The LTQ - Orbitrap Velos MS / MS data were first processed by in - house software DeconMSn [21] accurately determining the monoisotopic mass and charge state of parent ions, followed by SEQUEST search against the IPI database in the same fashion as described above, with the exception that a 0.1 - Dalton mass tolerance for precursor ions and 1 - Dalton mass tolerance for
fragment ions were used.
Not exact matches
They believe these can
be identified by observing the way incoming
ions fragment liquid droplets into multiple smaller droplets.
Mass tolerances of 3 Daltons for precursor
ions and 1 Dalton for
fragment ions without an enzyme defined, as well as static carboxyamidomethylation of cysteine and dynamic oxidation of methionine
were used for the database search.
The
ion at
m / z 153 corresponded to the LPA - specific
fragment ion.
After electrospray ionization, selected
ion fragments were detected and quantified in the API4000 triple - quadrupole tandem MSMS (Applied Biosystems / Sciex).