child PARENTING Rethinking Our
Cell Phone Use FATHERING The Ultimate Camping Greenhorn WELL BEING Mud — the New Kids» Medicine 15 MINUTE MOM
Sorting Through School Year Clutter More Blogs CONTESTS Go Treetop Trekking Centreville Passes Boston Pizza More Contests
This part of the
cell then becomes an intensive production shop of
sorts, generating the proteins needed
for processing the food.
Called SIF - seq,
for site - specific integration fluorescence - activated
cell sorting followed by sequencing, this new technique complements existing genomic tools, such as ChIP - seq (chromatin immunoprecipitation followed by sequencing), and offers some additional benefits.
Eager to
sort through the pile of curriculum vitae's on his desk, Rudnicki is particularly looking
for molecular geneticists working on different stem
cell systems; scientists with genomics bents like proteomics, microarray analysis, and bioinformatics; and a couple of clinician scientists.
Using a powerful microscope to observe mouse oocytes as they split, Ellenberg's group found that the spindles assembled into two coherent structures, one
for the future egg and one
for the future polar body.At first, spindles appeared throughout the
cell in a
sort of mesh.
Those qualities make graphene a tantalizing alternative
for use as a transparent conductor, the
sort now found in everything from computer displays and flat panel TVs to ATM touch screens and solar
cells.
Next, they used a technique called fluorescence - activated
cell sorting to separate out the newly differentiated retinal ganglion
cells from a mixture of different
cells into a highly purified
cell population
for study.
The
sorting of individual
cells is necessary
for many biological applications, including the isolation of specific
cell types from
cell suspensions.
The University of Hawai'i Office of Technology Transfer and Economic Development (OTTED) has filed a provisional U.S. patent application
for the new
cell -
sorting method, «Surface Free Energy Based Particle Sorting.
sorting method, «Surface Free Energy Based Particle
Sorting.
Sorting.»
A couple of other research groups have also
sorted stem
cells based on gene expression, but they have not done so specifically with the goal of enriching
cell populations
for a specific tissue, the researchers said.
Nevertheless, he thinks this
sort of technology will be seen in the clinic before a treatment based on a stem
cell replacement
for photoreceptors.
The bone matrix being produced by
cells is stained red in samples of
cells that are
sorted for not expressing ALPL (left),
sorted for expressing ALPL (right), and left as an unsorted control (center).
This chip has microchannels into which
cell suspensions are introduced
for sorting.
George Daley, speaking of potential therapeutic testing of parthenote - derived stem
cells (the
sort that his lab reported isolating last December), said it was «reasonable» to assume that the technology wouldn't be ready
for human testing
for another seven years.
A fluorescence - activated
cell sorting (FACS) has been used
for high - throughput
cell sorting.
An antibody that works well
for Western blots on denaturing gels,
for example, should not be presumed to work equally well in nondenaturing assays such as fluorescence - activated
cell sorting.
Quiroga's hippocampal
cells, just a few neural connections away, are like the output of that table — a
sort of speed dial
for people and concepts previously encountered.
«It's the same process as
for single
cell genomics, but for aggregates of symbiotic bacteria and archaea,» said DOE JGI Microscale Applications Group head Rex Malmstrom of the technique called BONCAT - FACS (BONCAT — Fluorescence - Activated Cell Sorti
cell genomics, but
for aggregates of symbiotic bacteria and archaea,» said DOE JGI Microscale Applications Group head Rex Malmstrom of the technique called BONCAT - FACS (BONCAT — Fluorescence - Activated
Cell Sorti
Cell Sorting).
They have screened combinations of antibodies that bind to specific proteins on the surface of the immature and mature stem
cells and that can be used
for flow cytometry, a common laboratory technique
for sorting cells.
Such measurements could be used to
sort different types of
cells — to distinguish cancerous
cells from healthy ones,
for example — or to measure how
cells change when exposed to drugs.
Traditional methods
for sorting cells rely on fluorescent molecular tags, designed to stick to the surface proteins present on certain types of
cells but not on others.
«We now have a simple tool
for identifying and
sorting the
cells, which benefits future stem
cell research and basic research on early embryonic development.»
We have shown that this technology can be applied to solve the atomic scale details
for all
sorts of fundamental pathways going on in
cells, opening the way to direct applications
for medical research» he said.
Aeolian deposition is responsible
for sorting and transporting lithogenic matter (primarily sand - and clay - sized particles), containing microbial
cells from the surrounding desert environments (soils, ephemeral streams, glaciers, etc.) onto the ice covers that range in thickness from 3 to 20 meters.
The mitochondrion is an organelle that produces the energy needed
for the
cell survival, operating as a
sort of power station.
«Even if a
cell takes a wrong turn, it is ultimately
sorted out again if its characteristics are unsuitable
for the niche, or slot, it has landed in,» says Skupin.
Because of the work of several other collaborators, Haughey says, his team knew that some
sort of inflammation - promoting molecule was released from brain and targeted to the liver after brain injury to send immune system
cells to the damaged area, but the identity of this go - between had been elusive
for years.
So,
for example, at the University of California in San Francisco they are trying to engineer E. coli so that it can detect cancer
cells, it can invade tumors, and then once it's inside they can release toxins; and so they are putting in all
sorts of genes from other bacteria to assemble this, you know, this
sort of synthetic E. coli that could become basically a cancer torpedo.
Lysosomes act as a
sort of recycling and disposal center
for cells.
The «small, but perfectly formed» flies will help scientists
sort out the mechanisms governing
cell size and number during development, says molecular biologist Sally Leevers of the Ludwig Institute
for Cancer Research in London.
Three men will share the honor
for their work describing how small membrane bubbles loaded with molecules get
sorted to the correct part of the
cell.
They feed on the bottom of the food chain — on single -
celled plankton, which larger fish can not eat — and then they become prey
for all
sorts of upper - level predators like tuna, sea bass and halibut as well as seabirds and marine mammals.
«Future
sorting techniques based on
cell stiffness and existing biomarkers may enable the enrichment of limbal stem
cells, thus facilitating clinical success
for patients with naturally low limbal stem
cell percentages.»
The group was then able to
sort the
cells using markers expressed on the
cell surface, to help pull out successfully edited
cells for research, and eventually
for therapeutic use.
«In combination with recent advances in
sorting cells on a biophysical basis, the biomechanical stemness markers we identified hold the potential to rapidly generate corneal transplants with highly enriched stem
cell populations, which could one day translate into better outcomes
for these patients.»
Unlike atomic force microscopy, microfluidics is a high - throughput screening technique, but additional work is required to assess the efficiency of this type of biophysical - based
sorting for stem
cell enrichment.
All of the following antibodies
for both analytical and
cell sorting cytometry were purchased from BD, except anti-CD27, which was purchased from eBioscience: anti — CD3 - PECy7 or PerCP, anti — CD20 - PECy7 or PerCP, anti — CD38 - PE, anti — CD27 - APC, and anti — CD19 - FITC.
The Mayo Clinic has received $ 3 million from the Glenn Foundation
for Medical Research to study how the presence of a specific
sort of human
cell affects aging.
Our success in these areas is complemented by our substantial investment in cutting - edge facilities
for nuclear magnetic resonance, protein crystallization, X-ray crystallography, transgenics, histochemistry, electron microscopy, confocal microscopy, and fluorescence activated
cell sorting.
Rapsyn is made by our muscle
cells and considered a
sort of biological anchor that interacts with the acetylcholine receptors to ensure that they are optimally positioned
for our muscles to receive orders from our brain.