We have developed a novel «integrated» approach
for gene expression profiling analysis that combines both «unbiased» and «biased» profiling (22)[Fig. 5].
Drawing on data from the ORBIT study, a randomized, controlled trial of RA patients in the United Kingdom, researchers looked
for gene expression markers that would help predict responses to either TNFi drugs or the B - cell therapy rituximab, or both.
This is how he came to develop green fluorescent protein as a
reporter for gene expression, for which he was coawarded a 2008 Chemistry Nobel Prize.
A quest to find good
primers for gene expression analysis of Candida albicans from clinical samples — Gabriela C.Alonso — Journal of Microbiological Methods
Inspired by the knowledge - based analysis methods
developed for gene expression data analysis, we implemented methods for examining functionally - related SNPs as a group.
Four
strategies for gene expression are apparent during the parasites» life cycle: (i) housekeeping; (ii) host - related; (iii) strategy - specific related to invasion, asexual replication, and sexual development; and (iv) stage - specific.
Doing so opened up a vast bank of viable
samples for gene expression profiling, from which Dr. Golub and colleagues made some interesting findings.
Ryan Morin, a graduate student in the Marra lab, walked us through a summary of transcriptome sequencing and how it can be used not
only for gene expression, but also discovery of mutations and characterization of alternate splicing.
Multiple real - time PCR platforms are
available for gene expression studies, including Agilent Mx3005p and Mx3000p instruments, a Roche LightCycler 480 II with interchangeable 96 - and 384 - well modules, and a Roche LightCycler 1536 with an associated Innovadyne Screenmaker 96 +8 liquid handling workstation.
Mitobridge has used NanoString PlexSet
Reagents for gene expression, systematically interrogating disease - related changes in mitochondrial activity and translating unique insights into potential new treatments.
Trigonelline seems to aid in gene transcription, a required
step for gene expression, and reduces DNA damage at the cellular level.
By attaching a recently discovered «on - off»
switch for gene expression, taken from a gene that is turned on only in smooth muscle cells — which play a crucial role in cardiovascular disease — the researchers were able to restrict the action of the inserted genes to that specific cell type.
We don't know how well Martin Chalfie met these requirements, but we do know that he was allowed to use his wife's findings for his 1994 article «Green fluorescent protein as a marker
for gene expression», which is among the 20 most - cited papers in the field of molecular biology and genetics.
But by placing the gene under the control of a «promoter» — an on - off switch
for gene expression — from a gene (SM22a) that is expressed only in smooth muscle cells, Dr. Parmacek's team found that no matter which types of cells were infected, the gene was only «turned on» in smooth muscle cells.