Sentences with phrase «for immunohistochemical analysis»

MCF7, 231, and 468 lines and an ER - α — positive metastatic sample (COB) were implanted subcutaneously into mice, and xenografts were harvested at different time points; half was embedded in paraffin for immunohistochemical analysis, whereas half was dissociated for mammosphere culture.

The left hemibrain was fixed in 4 % paraformaldehyde for 48 h for immunohistochemical analysis, and the right hemibrain was frozen and stored at − 80 °C for biochemical analysis.

For immunohistochemical analyses, serial sections were stained with Alexa Fluor 594 - conjugated MECA - 79 and Hoechst 33342, and examined by confocal microscopy (LSM710; Zeiss, Germany).

Not exact matches

C, immunohistochemical analysis for KLF4 expression was conducted for primary breast tumors and brain metastatic tumors, and the numbers of nuclear or cytosolic staining were assessed under microscope.

Sections from all cases were subjected to stain with haematoxylin and eosin (H&E) for histopathological examination and immunohistochemical technique for Cyclin D1 in addition using flow cytometric technique to perform cell cycle analysis.

Immunohistochemical analysis of formaldehyde - fixed paraffin - embedded canine neuronal tissue sections, labelling GFAP with ab7260 at a dilution of 1/1000 incubated for 90 minutes at 25 °C.

As ER - α is considered a reliable biomarker in immunohistochemical analyses and is already used to guide endocrine treatment it would be a suitable treatment predictive marker for antiangiogenic therapies.