Sentences with phrase «for immunocytochemistry»

Following perfusions, eyes were either excised, cryoprotected, frozen and cut as described for immunocytochemistry above or the retina was removed intact as a whole - mount preparation.

For immunocytochemistry and neurite growth assays, a plating density of 5 × 104 cells / well of a 24 - well plate was used.

Right eyes were investigated by electron microscopy, left eyes were used for immunocytochemistry and fluorescence light microscopy.

Beginning in October 2017, Bio-Techne began to distribute Vector Laboratories products to complement the over 40,000 primary antibodies from Novus Biologicals ® validated for immunocytochemistry and immunohistochemistry.

Not exact matches

Pluripotent cells were cultured in mouse Noggin protein for 5 d and immunocytochemistry was used to detect retinal specific markers.

After editing, cells were analyzed for AcGFP1 expression and pluripotency via flow cytometry (Panel B) and immunocytochemistry (Panel C).

After, cells were analyzed by flow cytometry or immunocytochemistry for AcGFP1 and pluripotency markers.

Immunocytochemistry was performed essentially as described above for cells.

(B) Neurite length of GDF5 - treated (200 ng / ml daily for 72 h) SH - SY5Y cells at 72 h. (C — F) p - Smad1 / 5 levels as determined by (C, D) Western blots or (E, F) immunocytochemistry in SH - SY5Y cells 24 h after transfection with control miRNA or miR - 181a inhibitor.

To do this, SH - SY5Y cells were transfected with a miR - 181a inhibitor or a scrambled control, and then cultured for 24 h before p - Smad1 / 5 levels were examined by Western blotting (Figure 3C, D) and immunocytochemistry (Figure 3E, F) and quantified by densitometry.

The initial aim of the pathology investigation was to carefully examine the spinal cord using immunocytochemistry and electron microscopy, as well as classical techniques, to confirm the pathology previously reported and to look for new clues to the pathogenesis and aetiology using the more modern techniques.