Sentences with phrase «for phosphorylation»

Therefore, we conclude that FcεRI - induced activation of Syk is dependent on RhoH, which is essential for the phosphorylation of LAT and SLP - 76 adaptors as well as PLC phosphorylation and Ca2 + mobilization in mast cells.

Here, we provide evidence for a phosphorylation - based mechanism which regulates specific Oct4 homodimer conformations.

Previous studies have shown that the closely related Oct1 is dynamically phosphorylated [38], and site - directed mutagenesis of potential phosphorylation sites in Oct4 indicated a potential role for phosphorylation in regulating homodimer complex formation [18].

Binding of raptor to mTOR is necessary for phosphorylation of 4E - BP1 and p70S6K (Fig. 7).

Yang and co-author Chunru Lin, Ph.D., an assistant professor in the same department, confirmed four sites for phosphorylation, a process that turns protein enzymes off and on, impacting key cellular functions.

Not exact matches

For example, cancer cells tend to use glycolysis in the cell cytoplasm in their metabolism, an ancient system, whereas healthy cells use oxidation - phosphorylation.

The next logical step for me work-wise was at the University of California, Riverside, just outside Los Angeles, where I could investigate the physiological phosphorylation of the proteins I had learned to purify.

One way for that to happen is through changes in gene expression, but changes in phosphorylation are equally effective,» explains Beltrao.

«We also showed that TCL1 inhibits a mitochondrial enzyme required for in oxidative phosphorylation, leading to a reduction in oxygen consumption of the cells,» co-first author Shiho Aizawa explains.

Hund's team identified a phosphorylation site (Serine 571) on the voltage-gated sodium channel targeted by CaM kinase II that serves as a switch for inappropriate «late» sodium influx.

Specifically, they looked at eIF2alpha phosphorylation and the regulation of protein synthesis in the mice's hippocampus region — the part of the brain responsible for the retrieval of old memories and the encoding of new ones.

Thus, the team has demonstrated a novel, positive role for PKR activation and eIF2 - alpha phosphorylation in human globin RNA splicing, in contrast to the long - standing negative role of this intracellular stress response in protein synthesis.

«Pin1 changes protein shape through proline - directed phosphorylation, which is a major control mechanism for disease,» explains co-senior author Kun Ping Lu, MD, PhD, Director of Translational Therapeutics in the Cancer Research Institute at BIDMC and Professor of Medicine at Harvard Medical School who co-discovered the enzyme in 1996.

A role for casein kinase II phosphorylation in the regulation of IRF - 1 transcriptional activity.

This discovery provided the physiological basis for subsequent development of fMRI and caused researchers to reconsider the dogma that brain uses oxidative phosphorylation exclusively to fuel its functional activities.

Huda N, Tanaka H, Mendonca MS, Gilley D. DNA damage - induced phosphorylation of TRF2 is required for the fast pathway of DNA double - strand break repair.

Interplay between Sumoylation and Phosphorylation for Protection against α - Synuclein Inclusions.

We have used systematic molecular bioengineering of the meningococcal LPS through deletion of enzymes involved in LPS biosynthesis in combination with induction of LPS modifying enzymes (for example the deacylase PagL) to yield a variety of novel LPS mutant strains with changes in both lipid A acylation and phosphorylation.

VEGF internalization is not required for VEGFR - 2 phosphorylation in bioengineered surfaces with covalently linked VEGF.

van der Lee R, Szklarczyk R, Smeitink J, Smeets HJ, Huynen MA, Vogel R. Transcriptome analysis of complex I - deficient patients reveals distinct expression programs for subunits and assembly factors of the oxidative phosphorylation system.

Gene - specific requirement for P - TEFb activity and RNA polymerase II phosphorylation within the p53 transcriptional program.

Ipl1p - dependent phosphorylation of Mad3p is required for the spindle checkpoint response to lack of tension at kinetochores.

Searching for those substrates and trying to unravel how phosphorylation affects their activity has kept Hunter and his lab busy for more than three decades.

Phosphorylation of a borealin dimerization domain is required for proper chromosome segregation.

The effect of insulin on phosphorylation of Akt protein was measured by Western blot in enhanced hiPS - HEP cells from C18 on Day 12 post-thawing (n = 3 for the [+] condition; n = 1 for the -LSB----RSB- control).

Thirteen of these genes provide instructions for making enzymes involved in oxidative phosphorylation.

We previously demonstrated that J147 up - regulates the BDNF pathway in huAPP / PS1 mice following seven months of treatment, and here we show that even when administered at a stage when pathology is advanced, J147 can significantly increase two factors critical for memory formation: CREB phosphorylation and BDNF expression (Figure 3H and 3C, respectively).

8.30 Hana Paculová CDK12 connects transcriptional regulation to DNA damage repair pathway 8.55 Karol Kaiser Wnt secretion during nervous system development 9.20 Simona Hankeová The role of Notch signaling in vasculogenesis 10.00 Coffee break 10.30 Tomáš Doležal Extracellular adenosine regulates complex host - pathogen interactions through the energy release for the immune response 10.55 Ondřej Bernatík and Igor Červenka Regulation of diverse function of Dvl by phosphorylation 11.20 Jan Ryneš Dissection of Axin interactome: rational approach for control of signaling cascades via intervention with specific protein - protein interactions 12.05 Targeting opportunities: discussion & sum - up 13.00 Lunch and departure

PhoB has a well characterized DNA binding domain whose affinity for DNA is strongly enhanced by phosphorylation [17], [18], [19].

Modifications for Mitochondrial MEDEVAC However, when they then went on to attempt to use their system to introduce WT mitochondrial tRNAs into MELAS and MERRF cybrids — and thus rescue oxidative phosphorylation (OXPHOS)-- Teitell's group encountered a series of hurdles requiring additional modification of their constructs.

Heterogeneity and low abundance make protein phosphorylation analysis difficult, particularly for mammalian tissue samples.

Although protein phosphorylation is known to be important for many synaptic processes and in disease, little is known about global phosphorylation of synaptic proteins.

For this, the cells use the tricarboxylic acid (TCA) cycle, also known as the Krebs cycle and as oxidative phosphorylation.

Importantly, stimulation of the crosslinking of CD4ζ chimeric receptors led to tyrosine phosphorylation as would be expected for a fully functional chimeric receptor.

Hunter is known for his 1979 discovery of a mechanism called tyrosine phosphorylation, which is a molecular switch that turns normal cells cancerous.

For example, ILPs induce the phosphorylation of AKT, which in turn phosphorylates the negative growth regulator Forkhead Box O (FoxO).

Spleen CD4 + T cells were harvested from mice 21 d after transplantation and cultured or not with VPA for 24 h. Western blot assay showed that VPA attenuated the phosphorylation level of Akt in a dose - dependent manner (Fig. 4F).

(H) Spleen CD4 + T cells harvested from VPA recipients and control mice on day 21 were analyzed by Western blot assay for the expression and phosphorylation level of Akt and downstream target proteins.

We found that ~ 90 % knockdown of ABL1 alone resulted in enhanced ABL2 expression and did not produce a significant decrease in the phosphorylation of CrkL, a reporter for the activation state of the ABL kinases (Fig. 2K), and did not inhibit metastasis (Fig. 2, L and M).

To examine whether lethal toxin modulated JAK2 and / or STAT3 signaling, we next treated MNK - 3 cells with lethal toxin for 2 hr, stimulated with IL - 23 for the indicated time and then examined JAK2 and STAT3 phosphorylation by western blot analyses.

For his Ph.D, he characterized the evolutionary dynamics of phosphorylation sites for functional phospho - proteomics and to understand the evolution of phosphorylation - based cell signaliFor his Ph.D, he characterized the evolutionary dynamics of phosphorylation sites for functional phospho - proteomics and to understand the evolution of phosphorylation - based cell signalifor functional phospho - proteomics and to understand the evolution of phosphorylation - based cell signaling.

Lin28a bound to and enhanced the translation of mRNAs for several metabolic enzymes, thereby increasing glycolysis and oxidative phosphorylation (OxPhos).

This activation partly occurs through, for example, phosphorylation of GSK - 3 which prevents its own inhibition of SREBP1c.

Aldo - keto reductase 1C2 is essential for 1 - nitropyrene's but not for benzo [a] pyrene's induction of p53 phosphorylation and apoptosis.

Phos - tag analysis of Rab10 phosphorylation by LRRK2: a powerful assay for assessing kinase function and inhibitors

The most common mechanism for constitutive activation and phosphorylation of STAT factors is the dysregulation of tyrosine kinases.

The model was constructed to predict the receptor phosphorylation levels in a cell under specific conditions for the immediate - to - short (minute - to - hours) response durations.

The p38 - mediated p53 phosphorylation at Ser15 and subsequent p53 induction has been reported to be responsible for apoptosis induced by nitric oxide, hypoxic conditions, DNA - damaging agents, 1 - nitropyrene, and benzo [a] pyrene [30 - 32].

Phosphorylation of mTOR is increased in response to leucine [20], an excellent substrate for SLC6A14; as such, our findings that mTOR phosphorylation is markedly decreased in Slc6a14 − / − tumours indicate that the tumours suffer from amino acPhosphorylation of mTOR is increased in response to leucine [20], an excellent substrate for SLC6A14; as such, our findings that mTOR phosphorylation is markedly decreased in Slc6a14 − / − tumours indicate that the tumours suffer from amino acphosphorylation is markedly decreased in Slc6a14 − / − tumours indicate that the tumours suffer from amino acid deprivation.

Phosphorylation of AKT decreased after treatment with ActD for 10 minutes in the 293, 293T, and Hepa - 1c1c7 cells, but still increased after treatment with ActD (10 and 30 nM) for 10 minutes in the HepG2 cells.