Sentences with phrase «for immunofluorescence»

Cells used for immunofluorescence analysis were fixed immediately following overnight adherence to remove dead and non-viable cells and cells used for transcriptional analysis were cultured for 6 days prior to analysis.

For immunofluorescence, the cells were fixed in 4 % paraformaldehyde / PBS for 20 minutes, washed twice with PBS, and blocked with 4 % goat serum in PBS for 30 min, with all procedures performed at room temperature.

Frozen sections of lung and colon tissues from wild - type mice and Slc6a14 − / − mice were also used for immunofluorescence analysis of Slc6a14 protein expression with an antibody raised against a mouse peptide sequence [17].

The antibodies used in this study includes COX - 2 (Cayman Chemicals, Ann Arbor, MI, USA), STAT6 for Western blot (Cell Signaling Technology, Danvers, MA, USA), for immunofluorescence (IF)(BD Pharmingen, San Diego, CA, USA), for ChIP (Santa Cruz Biotechnology, Santa Cruz, CA, USA), phospho - STAT6 (Cell Signaling Technology), p300 for Western blot, (Upstate, Lake Placid, NY, USA), for ChIP (Santa Cruz Biotechnology), normal rabbit IgG (Santa Cruz Biotechnology), mouse immunoglobulin (Ig) G1, κ Ig (BD Pharmingen), glyceraldehyde -3-phosphate dehydrogenase (Advanced ImmunoChemical, Long Beach, CA, USA), Cy3 - conjugated AffiniPure Goat anti-mouse IgG (Jackson ImmunoResearch, West Grove, PA, USA) and PARP (Cell Signaling Technology).

For immunofluorescence staining, OCT - embedded 3 - and 5 - year - old testes were sectioned at 7 - µm thickness.

Antibodies used for immunofluorescence staining included YAP1 from Cell Signaling and TAZ from BD Biosciences.

(a)--(d) and (i)--(l), low magnification; (e)--(h) and (m)--(p), high magnification for immunofluorescence staining of Oct4.

Most importantly, surface staining is retained after fixation and permeabilization for immunofluorescence or other co-staining.

Not exact matches

«When you're doing immunofluorescence, for instance, if you're using the wrong fixation process or fixative you could really get the wrong results even with a great antibody,» he says.

In total, more than 500 proteins were analyzed with two well - established methods used in most laboratories for biological studies; immunofluorescence (IF) and fluorescence protein (FP) tagging.

For analysis of plug vascularization, CD34 (Novocastra, #NCL - END, 1:50) immunofluorescence staining was performed.

Choosing the correct secondary antibody is crucial for IHC and immunofluorescence.

Immunofluorescence microscopy for localization of Arabidopsis chloroplast proteins.

DropArray ™, a Wall-Less 96 - Well Plate for Uptake and Immunofluorescence Microscopy, Confirms CD22 Recycles.

All genes with Data reliability «Supported» in one or both of the two methods immunohistochemistry and immunofluorescence, or standard validation «Supported» for the Western blot application (assays using over-expression lysates not included) are classified as «Evidence at protein level».

The HepG2 cells were treated with ActD for 6 h, and the p53 expression level was examined by immunofluorescence imaging.

Bulk - cultured PC3 or PC3 - GFP cells, or clone - derived cultures were plated on glass coverslips and used in immunofluorescence staining for CD44, α2β1 integrin, and β - catenin using monoclonal antibodies and protocols described previously (27 — 29).

(C — D) Immunofluorescence staining for (C) TRA -1-60 and (D) TRA -1-81 on HUF1 cells.

Immunofluorescence for p63 and p73 was not performed.

To detect regulatory T cells, we co-stained our sections by immunofluorescence for FoxP3 and CD4 - expressing cells.

After binding, cells were washed once (carefully using a 200 μl pipette to remove the medium) with RPMI with 0.5 % BSA solution and adherent cells were analyzed by immunofluorescence microscopy or bright field microscopy for quantification.

(F — H) Immunofluorescence for cleaved caspase 3 (CC3, white arrows) in lesions.

(E) Immunofluorescence for pSTAT5.

An enzyme immunoassay (such as an ELISA test, short for enzyme - linked immunosorbent assay) or an immunofluorescence assay is the first step.

All cases were analysed for IgA anti-gliadin (AGA) by an ELISA test and those with positive results were tested for IgA anti-endomysium antibody (EMA) using immunofluorescence.

Immunofluorescence is a useful diagnostic measure when performed as immuno - histochemistry for antigen detection in tissues; however, it is typically performed on tissues obtained after death.

Immunofluorescence is sometimes used, for example.

Direct immunofluorescence for presence of antigen from smears of conjunctival, tonsillar and genital epithelium, and buffy coat.

Tags for this Online Resume: drug discovery, drug screening, FACS, immunofluorescence, western blotting, ELISA, cell based assay, MSD, cell toxicity assays, protain expression, target validation, cAMP assay, FRET, FLIPR

Key Highlight: • Handled flourochrome labeled antibody staining used for flowcytometry, cell proliferation assay, immunofluorescence cell staining and cytokine assays using ELISA.