Sentences with phrase «for stable expression»

However, these studies are limited by the requirement for stable expression of an exogenous gene.

Not exact matches

It's not that methylation doesn't do anything at all — in fact, the researchers found that it is primarily associated with genes that serve crucial functions for workers and queens alike, suggesting that DNA methylation might contribute to the stable expression of so - called household genes.

And in 1995 it reported creating petunias with more stable gene expression — and a vivid orange color — fit for commercial breeding.

Previous work has given insights into the mechanistic pathways leading to small molecule sequestration in membrane - free coacervate protocells; successfully demonstrated the use of liquid - liquid phase separated micro compartments for protein expression using cell free expression systems; exploited microfluidics for the high throughput formation of stable, monodisperse microdroplets; and integrated two contrasting modes of protocell construction to produce a novel hybrid model based on fatty acid membrane - bound coacervate micro-droplets.

Indeed this sort of model has been used to explain the existence of a stable attractor and associated lineage primed states in EML cells, a haematopoietic progenitor cell line [54], and as the basis for heterogeneity in Nanog expression in ES cells [11].

Dr. Mikhail Liskovykh an expert in Human Artificial Chromosomes (HAC) construction and transfer, including the assembly of centromere arrays, generating conditionally stable HACs, and cloning complete genetic loci for expression in human cells.

Timelines for the experiment (s): 2 - 16 weeks depending on the expression system (insect or mammalian cells) and method (transient or stable).

However, like most vertebrate cell lines, target sequences are inserted randomly and stable mutation and expression of the transcript can not be controlled for.

In contrast, the stable HB1.F3.Lenti - H2IgG cell line showed a shift in green fluorescence intensity for the entire cell population, albeit at lower levels than with the transient expression methods.

In contrast to this relatively stable expression, when juvenile birds sang, FoxP2 was acutely down - regulated in area X relative to the surrounding striatum (Fig. 3), similar to what we previously reported for adult birds [21].

Timelines for the experiment (s): 2 - 16 weeks depending on the expression system (insect or mammalian cells) and method (transient or stable), with 3 months lead time.

Trastuzumab heavy and light chains were reformatted into a bicistronic expression cassette and cloned into adenoviral and lentiviral vectors for transient and stable expression, respectively.

Since to me (and many scientists, although some wanted a lot more corroborative evidence, which they've also gotten) it makes absolutely no sense to presume that the earth would just go about its merry way and keep the climate nice and relatively stable for us (though this rare actual climate scientist pseudo skeptic seems to think it would, based upon some non scientific belief — see second half of this piece), when the earth changes climate easily as it is, climate is ultimately an expression of energy, it is stabilized (right now) by the oceans and ice sheets, and increasing the number of long term thermal radiation / heat energy absorbing and re radiating molecules to levels not seen on earth in several million years would add an enormous influx of energy to the lower atmosphere earth system, which would mildly warm the air and increasingly transfer energy to the earth over time, which in turn would start to alter those stabilizing systems (and which, with increasing ocean energy retention and accelerating polar ice sheet melting at both ends of the globe, is exactly what we've been seeing) and start to reinforce the same process until a new stases would be reached well after the atmospheric levels of ghg has stabilized.

Tags for this Online Resume: Lentiviral Retroviral shRNA RNA knockdown screens, Flow Cytometry, RTPCR, Immunopreciptations, Reporter assays, Cell based Assays, ELISA, Migration, Stable and Transient Transfections, Genomic and Proteomic Studies, Cloning, Transient siRNA knockdowns, Phenotyping Mammalian cell lines, Gene Overexpression, Subcloning to expression Vectors, Construct Stable Inducible Cell lines, Protein Kinase Assay, Pulse Chase Experiments, Transfection, Transduction, Transformation, Polyoma virus DNA tumor virus replication, Research Scientist, Cell Biology Protein Biochemistry Genetics Molecular Bioliogy Oncology Virology, FACS