However, the fact that we detected only 13 — 14 base pair substitutions between the closest mainland gray fox lineage and the northern / southern island fox ancestor suggests that the date of divergence for the island
fox clade could be more recent than 9200 years ago.
Our divergence date estimates for the island
fox clade itself, ~ 9200 years ago, as Santarosae separated into four distinct islands and for the southern island fox lineage, ~ 7100 years ago, correspond well with the earliest AMS dated island fox bones (~ 7100 cal BP for northern and ~ 5640 cal BP for the southern islands).
Not exact matches
Our phylogenetic analysis (Fig. 3) revealed that
fox haplotypes fell in two well - supported and divergent
clades (Clades A
clades (
Clades A
Clades A & B).
Glacial climatic fluctuations caused habitat changes, including the appearance of continental ice sheets as far south as Washington State [37], that may have caused range shifts in locally adapted gray
fox populations, with
foxes with
clade B haplotypes existing as far south as southern California.
As the climate warmed during the Holocene, and suitable habitat expanded northward, gray
fox population ranges shifted further north resulting in
foxes with
clade A haplotypes distributed as far north as Shasta County in northern California (Fig. 1).
Remarkably, island
fox haplotypes formed a monophyletic
clade nested within
clade B, rather than with
foxes from southern California, closest to the Channel Islands.