Finally, hydroxystilbamadine, also known as fluoro - gold, is a dye that can be used as a retrograde enhancer
for labelling neurons by injecting it in vivo.
Researchers have good methods
for labeling neurons based on where in the brain they developed, but the methods
for labeling neurons that develop at the same time are less precise.
Not exact matches
Forty such lines were tested
for their ability to increase aggression when their
labeled neurons were activated.
For this particular study, the research team created a small library consisting of different fly lines; in each line, a different set of specific
neurons was genetically
labeled and could be artificially activated, with each
neuron type secreting a different neuropeptide.
Such vectors have become critical tools
for delivering genes to treat disease or to
label neurons and their connective fibers with fluorescent colors to map out their locations.
To create the
labels, the scientists split a fluorescent molecule in half, one half
for the talking
neuron and one half
for the listening
neuron.
Using their own recently - developed genetic
labeling techniques
for tracking these cells in early development in mice, Taniguchi and his team observed that, like most
neurons, the cells remodeled their axonal organization through development.
Together, the present study suggests that GRPR +
neurons constitute a long - sought
labeled line
for itch sensation in the spinal cord.
Approach: Wu's group used Brainbow, a transgenic technique originally devised to map individual
neurons in the brain, to
label cells stochastically using gene cassettes that code
for four uniquely expressed fluorescent proteins.
A cortical layer 2/3
neuron at 21 days in vitro
labeled by ex utero electroporation with fluorescent marker
for spine visualization.
3D time - lapse of
neuron: Time - lapse image of mouse primary
neuron labeled with EGFP after co-culture with astrocyte
for 2 weeks.