Sentences with phrase «for pyrosequencing»
Purified amplicon DNAs were quantified using Quant - iT PicoGreen kit (cat # P7589, Invitrogen, Carlsbad, CA) and pooled for pyrosequencing.
https://bmcmicrobiol.biomedcentral.com/ Not exact matches
The recent introduction of the pyrosequencing - based 454 sequencing platform (454 Life Sciences, Branford, CT) offers a very promising sequencing technology alternative for incorporation in WGS.
The facility's infrastructure includes a pyrosequencing genetic analyser (PyroMark ID MA) capable of quick and efficient SNP analysis of as many as 480 samples per day, in addition to all the necessary equipment for a more conventional microsatellite genotypic analysis.
A Sanger / pyrosequencing hybrid approach for the generation of high - quality draft assemblies of...
A Sanger / pyrosequencing hybrid approach for the generation of high - quality draft assemblies of marine microbial genomes.
Mostafa Ronaghi, Ph.D., Stanford Genome Technology Center, Palo Alto, Calif. $ 1.8 million (3 years) «Pyrosequencing Array for DNA Sequencing» The principal investigator of this team is an inventor of pyrosequencing, which uses unmodified nucleotides and polymerases to synthesize DNA and a firefly enzyme to generate a chemiluminPyrosequencing Array for DNA Sequencing» The principal investigator of this team is an inventor of pyrosequencing, which uses unmodified nucleotides and polymerases to synthesize DNA and a firefly enzyme to generate a chemiluminpyrosequencing, which uses unmodified nucleotides and polymerases to synthesize DNA and a firefly enzyme to generate a chemiluminescent signal.
These studies, however, were based on comparing Life Technologies» SOLiD and Illumina's GAII with pyrosequencing or Sanger sequencing [8], [9], [10], the costs of which are prohibitive for whole - genome sequencing studies in mammals.
Computer simulations by Liu et al. [43], Wang et al. [41], and Sundquist et al. [47] have indicated that pyrosequencing tags from different regions of the gene will vary in their utility for the distinct tasks of revealing microbial diversity and performing taxonomic classification, both of which contribute to making informative comparisons between complex microbial communities.
B. Correlation of the fold differences between CPA and control obtained by Q - MeDIP and by microarray for the 20 amplicons analyzed in A. C. DNA methylation differences (%) between the groups in the GRM5, ITGB6, OR13C8 and IL - 31 genes validated by pyrosequencing.