We added the [cost of the] parts
for the hybridization of the car.
Evaluating Options
for the Hybridization of Concentrated Solar Power (CSP) and Biomass Through Gasification in the Brazilian Semi-arid
Not exact matches
A theological vision is necessary
for Evangelicals to shape the intuitions we will need to face the challenges
of the future — whether they are ethno - nationalism, artificial intelligence, or the blurring
of what humanity itself is through cloning and
hybridization.
The SureSeq mix increases the success and accuracy
of hybridization - based NGS
for FFPE samples by repairing damage such as nicks and gaps, oxidized bases, blocked 3» ends, and deamination
of cytosine to uracil.
Among other adjustments that could help double fuel economy are turbocharging with smaller, more efficient engines that produce the same level
of power; advanced heat management and cooling systems, which reuse the heat produced in the engine
for energy; weight reduction, including broader use
of high - strength steel that is already in some cars today; better aerodynamics; more efficient air conditioners, transmissions and lighting devices (including headlights); and increased electrification leading to full
hybridization with electric motor and regenerative breaking — all
of which currently exist.
The work
of Marcet - Houben and Gabaldón revealed that,
for yeast,
hybridization was indeed behind the duplication
of some genes.
And the outcomes «may alter the view
of hybridization in animals as some form
of mistake,» comments Loeske Kruuk
of the University
of Edinburgh, «because it is now clear that we can not take the consequences
for fitness at face value.»
For instance, genes that were deleterious only in human - Neanderthal hybrids might have existed, and sexual selection or other forms
of selection against hybrids could have been very important processes during human - Neanderthal
hybridization.
Results
for different genomic partitions, methods, and data types are consistent with or contradict clades in our TENT ExaML, TENT MP - EST *, and exon - only trees and previous studies
of morphology (15), DNA - DNA
hybridization (24), mitochondrial genes (14), and nuclear genes (17).
«The big question
for conservation
of polar bears is if
hybridization occurs rapidly and in combination with other stressors, will that
hybridization have more
of a negative effect now than it did in the past,» says Andrew Whiteley, a geneticist at the University
of Massachusetts, Amherst.
In a molecular analysis including the closely related spinner and striped dolphins, scientists conclude that the clymene dolphin is the product
of natural
hybridization, a process that is more common
for plants, fishes, and birds, but quite rare in mammals.
The major hurdles flagged by WMI are the need
for multiple wild populations,
hybridization with coyotes, the integrity
of the wolf genome, and land ownership patterns in wolf habitat.
«I think we're going to see more
hybridization in the future,» Hanna adds, «so these owls are serving as a case study
for how species that have been isolated
for millions
of years might interact in the face
of a rapidly changing world.»
They plugged the sequences into an algorithm used
for human population modeling, which co-author Daniel Falush modified to allow
for hybridization among strains
of bacteria.
After acetylation by treatment with 0.1 m triethanolamine 0.25 % acetic anhydride, pH 8.0, the tissue was prehybridized (prehybridization solution: 0.75 m NaCl, 25 mm PIPES, 25 mm EDTA, 5 × Denhardt's reagent, 0.2 % SDS, 250 μg / ml Escherichia coli tRNA, and 500 μg / ml salmon testis DNA, pH 6.8) at room temperature
for 5 h. Riboprobes were incubated
for 10 min at 85 °C before application onto the sections and used
for hybridization at a final concentration
of 500 ng / ml.
This allowed the group led by Ole Seehausen (head
of the Fish Ecology and Evolution department at Eawag and Professor
of Aquatic Ecology at Bern University) to provide strong evidence
for his theory that
hybridization between divergent species, in conjunction with ecological opportunity, can facilitate rapid adaptive radiation.
The researchers think these instances
of introgressive
hybridization — a way
for genetic material and, potentially, traits to be passed from one species to another through interspecific mating — are only the first
of many needles waiting to be found in a very large genetic haystack.
Nakhleh said other studies may have missed evidence
of hybridization because the researchers weren't specifically looking
for it.
List
of primers used
for riboprobe generation (in situ
hybridization, ISH) and in RT - PCR experiments
We sampled the bees from the surface
of the bee ball at 0, 30, and 60 min after formation
of the bee ball and examined the Acks - expressing brain regions using in situ
hybridization (n = 5, 7 and 7
for 0, 30 and 60 min, respectively).
As the variance
of admixture has been used to infer the timing
of hybridization and sex - biased admixture, we consider the implications
of assortative mating
for inferring population history and speciation.
Note that panels showing the results
of in situ
hybridization in Figure 4, S3 and S4 are collected from some sections that are used
for the same in situ
hybridization experiments, respectively.
The green line indicates the position
of sections that correspond to a middle part
of the brain used
for this in situ
hybridization experiment.
Statistical analyses
of the number
of Acks signals detected in in situ
hybridization experiments using sections corresponding to the middle brain parts (n = 5, 5, and 4
for 0, 30, and 60 min, respectively) revealed that there was a significant increase in the number
of Acks signals at 30 and 60 min after the bee ball formation in both the Class I and the Class II KCs, and at 60 min after the bee ball formation in the brain area between the DLs and the OLs, and in the OLs (Figure 4S).
The scientists are focusing mainly on annotating both genomes and collecting additional genomic information using «subtractive
hybridization» methods
for related strains
of bacteria.
The development
of high - throughput screening methods such as array - based comparative genome
hybridization (array CGH) allows screening
of the human genome
for copy - number changes.
The team's work highlights this by presenting a comprehensive view
of genome evolution on many different levels (e.g., differences in ploidy, aneuploidy, genetic variants,
hybridization, and introgressions) that is difficult to obtain at the same scale and accuracy
for other eukaryotic organisms.
Oxygen isotope evidence
for large - scale
hybridization of the lower crust during magmatic underplating
The fact that the SNPs used
for each
of these loci fall on the branch uniting divergent haplogroups, which is consistent with ancient vicariance and reciprocal monophyly being attained in the past before
hybridization took place, suggests that high levels
of admixture may have eroded any clinal differentiation in HPRT1.
Geographical location
of the populations sampled
for the transcriptome (red circles) and DNA
hybridization on microarrays datasets (black circles).
An analysis
of potential consequences
of interspecies
hybridization for the aquaculture industry, especially in the context
of changing environmental conditions, was provided.
Possible positive and negative effects
of hybridization were identified, the complexity
of the blue mussel - species complex was highlighted and the implications
of hybridization for adaptation were discussed.
Using our own data and publically available data from array comparative genomic
hybridization (aCGH), we identified a minimal deletion
for the cardiomyopathy associated with del1p36 that included only the terminal 14 exons
of the transcription factor PRDM16 (PR domain containing 16), a gene that had previously been shown to direct brown fat determination and differentiation.
The current secondary contact is thought to result from a range expansion associated with the amelioration
of climatic conditions after the last glacial maximum (∼ 18000 ya)[20], although previous glacial / interglacial cycles during the Pleistocene may have provided multiple opportunities
for vicariant and past
hybridization events.
We obtained polymorphism and divergence data from six individuals
for each
of the rabbit subspecies using Illumina sequencing
of genomic regions enriched by DNA
hybridization on microarrays [60].
We also describe a method
for the bulk testing
of the
hybridization characteristics
of chromosome - specific clones spotted on microarrays by use
of DNA amplified from flow - sorted chromosomes.
In preparation
for in situ
hybridization, tissue specimen slides at room temperature were deparaffinized by soaking in three changes
of Hemo - De solvent (Scientific Safety Solvents, Keller, TX)
for 5 min each, followed by two 1 - minute rinses in 100 % ethanol, an incubation in a solution
of 45 % formic acid (Fisher) / 0.3 % hydrogen peroxide (Calbiochem)
for 15 min, and a rinse in water
for 3 min.
In
hybridization or capture - based target enrichment
of samples prior to next - generation sequencing (NGS), pools
of biotinylated oligonucleotide probes specific
for target regions
of interest are hybridized to a sequencing library in solution.
43) Chari R *, Lockwood WW *, Lam WL (2006) Computational methods
for the analysis
of array comparative genomic
hybridization.
Fluorescence in situ
hybridization (FISH) was used to screen
for the presence
of XMRV genomic sequences in formalin - fixed, paraffin - embedded (FFPE) tissue sections from VP62 (2012) and 19 newly collected tumors (a subset
of the 39 individuals in the prospective study).
Scientists theorize that the reason gluten is causing these digestive system problems is due to the excessive
hybridization of wheat over the last 50 years, which has created newly modified gluten molecules that are foreign to the human digestive system compared to the ancient wheat that humans ate
for several thousand years historically, and even compared to the wheat that your grandparents ate 50 + years ago.
Intestinal permeability was assessed by Ussing chamber; epithelial cell (EC) ultra-structure by electron microscopy; RNA expression
of genes coding
for junctional proteins by Q - real - time PCR; immune response by in - vitro antigen - specific T - cell proliferation and cytokine analysis by cytometric bead array; intestinal microbiota by fluorescence in situ
hybridization and analysis
of systemic antibodies against intestinal microbiota by surface staining
of live bacteria with serum followed by FACS analysis.
Well what happens is, the rapeseed — actually as it was brought over Canada, had gone to a
hybridization form
of growth and process, form
of genetically engineering and it's now
for industrial manufacturing food product purposes — is now being highly heated to allow
for a longer shelf life, but it actually makes it go rancid as a process.
There are lots
of other changes this represents too, such as the presence
of chemicals in our food supply (pesticides, herbicides, xenoestrogens, etc) that didn't exist in the ancient diet, as well as the dramatic changes caused by
hybridization of many
of our foods, which creates versions
of fruits and vegetables bred more
for size, sweetness, and appearance as opposed to natural nutrition... this aspect has decreased micronutrient content in the modern day food supply (just look at wild blueberries vs cultivated blueberries as an example
of that with wild berries coming in at more than DOUBLE the antioxidants from ORAC testing).
When you take this perspective seriously, and also come to understand the level
of hybridization and genetic alterations happening to some
of the most basic foods in this country, like wheat and corn,
for instance, it's no wonder we're sick and it's no wonder our environment is in such trouble.
Farmers have selectively bred wheat species
for specific traits
for hundreds
of years but in the 1960s,
hybridization became a science.
For me, that sort
of fits better with the brand than
hybridization of downsized engines.
These all alloy lightweight high featured engines provide the requisite features
for conventional,
hybridization, (Atkinson cycle mode), and positioned
for the addition
of HCCI partial diesel operating mode.
Commenting on the requirement in the luxury vehicle sector to improve shift speed and refinement as well as fuel consumption, principal engineer Chris Cholmeley notes that a dual clutch transmission is clearly one
of the technical choices
for hybridization.
These test cars will demonstrate the advantages
of bolt - on
hybridization, as well as the range
of benefits that the system delivers
for performance and efficiency.