Sentences with phrase «free culture medium»

Not exact matches

StemFit Basic02 is a xeno - free, defined medium for human pluripotent stem cell (hiPSC) culture that offers an effective solution for regenerative medicine research.

Expansion of CD34 + cells normalized relative to the values obtained in SFEM medium (dark gray bar) after culturing purified CD34 + cord blood cells for 7 days in StemSpan ™ serum -[SFEM, SFEM II (gold bar)-RSB- and animal component - free ACF (orange bar) media, and six media from other suppliers (light gray bars).

StemSpan ™ serum - free medium undergoes rigorous QC testing to ensure consistent conditions for the culture of HSPCs.

The culture and manipulation of GEMM - ESC clones is performed entirely under feeder - and serum - free conditions using the defined N2B27 medium with LIF and the two inhibitors (2i), CHIR99021 and PD0325901, as originally described by the group of Austin Smith, Cambridge, UK.

Before staining, the medium was replaced with serum - free medium and cultured overnight.

The skin biopsies were washed in Ca / Mg - free Dulbecco's Phosphate Buffered Saline (PBS, Invitrogen, Carlsbad, CA, http://www.invitrogen.com) and minced into approximately 12 smaller pieces before being seeded onto gelatin - coated 6 - well cell culture plates (Corning Enterprises, Corning, NY, http://www.corning.com) containing mouse embryonic fibroblast (MEF) media consisting of Dulbecco's Modified Eagle Medium (DMEM) supplemented with 10 % fetal bovine serum (FBS, Invitrogen) and 100 IU / ml penicillin - streptomycin (Invitrogen), and cultured at 37 °C in a 5 % CO2 incubator.

A cutting - edge initiative that promotes access to culture via digital medium and offers free digital content to 31 million Peruvians.

Informed by the international history of video art, the program traces the development of the medium in Israel and explores how artists have employed technology and material to examine the sociopolitical status quo, through themes such as the prominence of political conflict in mass media; the liberalization of the economy; and the impact of free market politics on Israeli culture.

Extending across the entirety of the museum, the exhibition allows for free association between artists and the themes they address: at once playful and dynamic, works from Ryan Gander, Institute for New Feeling, Liu Wa, and Yangzi invite audiences to explore a wealth of possibilities through combinations of meditation and wry humor; classical mediums of sculpture and painting are reinvented by Yngve Holen and Austin Lee; insidious implications of our hi - tech society are skewered by Lawrence Abu Hamdan and aaajiao; the powers of synthetic materials over human desire are brought to the fore by Sean Raspet and Pamela Rosenkranz; and products of Internet culture are given to refined study with Gillian Wearing and Amalia Ulman.