Sentences with phrase «fragment length»

By the 1990s, DNA testing had advanced somewhat through the use of restriction fragment length polymorphism, but that technique required more material than was available.

The plasmids targeting each gene were respectively transfected into goat fibroblasts, and their genome modification efficiency was determined at day 3 using restriction fragment length polymorphism (RFLP) assay (see Methods).

The DNA has fragmented in an uncontrolled fashion, creating a mix of fragment sizes — genome assemblers must use fragment lengths of specific size ranges.

I'll never forget the euphoric experience when I first got amplified fragment length polymorphism to work properly for my cloning project, or when I first viewed the expression pattern of a newly cloned gene, Hex.

The DNA has fragmented in an uncontrolled fashion, creating a mix of fragment sizes — genome assemblers must use fragment lengths of specific size ranges.

This periodicity in fragment lengths is closely related to the periodicity of the DNA double helix in chromatin, but the two values need not be exactly the same.

Long - read sequencing technologies such as Pacific Biosciences and Oxford Nanopore MinION are capable of producing long sequencing reads with average fragment lengths of over 10,000 base - pairs and maximum lengths reaching 100,000 base - pairs.

Martin and Palumbi did this, using a technique known as restriction fragment length polymorphism analysis.

Gale and his colleagues were the first to develop restriction fragment length polymorphisms for analysing the genomes of cereal crops, and have built on that expertise to construct detailed genetic maps of barley, rye and pearl millet, as well as wheat.

They rotate among several different areas for 1 month each: microanalysis, PCR (polymerase chain reaction), and RFLP (restriction fragment length polymorphism).

The analysis also revealed that the populations of generated tRNA fragments and attributes such as fragment abundance, fragment length, and the fragments» starting and ending points depend on a person's race, population, and gender.

Abbreviations: CFU, colony forming unit; CGH, comparative genome hybridization; CHEF, contour - clamped homogenous electric field; Chr, Chromosome; DSBs, double strand breaks; FACS, fluorescence - activating cell sorting; FLC, fluconazole; NGS, next generation sequencing; NS, niger seed; PFGE, pulsed field gel electrophoresis; RFLP, restriction fragment length polymorphism; SNP, single - nucleotide polymorphism; TS, temperature sensitive

In this study, we used terminal - restriction fragment length polymorphism and 16S rRNA gene sequencing approaches to characterize the bacterial composition of the colonic microflora in a patient suffering from recurrent CDAD before and after treatment by fecal transplantation from a healthy donor.

Site - specific mutations will make the target locus resistant to the restriction enzyme treatment (uncut), which can be detected by restriction fragment length polymorphism (RFLP) assay.

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