The technology included a variety of expression systems, including the wheat germ cell free translation system
for eukaryotic gene expression, to take synthetic gene sequences and translate them into proteins.
Despite its power, RNAi is amazingly simple to perform in the roundworm C. elegans, an important model system
for eukaryotic gene function.
Not exact matches
To demonstrate that the mammalian cells did not acquire bacterial
genes by non-conjugational means —
for example, by engulfing the bacteria, as
eukaryotic cells sometimes do — Waters added an enzyme that digests DNA outside the cell membrane.
Researchers from the University of Seville at the Andalusian Centre
for Molecular Biology and Regenerative Medicine (Centro Andaluz de Biología Molecular y Medicina Regenerativa — Cabimer) have discovered that in
eukaryotic cells the proximity of the
genes to the nuclear pores, which are found in the nuclear membrane, contributes to maintaining the integrity of the genome.
You are what you eat: a
gene transfer ratchet could account
for bacterial
genes in
eukaryotic nuclear genomes.
«This is the only system I know about that is amenable
for structural work on how the cellular signal comes down to the chromatin and DNA and that can provide an answer about how a signal activates a
eukaryotic gene,» Panne says.
A system
for automated detection of homologs among the annotated
genes of several completely sequenced
eukaryotic genomes.