The field segregates into three major strategies: detecting
free nucleic acids, isolating intact circulating tumor cells, and identifying extracellular vesicles from tumors.
«It's difficult to make contrived samples behave like actual cell -
free nucleic acids, so that's been quite a challenge,» says Kelli Bramlett, director of R&D in clinical sequencing and oncology at Thermo Fisher Scientific in Waltham, Massachusetts.
Not exact matches
To start, she used a cotton swab to get a sample of cells from her cheek, boiled them in a test tube in her kitchen to
free the DNA, then added primers,
nucleic acids that mark the part of the sequence.
These improvements push the boundaries of
nucleic - acid detection and fragment sizing, affecting applications such as single - cell
nucleic acid analysis, PCR -
free preparation of NGS library preparation, bacterial artificial chromosome clone sizing, and exosome analysis.
Concise review: non-cell autonomous reprogramming: a
nucleic acid -
free approach to induction of pluripotency.
Total RNA was isolated using the RNeasy Mini Kit from Qiagen and treated with RNase -
free DNase I (5 units / 100 µg of
nucleic acids, Sigma).
Chlorella's
nucleic content of DNA / RNA have been proven to repair damaged genetic material and restore cells to a healthy and toxin
free state.
When food is browned or caramelized as part of the Maillard reaction, reducing sugars spontaneously react with lipids,
nucleic acids, and aminopeptides, creating advanced glycation end products (AGEs) in a process that generates
free radicals, inflammation, and ensuing intestinal permeability (Vlassara & Uribarri, 2004; Bengmark, 2007).
Vitamin C protects the body's lipids (fats), proteins,
nucleic acids (RNA and DNA), and carbohydrates from damage by
free radicals.