Sentences with phrase «for immunoprecipitation»

When an antibody to endogenous SUV39H1 was used for immunoprecipitation, MeCP2 was effectively coimmunoprecipitated; conversely, aHA antibodies to HA - tagged MeCP2 could immunoprecipitate SUV39H1 (Fig. 2G).»

Not exact matches

Called SIF - seq, for site - specific integration fluorescence - activated cell sorting followed by sequencing, this new technique complements existing genomic tools, such as ChIP - seq (chromatin immunoprecipitation followed by sequencing), and offers some additional benefits.

«fCLIP - seq stands for formaldehyde crosslinking, immunoprecipitation, and sequencing.

At ABclonal, we have nearly 100 existing chromatin immunoprecipitation (ChIP)- validated antibodies with a higher specificity than most commercial antibodies, which are used for a wide range of epigenetics studies, from those investigating developmental processes to those researching various diseases.

For example, the 2015 Nature scientific report, Important biological information uncovered in previously unaligned reads from chromatin immunoprecipitation experiments (ChIP - Seq) discusses how various computational tools can find new information that can assist in the construction of gene regulatory grids.

Importantly, this is one of the first reports applying Aβ immunoprecipitation followed by MALDI - MS analysis in CSF from a transgenic AD model, providing a robust, novel platform for the assessment of Aβ expression profiles in biological fluids of transgenic models.

Samples can be frozen at -80 °C for long - term storage, or be used for immediate Western blotting or immunoprecipitation.

For the coimmunoprecipitation experiments, HuH - 7 cells transfected with pcDNA - SRα / FLAG - Sp 1 were harvested and cell lysates were prepared using immunoprecipitation lysis buffer [20 mmol / L Tris - Cl (pH 7.5), 150 mmol / L NaCl, 10 % glycerol, and 1 % Triton X-100].

To evaluate whether loss of ABL kinases affected TAZ activity, we performed chromatin immunoprecipitation (ChIP) analysis using primers for TAZ targets identified by ChIP sequencing analysis (42).

(D) Cells transfected with either Keap1 or control siRNA were subjected to chromatin immunoprecipitation after treatment with either 10 µM HPP - 1014, 10 µM CoPP, or 1 µM HPP - 4382 for 6 hours.

For Western blot assay and immunoprecipitation analysis, CD4 + T cells were isolated from mice spleen on day 21 after transplantation using a Mouse CD4 + T Cell Isolation Kit (Miltenyi Biotec), and the procedures were performed as described previously (35).

A diverse portfolio of high - quality primary and secondary antibodies is available for use in cancer research, epigenetics, immunology, neuroscience, and stem cell research as well as validated for multiple applications, including flow cytometry, western blotting, and immunoprecipitation.

In the double mutant mice, the targeted gene is biotinylated, allowing for its specific immunoprecipitation and any bound proteins.

(D) Treatment with 1 mM 8 - Br - cAMP for four hours enhances Oct4 binding to genomic loci as determined by chromatin immunoprecipitation, followed by real - time PCR analysis at Nanog and fgf12 sites.

Abs used for Western blotting and immunoprecipitation were anti-phospho Syk, - phospho PLCγ1, - phospho PLCγ2, - ERK, - phospho p38, - p38, - Lyn (Cell Signaling Technology), anti-phospho SLP76 and - phospho LAT (BD Biosciences), anti-phospho tyrosine (clone pY20, Neomarkers), anti-phospho tyrosine (clone 4G10), - myc and - HA (Roche) and - FcRγ (Millipore).

Tags for this Online Resume: SDS - PAGE, plasmid prep, transfection, DNA, RNA, protein, PCR, plasmid construction, Immunoprecipitation, Immunostaining, IHC, IF, cell culture, microscopy