Sentences with phrase «for genes of interest»

Maintained viable bacteria colonies and cultures for all necessary strains; conducted purification and extraction of genomic DNA; performed PCR for genes of interest; ran protein expression and activity assays.

Four replicate PCR experiments were conducted for all genes of interest.

Following the provided protocol, a portion of the RNA - containing lysate (5 — 10 µl) was hybridized at 54 degrees C overnight to RNA specific magnetic capture beads in the presence of blocking buffers, proteinase K and preordered mRNA probe sets specific for the genes of interest: HMOX1, Nrf2, Keap1, Bach1, and GAPDH.

The wells of a 96 - well optical reaction plate were loaded with a 20 - μl volume consisting of TaqMan 10 × PCR Master Mix, cDNA diluted in sterile H2O, and either a primer mix for the gene of interest or the housekeeping gene 18s.

For every run and for every gene of interest, several controls were utilized, including: 1) positive control B. burgdorferi RNA; 2) negative controls (a. no template control, b. unrelated template control); and 3) no RT control to check for DNA contamination.

Not exact matches

It is, as well, an antidote to Mississippi Burning, a dishonest, award - winning new film in which blacks wait patiently and fearfully in the background for deliverance by two white FBI agents, played by Gene Hackman and Willem Dafoe, who zealously bend the law in the interest of justice — a film one fears will have a profound effect on the way many Americans view their nation in the King Years («The Dream Dafoed,» as the Village Voice put it).

Wilson's interest for us, then, may lie in the fact that he articulates, at least in the «disenchanted» and secularistic tenor of his thought, what many educated people of our society increasingly suspect to be the case: matter and genes really are all that there is or ever will be.

During my time, more tools became available for plant breeders to transfer genes of interest and improve selection efficiency.

There has been considerable interest, for example, in a gene that produces low levels of an enzyme called monoamine oxidase A (MAOA) in the brain.

Because this imprinting affects hundreds of genes that are non-coding, including microRNAs and non-coding RNAs, it's a very interesting fine - tuning mechanism for the dosage of gene expression in the brain and elsewhere in the body.»

When looking for genes that might play important roles in the metabolism of healthy and cancerous liver cells, Wang and his colleagues became interested in a gene called SLC13A5, which produces a protein that transports citrate into cells.

As well as understanding the implications of finding this new variant of the botulism gene cluster in a non-clostridial species of gut bacterium, the team are interested in exploring how it might help in developing new treatments for diseases.

For a male fathering children with many mates, it's in the interest of his genes that the baby suck as much out of the mother as it can.

Another way to pass on the seemingly nonreproducing gay gene would be for a nonreproducing gay man to have an extra interest in seeing that the offspring of his siblings survive.

There are probably genes that code for being a nice guy, being a son - of - a-bitch; it's not a one - to - one relationship, but there are probably things that enhance aggressivity — well, things that enhance sociability — so that's interesting to know.

«The challenge has been how to direct certain therapies designed to manipulate genes of interest in specific cells without developing a specific drug carrier for each specific cell type.

Genomics - based discovery is key for identifying genes of potential interest to plant breeders and biotech seed developers.

One species, Glycine tomentella, was of particular interest because it has genes for resistance to soybean rust and to soybean cyst nematode, he said.

«This is an interesting example where mild mutations in a gene that is critical for normal development leads to phenotypic [observable] evolution,» said lead researcher Leif Andersson, a professor of functional genomics at Uppsala University, the Swedish University of Agricultural Sciences, and Texas A&M University.

Of particular interest are the emerging techniques for genomics and proteomics, which allow profiles of gene expression and protein synthesis to be produced and comparisons to be made between normal and abnormal cells, as well as between cells before and after exposure to medicines or toxic chemicalOf particular interest are the emerging techniques for genomics and proteomics, which allow profiles of gene expression and protein synthesis to be produced and comparisons to be made between normal and abnormal cells, as well as between cells before and after exposure to medicines or toxic chemicalof gene expression and protein synthesis to be produced and comparisons to be made between normal and abnormal cells, as well as between cells before and after exposure to medicines or toxic chemicals.

«We therefore looked for patient characteristics that would account for this result; next generation sequencing (NGS) of the genes of interest was performed on the archival tissue samples from the patients» primary tumours,» explained Ciardiello.

Epiviz implements multiple visualization methods for location - based data (such as genomic regions of interest) and feature - based data (such as gene expression), using interactive data visualization techniques not available in web - based genome browsers.

In the past few years there has been an explosion of interest in horse genetics, and last year a test was unveiled for a «speed gene».

Since the successful sequencing of the human genome, interest in the group of genes encoding proteins responsible for DNA remodeling has grown exponentially.

This form of interference with bacterial gene regulation is also of pharmaceutical interest as it is known that pathogenic bacteria can protect themselves against attack by the immune system and the effect of antibiotics by forming biofilms, for instance on the epithelium of the respiratory system.

Of particular interest was the group of genes responsible for temperature sensation, which also play roles in hair growth and fat storagOf particular interest was the group of genes responsible for temperature sensation, which also play roles in hair growth and fat storagof genes responsible for temperature sensation, which also play roles in hair growth and fat storage.

The genome of the grape used in Pinot Noir, first published in 2007, has revealed some interesting facts: For instance, the genes involved in regulating tannins, which help give wine its flavor, and for making resveratrol, the compound that supposedly gives wine its health - promoting properties, have become more potent over the centuriFor instance, the genes involved in regulating tannins, which help give wine its flavor, and for making resveratrol, the compound that supposedly gives wine its health - promoting properties, have become more potent over the centurifor making resveratrol, the compound that supposedly gives wine its health - promoting properties, have become more potent over the centuries.

The big thing then (as now), Ruvkun says, was for researchers to demonstrate that a gene of interest exists in a spectrum of different species — from roundworms and fruit flies to humans.

The chemical industry is interested in moving away from fossil fuels to bio-based products to reduce environmental impacts and to meet new regulations for sustainability, said Yong Wang, Voiland Distinguished Professor in the Gene and Linda Voiland School of Chemical Engineering and Bioengineering.

This under - expression was particularly interesting because although hundreds of genes have already been identified for their presumed roles in autistic disorders, each one alone can at best only explain 1 % of cases.

Stroud was interested in studying epigenetic changes, or chemical modifications that turn genes on and off, in DNA for the glucocorticoid receptor gene that regulates passage of cortisol from mother to fetus.

Cebrià highlights that «as there are genes which are similar to egr - 4 in other lineages — for instance the human species — , this function may be particularly interesting to understand the process of regeneration in metazoans.»

A second project, dubbed Eumorphia, will organize more than a dozen labs in nine countries to systematically screen new mouse mutants for interesting characteristics — a key step in determining the function of newly identified genes.

«Our results raise the interesting possibility that there's a genetic toolkit for this kind of behavior,» he says — a set of genes that have been used at several points in evolution to come up with similar behaviors.

In that sense, the female is acting in the best interest of her genes: A son will, on average, produce more grandchildren for her than would a daughter, because males have more opportunities to mate in a female - dominated population.

Thus µSCALE empties the contents of a single capillary onto a collector plate, where the DNA of the isolated cell can be sequenced and the gene variant responsible for the protein of interest can be identified.

He says HGS was getting «diminishing returns» from its investment in TIGR since Venter had steered his outfit into sequencing organisms of little medical importance, and into human genome sequencing, also of limited value for a company like HGS that is interested in genes as drug targets (not untranslated DNA that makes up most of the genome).

Beyond the interest these lateral gene transfers evoke in evolutionary biology, these results highlight the risk gene transfers could cause, in case GM - parasitoid wasps are produced, as genes artificially introduced into wasp species used for biological control could be transferred into the genomes of the targeted pests.

The discovery of the gene for DMD in 1987 fuelled interest in screening newborns for the disease, and state health authorities decided to test every newborn boy for the Duchenne gene as a matter of routine and without the explicit consent of the parents.

The team of Luis Lopez - Molina, professor at the Department of Botany and Plant Biology of UNIGE's Faculty of Science, Switzerland, has been interested for a long time in the mechanisms controlling germination, arguably the most critical decision in the life of a plant: «We have discovered that the genes involved in the synthesis of cutin, a waterproof substance, are important for the maintenance of dormancy in seeds of Arabidopsis thaliana, a small plant widely used as a model organism to study plant biology.

To modify these tagging vectors for your specific gene, the lab recommends designing homology arms with the modification of interest, ordering them from a gene synthesis company such as IDT or Genscript, and cloning them sequentially into the chosen acceptor vector.

A physician with a longstanding interest in gene therapy, Dr. High was formerly a Professor at the Perelman School of Medicine of the University of Pennsylvania, an Investigator of the Howard Hughes Medical Institute, and the Founding Director of the Center for Cellular and Molecular Therapeutics at The Children's Hospital of Philadelphia, a Center focused on developing novel cell and gene - based therapies for genetic disease.

If you are interested in a personalized cancer risk assessment and / or having genetic testing for a particular gene, you should have genetic counseling first to discuss possible test results as well as the benefits, risks and limitations of genetic testing.

For example, a researcher interested in cardiovascular disease may only want to examine the effect of a disrupted gene on blood pressure.

Regions and genes are functionally annotated to allow for rapid searching and visual identification of regions of interest with the option that user - supplied genomic phylogenies and metadata can be incorporated.

«The most surprising and interesting part is that the authors detected derived alleles in pathogen resistance genes,» commented Albert Zink, director of the Institute for Mummies and the Iceman in Bolzano, Italy.

It is also intended for post-docs and scientists already working in certain of these fields and who are interested in expanding their knowledge on the potential applications of these new techniques to their models or in neighbouring pathophysiological models of analysis of genes or diseases using genetically modified animals.

As a result, they are of great interest for fields such as biological imaging, medical diagnostics, drug delivery, and gene therapy.

His research interests involve pharmacogenomics for hemoglobinopathies and neuropsychiatric disorders, transcriptional regulation of human fetal globin genes and genotype - phenotype correlation in human genetic disorders.

Results of the evaluation of applications for Microinjectionist — Transgenics & Gene Targeting Facility (call of interest of 10/02/2014).