Sentences with phrase «for oocyte maturation»

Not exact matches

Oocyte maturation and embryo development data were entered into a two - by - two contingency table, and Fisher's exact test was used to generate P - values in Prism version 5.02 for windows (GraphPad Software, Inc.).

However, whether human oocytes express the receptors for FSH and LH is controversial [9],, and it is known that EGF affects oocyte maturation through the EGF receptor (EGFR), which is expressed by cumulus cells rather than the oocyte [11].

We derived a protocol for paracrine / autocrine supplementation for in vitro maturation of cumulus - free oocytes that includes supplementation with BDNF, IGF - I, GDNF, leptin, FGF2 and estradiol as shown in Figure 2C.

In order to improve culture conditions for maturation of cumulus - free oocytes in vitro, we began by assaying the expression of 15 growth factors (BDNF, IGF - I, estradiol, GDNF, leptin, FGF1, FGF2, GM - CSF, EGF, TGF - α, TGF - β1; 2; 3, and ET - 1; 2) and 27 of their cognate receptors that have been shown to regulate oocyte maturation.

Following in vitro maturation, oocytes at metaphase II with the presence of a first polar body were activated using 10 µM calcium ionophore A23187 (Sigma - Aldrich) for 5 min followed by 2 mM DMAP (Sigma - Aldrich) for 4 h at 37 °C in 6 % CO2, 5 % O2 and 89 % N2.