Sentences with phrase «for deep sequencing»

We then expanded the project to the other two sites, from which we screened many more samples than we could sequence deeply, so we selected the best four samples (with highest complexity and endogenous DNA) from Oakington and the best from Linton (from which we had fewer samples, and there was only one sample with acceptable complexity for deep sequencing).

For the XMRV - positive and XMRV - negative prostate cancer samples, randomly amplified cDNA was prepared for deep sequencing using a variation of Illumina's TruSeq protocol (Illumina) as previously described [2], [45].

To facilitate whole - genome sequencing of TMAdV, we prepared amplified cDNA / DNA libraries for deep sequencing from lung tissue and a lung swab sample from 2 different monkeys using previously published protocols [23], [52].

Not exact matches

Frey likens Deep Genomics» technology to a Google search for genetic mutations: researchers can query a DNA sequence, and the system will identify mutations and tell them what's going to happen and and why it might cause a certain disease.

For example, if founders have established a company to tackle problems related to DNA sequencing yet prior experience is in social media, a deeper look into the team's motivations and understanding of its target market would be warranted.

On the Thursday before their mid-September game against Minnesota, the Colts prepared for that very situation with this three - play sequence, as narrated by Arians: «Deep in, timeout.

In the poignant final sequence, Gaskin exhibits her Safe Motherhood Quilt and calls for a deeper examination into the rising maternal mortality rate in the US.

In fact, Free Birth is about women taking full responsibility for our experiences, and re-wilding herself into a deep relationship of trust and autonomy, allowing the physiological and biological sequence of natural mammalian labor to unfold, undisturbed.

Researchers sequenced nuclear DNA from three molars from layer 11 and a child's molar from a deeper layer, 22, according to a talk by graduate student Viviane Slon, who works in the lab of paleogeneticist Svante Pääbo at the Max Planck Institute for Evolutionary Anthropology.

That's why he and hundreds of other scientists around the world have joined Earthtime, a 10 - year endeavor to nail down the sequence of past events on Earth by refining scientists» techniques for measuring deep time.

«This is a story that couldn't have happened five years ago, but with the evolution of deep sequencing, we were able to use the technology at just the right time and harness it with what we've been working on for decades,» he says.

But the benefits of this technology for discerning the circuits of the mind go much deeper, because the virus that carries the photoreceptor genes can also carry promoter sequences that express their payload only in neurons with the appropriate molecular address.

That began to change last year with the discovery of DNA sequences for an organism that no one has ever actually seen living near a deep - sea vent on the ocean floor.

Deep sequencing of isolates taken from a single patient carrying MRSA for the longest period revealed that although all isolates were of the same clade, there were small genetic differences between them, suggesting bacterial diversity within a single carrier.

«Tim and I were able to work very closely in all the steps of the project from the experimental design in the wet lab to the final analysis of the results; the major challenge was how to handle the very large number of barley samples and for this we designed a novel approach to sequencing that exploited deep results in combinatorics,» Lonardi said.

A study of this scope is possible thanks to innovative techniques for in vitro selection (such as mRNA display), as well as a revolutionary technology permitting massively parallel RNA sequencing (known as deep sequencing), which provides unprecedented speed and read accuracy.

«Our combined analysis of cell - free DNA and white blood cell DNA allows for identification of tumor DNA with much higher sensitivity, and deep sequencing also helps us find those rare tumor DNA fragments.»

«The only way to know for sure is to deep sequence them.»

This type of deep sequencing lets researchers search for minor variants in a virus that can acquire mutations and ultimately take over.

Extracted nucleic acid was amplified using a random PCR method to generate cDNA libraries for Virochip and deep sequencing analyses as previously described [18], [21].

Cell growth was monitored every 48 hours post-stimulation for approximately two weeks and the efficiency of CXCR4 disruption was assessed at day five post-transduction by both the Surveyor nuclease assay and by deep - sequencing of the CXCR4 target site.

This has implications for the use of deep sequencing analysis of immunization studies: if the sequence of J3 had been used as a reference to filter the sequences from llama 9 (without prior screening of VHH from llama 9), no J3 - like antibodies would have been found, 3E3 would not have been identified and it would have falsely appeared that the immunization of llama 9 had failed.

$ 2,500 «Beyond bacteria» Deeper shotgun metagenome and virome characterization of one sample, plus additional marker gene sequencing (16S rRNA, 18S rRNA and ITS, for you sequencing wonks) to characterize not just the bacteria but also the viruses, microbial eukaryotes (like giardia), and fungi in your gut.

For one thing, sequencing technologies continue to develop at a breakneck pace, and, according to Willerslev, as third - generation technologies such as nanopore sequencing are applied to ancient - DNA work, researchers will be able to probe even deeper into the biological past.

The sequence will provide a deeper understanding of the genome architecture and become a resource for biomarker discovery and development of diagnostic tools.

These individuals had already been deep sequenced with Illumina technology, both for variant calling and estimation of DNA methylation based on treatment with bisulphite.

The new deep sequencing methods allow data to be acquired much more efficiently and inexpensively, but optimal methods are less well developed (for some recent work in this area see [8, 14, 26]-RRB-.

New deep sequencing methods provide a convenient platform for characterizing the composition of the human microbiota [4, 7, 8, 13, 15 — 19].

As is often the case for yeast, the ability to sequence and analyze whole genomes at very deep coverage has yielded broad insights on eukaryotic genome evolution.

Over 100,000 genomes of individual humans (based on various estimates) have been sequenced allowing for deep insights into what makes individuals and families unique and what causes disease in each of us.

We often have to go through different lab processes and consider different analytical problems, such as the need for methods of deep sequencing from small clinical samples,» says Kwiatkowski.

On May 25, the team, which includes co-principal investigators Louis Whitcomb, a professor of mechanical engineering at The Johns Hopkins University, and Dana Yoerger of WHOI who were responsible for development of the vehicle's navigation and control system, conducted a planned sequence of successively deeper dives — testing Nereus, making scientific observations and collecting deep - sea samples at each depth they reached.

We provide consultation on study design and sample size calculation for array or deep - sequencing - based genomics studies.

Taken together, these results support the utility of VarScan for variant detection in next - gen sequencing, and demonstrate the feasibility of large - scale mutational profiling by deep resequencing of pooled samples.

A consensus sequence based on mapped deep sequencing reads was generated for each of the prostate cancer XMRV genomes and used to correct errors in the previously published sequences, with the requirement of no ambiguity at each discrepant nucleotide position.

Both of these cell line - associated XMRV genomes were found to exhibit a lower degree of intra-strain variation than previously reported for XMRV from 22Rv1 cells [20], with only 19 SNPs detected in the 22Rv1 - associated XMRV genome at the 3 % frequency cutoff by deep sequencing, and only 25 SNPs in the LNCaP - associated genome (Fig. 7A; Table S1).

Importantly, while the RNA from sample VP62 extracted in 2006 was positive for XMRV, RNA extracted from the same prostate tissue in 2012 was found to be negative not only by ViroChip and PCR (Fig. 2; Table 1) but also by deep sequencing, with no XMRV sequences detected out of 4 million deep sequencing reads (Fig. 5).

Coinciding with CIRM's goals, three of the floors planned for renovation will accommodate the collaborative work of top stem cell researcher and UCSB professor James Thompson, who was the first to isolate human embryonic cells in 1998; the new Mellichamp and Ruth Garland Chairs of bioengineering and molecular mechanisms, respectively; and the «Deep Sequencing Core,» which will house, among other things, a device that will help develop new genetic approaches to important cell biology inquiries.

Astronomers have detected candidates for pre-main sequence stars, very young stars just beginning their lives, as well as some T Tauri stars embedded deep within the nebula.

For example, by deep sequencing bacterial DNAs from diseased and healthy subjects, the inventors propose that features associated with certain microbiome compositional and / or functional features (e.g., the amount of certain bacteria and / or bacterial sequences corresponding to certain genetic pathways) can be used to predict the presence or absence of an autoimmune condition.

«The long, deep stretches of this style of yoga and its sequences are ideal for beginners and people who want to focus on flexibility, recovery from injury, body awareness, and relaxation.»

Yoga teachers love to incorporate this one into various sequences as a vital chest opener, core strengthener, as well as for a deep abdominal massage.

Utilizing pranayama, chant, and creative and intelligent sequencing, Tracy encourages students of all levels to practice intuitively and with deep respect for their individual yogic paths.

Speaking of Bisset, many filmgoers will remember The Deep primarily for that lengthy opening dive sequence and the following scene where she dries off in a boat with co-star Nick Nolte.

Rock's truths aren't as deep as those in Linklater's Before series, making room for a couple of Borat - strength scatalogical sequences that will make audiences wince and roar.

Tarsem Singh, a director known for his astonishing visuals more than his deep storytelling or compelling characters, continues his usual style by making an undoubtedly nice - looking flick but one which has only intermittent fun action sequences with mind - numbingly boring scenes in between.

It has everything a Star Wars fan could ask for: respectable to the lore, incredible visuals, ships and battle sequences, interesting characters (no they did not need deep back stories to be engaging), and the Peter Cushing and Darth Vader cameos were awesome.

«While the themes are deep, Black Panther is at the same time a visual joy to behold, with confident quirkiness (those aforementioned war rhinos), insane action sequences and special effects, and the glorious reveal of Wakanda, whose culture is steeped in African influences but which also offers a jaw - dropping look at what a city of the future could be... Let's not wait too long for a return trip.»

Miller's message is clear: if you need an action film with some deep, intricate plot to make the crazy death - defying sequences work for you, «Fury Road» is definitely not your kind movie.

Brice milks this (so to speak) for big laughs in a nude dance sequence — wearing convincing prosthetic dicks, Schwartzman and Scott are truly the genital equivalent of Arnold Schwarzenegger starring opposite Danny DeVito — but he also treats Alex's embarrassment with genuine empathy, briefly turning the film into a serious, affecting drama about deep - seated feelings of inadequacy.