Sentences with phrase «for endoderm»

As shown in Figure 4, all three germ layers developed in our cultures as shown by alphafetoprotein (AFP) for endoderm (Figure 4B), smooth muscle actin (SMA) and desmin for mesoderm (Figure 4D, E), glial fibrillary acid protein (GFAP) and SMI31 for ectoderm (Figure 4G, H).

Not exact matches

The researchers found that maternal gdf3 is required for Ndr1 and Ndr2 to signal at the levels necessary to properly induce the formation of mesoderm and endoderm cells in early zebrafish embryos.

«Through studies like that, we've been able to show how the main so - called germ layers for the embryo are formed — ectoderm, mesoderm, and endoderm.

For example, cells in the primitive streak can become either endoderm or one of two types of mesoderm.

But then Stainier and colleagues showed that the endoderm was also somehow responsible for the migration of the heart cell buds in the layer of cells above it, known as the mesoderm.

Additionally, ERCs were reported to be able to differentiate into, or become, cells from the three different germ layers (see the previous post on MSCs for more details): mesoderm (muscle, bone, fat, cartilage, and endothelial cells), ectoderm (neurons), and endoderm (liver, pancreas, and lung cells)(Meng et al., 2007; Patel et al., 2008).

In one of the experiments, for example, activation of a particular signal at the correct time elicits the appearance of the mesoderm, endoderm and ectoderm — the precursors of all cell types — with a spatial organization similar to that of an embryo.

When the ESCs had no Chd1 at all, the cells could not differentiate into all of the three germ layers (specifically, endoderm was not detected, and a preference was found for neuronal lineages).

«The use of endoderm for drug screening is a cornerstone of our drug discovery platform,» said John West, President & CEO of Novocell.

San Diego, CA (June 3, 2009)-- Novocell, Inc., a stem cell engineering company, today announced that it has received U.S. Patent No. 7,541,185 with method claims covering the use of endoderm cells derived from human embryonic stem cells (hESCs) for drug discovery.

The portfolio includes U.S. and foreign patents and applications directed to culture, expansion and scale - up of pluripotent stem cells and cell types derived therefrom, various differentiation processes for production of mesendoderm, definitive endoderm, PEC - 01, and endocrine precursors, as well as claims directed to the Company's Encaptra ® macro-encapsulation device platform.

Our technology enables the identification of potential endoderm differentiation factors, leading to potential discovery of new drugs & agents for research, and, ultimately, therapeutic use.»

Novocell scientists pioneered a differentiation process that successfully engineers hES cells into definitive and pancreatic endoderm cells necessary for pancreas formation.

«The most important application of this patent will be the use of our patented human endoderm cells to screen for agents that potentially affect endoderm differentiation, for example, to any of the important cells derived from endoderm such as lung, liver, intestine, pancreas, thymus, parathyroid and thyroid.»

Simultaneously, expression of markers for major developmental lineages, including trophectoderm (Cdx2), ectoderm (Fgf5), mesoderm (T) and endoderm (Gsc, Sox7 and Afp), was activated (Figure 4B, bottom panel).

For definitive endoderm differentiation, spontaneously formed EBs on day 6 were plated onto matrigel - coated dishes and maintained in a low concentration of fetal bovine serum (FBS) in combination with a high concentration of recombinant Activin A. Scale - like cells appeared (Figure 5A), which were positive for definitive endoderm markers such as Foxa2, Sox17 and GatFor definitive endoderm differentiation, spontaneously formed EBs on day 6 were plated onto matrigel - coated dishes and maintained in a low concentration of fetal bovine serum (FBS) in combination with a high concentration of recombinant Activin A. Scale - like cells appeared (Figure 5A), which were positive for definitive endoderm markers such as Foxa2, Sox17 and Gatfor definitive endoderm markers such as Foxa2, Sox17 and Gata4.

To induce mesoderm and endoderm lineages from iPS cells, cells were grown as embryoid bodies (EB) in DMEM / F12 with 10 % fetal bovine serum for 2 weeks.

That we have observed a direct contribution of ES cells to both visceral and parietal endoderm also has implications for canonical definitions of pluripotency.

Rational Bioprocess Design for Human Pluripotent Stem Cell Expansion and Endoderm Differentiation Based on Cellular Dynamics.

Differentiation into extraembryonic endoderm [21] or neural progenitors [33] are frequent early outcomes of spontaneous differentiation when human ES cells are cultured in the presence of a feeder cell layer, and it is interesting to speculate that the cells are being primed for these fates.

San Diego, CA (March 31, 2009)-- Novocell, Inc., a stem cell engineering company, today announced that it has received U.S. Patent 7,510,876 with claims covering human definitive endoderm cells, an essential cell for generating not only pancreatic type cells, which Novocell is developing for use as a cell therapy for diabetes, but also other endoderm lineage - derived tissues and organs such as lungs, intestine, liver, thymus and thyroid.

«This composition patent is a milestone achievement for Novocell and is the culmination of extensive research that opened the door to the endoderm lineage,» said Fred Middleton, Chairman & Acting CEO of Novocell.