Several highly conserved residues in SERT have been shown to be critical
for its subcellular localization, and mutation of these sites may contribute to both obsessive - compulsive disorder (OCD) and autism.
In the first step, stainings in different cell lines with the same antibody are reviewed and the results are compared with available protein / gene characterization data
for subcellular location.
Deputy Editor Steven Carr of The Broad Institute will talk about new approaches
for subcellular localization of proteins and their interaction partners with high spatial resolution.
Her doctoral research was conducted in the laboratory of Erik Lee Snapp and focused on optimizing fluorescent proteins
for subcellular environment.
Not exact matches
For them, that exercise might seem equivalent to pondering whether those individual
subcellular constituents are alive on their own.
They can also track standard molecular markers
for mitochondria, nuclei, and other
subcellular structures.
«By offering the distinct advantage of
subcellular resolution and avoiding the undesirable influence of fluorescent dyes, we believe our technique can complement FDG in clinical PET imaging
for visualizing glucose uptake activity at the cellular level,» says the lead author Fanghao Hu, a Ph.D. candidate in chemistry.
Importantly, they observed a significant disruption of the assembly of the axon initial segment (AIS), a
subcellular structure in the proximal part of the axon important
for clustering of ion channels, and thus,
for neurons to fire action potentials.
Korenberg's team is developing a 3D coordinate system to align various types of neuroimaging data in the macaque brain, from whole - brain MRI connectivity to single - cell confocal data and,
for some areas,
subcellular resolution with electron microscopy.
a publically available portal
for researchers in life sciences and provides access to protein profiling data in 46 normal tissues, 20 cancer tissues, 46 cell lines and on a
subcellular level.
This technique allows us to capture images of biological processes,
for instance to trace individual tumor cells in mice
for several weeks at
subcellular resolution.
A major restructuring of the knowledge portal has been done with the release of four separate sub-atlases; Normal tissue atlas,
Subcellular atlas, Cell line atlas and Cancer atlas, and RNA transcript data has been added
for a majority of the tissues in the normal tissue atlas and the cells in the cell line atlas.
Our technological expertise ranges from the most fundamental approaches to study membrane transport in lymphocytes and dendritic cells (
subcellular compartmentalization, intravital microscopy, phagosomal functions), the systematic analysis of gene expression and it regulation (RNAseq, Chip Seq, proteomics) and physiological and pathological immune responses (mouse models
for cancer immunity, immunomodulation / vaccination, human clinical studies in cancer).
Distinct
Subcellular Trafficking Resulting from Monomeric vs Multimeric Targeting to Endothelial ICAM - 1: Implications
for Drug Delivery.
Lysates from these two cell populations were fractionated on the basis of the
subcellular localization using the ProteoExtract Kit, and 40 μg of proteins was loaded
for Western blotting.
In the second step, all antibodies targeting the same protein are taken in consideration
for final annotation of the
subcellular localization.
For each cell line and antibody, the intensity,
subcellular location and single - cell variations (SCV) of the staining are described.
The table below lists the
subcellular locations used
for annotation, links to the cell structure dictionary entry and corresponding GO terms.
The Matrigel - based chemoinvasion assay is a powerful technique
for studying invadopodia's molecular composition and organization at the
subcellular level.
«The proteomics field is now entering a third generation phase where large - scale protein analysis will provide unique quantifiable data on a
subcellular level «says one of the participants in the EU funded PROSPECTS project Dr. Emma Lundberg from the Science
for Life Laboratory Stockholm.
The core facility is a two - photon in vivo imaging platform developed at the Nonlinear bioimaging laboratory, a technique that allows
for non-invasive structural and functional measurements in small animal models at different scales: from macroscopic imaging of the brain morphology to highly resolved microscopy of neuron populations, single neurons, and even
subcellular structures.
Distinguish between models
for trafficking to the lysosome by fusing Medium - FT to LAMP - 2A, a lysosome - associated membrane protein, and following its
subcellular localization overtime (Subach et al..)
A novel algorithm
for automatic reconstruction of
subcellular motion.
Based on the resource of more than 47,000 antibodies and a panel of > 20 different human cell lines, this facility has a unique position to investigate
subcellular spatial proteomics
for human and rodent biology.
The group also found that mNeonGreen works well
for GFP's typical applications, from tagging proteins to labelling
subcellular compartments, and that the protein is as stable against photodegradation as GFP.
The combination allows
for the study of 3D
subcellular processes in their native multicellular environments at high spatiotemporal (space and time) resolution.
With the
subcellular location restricting a protein's possible function, this method should be a useful tool
for the systematic analysis of genome data and is available via a server on the world wide web.
For three possible
subcellular locations in prokaryotic organisms a prediction accuracy of 81 % can be achieved.
Our results are the most accurate
subcellular localization classifications to date, and demonstrate the usefulness of deep learning
for high throughput microscopy.
Mitochondria —
subcellular organelles that generate chemical energy to power cellular processes and also serve as sites
for numerous metabolic processes and reactions
METHODS: We treated 3T3 - L1 adipocytes with 2.5 mmol / l R (+) alpha - lipoic acid
for 2 to 60 min, followed by assays of: 2 - deoxyglucose uptake; glucose transporter 1 and 4 (GLUT1 and GLUT4)
subcellular localization; tyrosine phosphorylation of the insulin receptor or of the insulin receptor substrate - 1 in cell lysates; association of phosphatidylinositol 3 - kinase activity with immunoprecipitates of proteins containing phosphotyrosine or of insulin receptor substrate - 1 using a in vitro kinase assay; association of the p85 subunit of phosphatidylinositol 3 - kinase with phosphotyrosine proteins or with insulin receptor substrate - 1; and in vitro activity of immunoprecipitated Akt1.