Interestingly, an increased
fraction of the cell population was found to express CD44 + / CD24 - / ESA + cell surface markers.
Not exact matches
In contrast to the conversion
of alpha
cells, which only concerns a small
fraction of the alpha
cell population, the new mechanism involving delta
cell fate change is a more efficient way
of offsetting the loss
of beta
cells and thus diabetes recovery.
Moreover, all
of these studies compare the pluripotent potential
of the marked ICM - like
population to mixed
fractions that are considered a single further differentiated intermediate
cell type.
While the plating efficiency
of the V+S +
fraction was reduced and produced 4-fold less colonies than the V − S +
fraction, both
fractions gave rise to identical colonies that contain equivalent
populations of V + and V −
cells (Figure 7A, Table 1).
The MID and LOW
populations gave rise to some
of these stem
cell colonies, but formed more mixed or abortive colonies that were partially positive for GCTM - 2 than did the HIGH or NEG
fractions.
Our work indicates that only a small
fraction of the
population resides at the top
of the hierarchy, that lineage priming (co-expression
of stem
cell and lineage specific genes) characterizes pluripotent stem
cell populations, and that extrinsic signaling pathways are upstream
of transcription factor networks that control pluripotency.