Over the last 35 years the soil bacterium Agrobacterium tumefaciens has been the workhorse tool
for plant genome engineering.
Not exact matches
The platform is used
for everything from analyzing seismic data to find promising places to drill
for oil to looking at
plant genomes in search of new hybrids.
Selection
for this suite of traits leaves a mark on the
plant's
genome by the reduction of genetic diversity in the areas that code
for these traits.
Thinking that more hemoglobin might help
plants better survive a low - oxygen environment, Leif Bülow and colleagues at the Center
for Chemistry and Chemical Engineering in Lund, Sweden, inserted the Vitreoscilla bacterium's hemoglobin gene into the tobacco
genome.
The resulting backcrossed
plants and information about their
genomes, «shows a way forward
for improving chickpeas and many other crops too,» says von Wettberg, a professor in UVM's Department of
Plant and Soil Science.
The new method by Garaycochea and colleagues will enable researchers to strategically analyze this whole -
genome sequence data and assemble the chloroplast
genome for their
plant of interest.
As
for the identities of Mendel's other four genes, Weeden says he expects them to be revealed in the next few years as more
plant genomes give up their sequences.
For a
plant to create reproductive cells, it must first erase a key code, a series of tags attached to DNA across the
genome known as epigenetic marks.
«Studying the
genome of water - efficient
plants may also provide insights into a
plant's ability to use slightly saline water and maintain growth under higher temperature and lower clean water availability,» said Jerry Tuskan, coauthor and chief executive officer of the Center
for Bioenergy Innovation led by ORNL.
Also online, an interactive feature including informational graphics, video clips, and more, as well as accompanying Web resources, explore how
plant genome research is contributing to our understanding of
plant biology and evolution and leading to tangible benefits
for society.
He is typical of
plant -
genome scientists, whose work often combines a passion
for understanding their subjects» inner workings with a belief that their findings will help counter some of humanity's most urgent problems: hunger and malnutrition, the demand
for renewable energy, and climate change.
Chloroplasts evolve slowly, so researchers use next - generation sequencing to look
for subtle differences in their
genomes to determine how two
plants from different populations might be related.
The USDA is negotiating a deal between the US and Britain on sharing data, whereby wheat information would be deposited in the National Agricultural Library's
plant genome database in Maryland, and British scientists would receive software tools
for interrogating the database.
Uribe - Convers and colleagues tested this approach by amplifying chloroplast
genomes for 30 species across flowering
plants.
To use CallHap, researchers must first obtain a reference
genome sequence
for their target species, either from previously published work or by sequencing the DNA of a single
plant.
The shared
genome map cries out
for shared research, but the competitive advantage to
plant breeders has apparently ruled out cooperation within the European Union.
«
Genome editing technology is advancing rapidly; therefore it is timely to review the regulatory system for plant breeding by genome editing,» says Dr.
Genome editing technology is advancing rapidly; therefore it is timely to review the regulatory system
for plant breeding by
genome editing,» says Dr.
genome editing,» says Dr. Ishii.
Over the longer term, the identification of the coffee tree
genome sequence opens up new possibilities
for varietal improvement, knowledge of the specific functions of the genes (in particular those specific to coffee trees), the possibility of transferring results to other species, and refining diagnostic tools
for the function of the
plant.
Researchers looked
for regions of each
plant's
genome that showed unusual patterns of variation consistent with past selective breeding acting on the VRN - D4 gene.
When they inserted those genes into crop
genomes, through a technique called recombinant DNA, the
plants were able to produce the protein
for bug resistance on their own, eliminating the need to spray insecticide.
Dr Chatterjee's research uses an approach called TILLING (Targeting Induced Lesions In The
Genome), an established non-GM method
for creating and discovering new traits in
plants.
The authors then combined the new
genomes with thousands of publicly available
genomes that represent the major groups of
plant - associated bacteria, and included bacteria from multiple
plant and non-
plant environments, such as the human gut,
for comparison.
As scientists race to decode
genomes — not just of humans but of bacteria, yeast, chimps, dogs, whales and
plants — the number of DNA sequences available
for analysis has grown 40,000-fold in the past 20 years, providing unprecedented insight into billions of years of species evolution.
(Ill - fitting because humans have been indirectly, and much less precisely, modifying
plant and animal
genomes for thousands of years via selective breeding, and evolution has been doing it
for as long as there has been life on Earth.)
«Each of these 10
genomes encodes
for a unique composition of CAZymes — and the wider assortment helps formulate enzyme cocktails better suited
for different types of
plant biomass to efficiently convert them into biofuels,» Grigoriev said.
On 20 December 2013, a paper by the Amborella
Genome Sequencing Project that includes a full description of the analyses performed by the project, as well as implications
for flowering
plant research, will be published in the journal Science.
Many crop
plants contain
genomes that are hundreds or tens of thousands times larger: wheat,
for example, has an estimated 15 billion base pairs.
With an entire
plant genome to work from, biologists can now probe the genes responsible
for such basic
plant activities as budding, blooming, sleeping and seeding.
«Because of Amborella's pivotal phylogenetic position, it is an evolutionary reference
genome that allows us to better understand
genome changes in those flowering
plants that evolved later, including
genome evolution of our many crop
plants — hence, it will be essential
for crop improvement,» stressed Doug Soltis of the University of Florida.
«These archaeological remains provided a unique opportunity
for us to finally sequence a Chalcolithic
plant genome.
A genetic analysis, which included sequencing the entire
genome of Cephalotus, found strong evidence that during their evolution into carnivores, each of these
plants co-opted many of the same ancient proteins to create enzymes
for digesting prey.
They have made possible wholesale scans that turn up new gene modifications and variations, and the same should prove true
for the new human gene arrays, says Joseph Ecker, a
plant scientist at the Salk Institute
for Biological Studies in La Jolla, California, who has helped pioneer whole -
genome chips
for Arabidopsis.
First, samples of leaves from these
plants are collected
for in vitro cultures to isolate the fungi; then the DNA and RNA of fungi are extracted to sequence them and, through bioinformatic analysis, the researcher can determine the expression, the presence or absence of genes in the
genomes of a species against each other.
«Setaria viridis is a small
plant that can produce a lot of seeds faster, has a pretty simple
genome and can serve as a model
for research.»
Whole -
genome chips exist already
for five other organisms: the yeast Saccharomyces cerevisiae, the nematode Caenorhabditis elegans, the fruit fly Drosophila melanogaster, a
plant called Arabidopsis tumefaciens, and the gut bacterium Escherichia coli.
For the individual
plant, those possibilities depend on the
genome it was born with.
Still - unfunded plan would start with all
plants, animals, and other eukaryotes — some 1.5 million species —
for the cost of the original human
genome project
«The animals also do not have the genes
for plant - digesting enzymes in their own
genome.
As more such species are sequenced, he says, «everyone will be using these»
genomes to find out which genes are responsible
for particular functions, especially
for plant pathogens such as Fusarium, which causes ear rot in corn and puts out mycotoxins in animal feed.
The discovery, the product of a multiyear effort to provide a high - quality full
genome map of the oil palm
plant and to scour the sequence
for genes of importance to both science and industry, has major implications
for agriculture and the environment.
While looking
for ways to make tomatoes and other crop
plants more productive, BTI Assistant Professor Joyce Van Eck and former postdoctoral scientist Sarika Gupta developed a better method
for «transforming» a tomato — a process that involves inserting DNA into the tomato
genome and growing a new
plant.
At just under 600,000 bases, M. genitalium sports the smallest known
genome for a free - living organism, and Venter hoped that an artificial
genome could be modified to turn the bacterium into a living chemical - manufacturing
plant.
Of the three cell wall components, lignin is the most troublesome
for the biofuel industry because it limits the extraction of cellulose and hemicellulose
for biofuel production, according to Siobhan Brady, an assistant professor in the Department of
Plant Biology and
Genome Center at UC Davis and a co-author of the study.
the new information enabled the researchers to clarify aspects of the barley
genome that are important in the context of
genome evolution and
for practical use of
genome knowledge by
plant breeders and basic researchers — namely, the locations of gene - rich regions including some that have low recombination
Scientists can readily obtain extensive genetic information
for any
plant species of interest, at a relatively low cost, rapidly accelerating the pace of
genome sequencing.
Plastids have their own
genome and are responsible
for harvesting energy from light in photosynthetic
plants and algae.
Nosil and his colleagues sequenced the
genomes of dozens of individuals of each ecotype from multiple hillsides to assess the genetic differences that arose to make them specialized
for their particular host
plant.
His development of a method
for stable transformation of land
plant chloroplast
genomes, once thought to be impossible, established the field of chloroplast
genome engineering in higher
plants and has led to an explosion of research concerning the chloroplast
genome's role in photosynthesis, functional analysis of plastid genes by reverse genetics, and mechanisms of plastid gene regulation.
Support
for the undergraduate intern program comes from the National Science Foundation through the
Plant Genome Research Program grant awarded to Professor Georg Jander of BTI and Professor Jian Hua of Cornell University.
Researchers have sequenced the
genome of the whitefly (Bemisia tabici), an invasive insect responsible
for spreading
plant viruses worldwide, causing billions of dollars in crop losses each year.