Sentences with phrase «fresh culture medium»
This primary culture was passaged through brief exposure to 0.05 % trypsin - EDTA (Invitrogen) and seeded onto gelatin coated 175 - cm flasks with fresh culture medium.
http://journals.plos.org/ Not exact matches
* 3/4 cup crème fraîche (I used my homemade version) * 3 - 4 tablespoons raw apple cider vinegar * 2 tablespoons cultured buttermilk * 1 handful of fresh basil leaves * 1 handful of fresh cilantro leaves * 1 handful of fresh parsley leaves * 2 medium cloves of garlic, sliced in half, plus more to taste (if you love garlic) * pinch of ground cayenne pepper — optional * sea salt and freshly ground pepper to taste
One - half of the culture medium was replaced with a fresh medium every 3 — 4 days for the 16 DIV.
Each of these tissues was also processed fresh for culture in standard (BSK - H) medium.
After three weeks, all medium from the six - well cultures was removed, followed by a single media wash and replacement with fresh medium for 24 hours.
After 24 hours (on day 1) the mixed viral supernatant was removed, the cells were washed twice with PBS and then cultured in fresh MEF medium.
On day 3 the mixed viral supernatant was again removed, the cells were washed twice with PBS and then cultured in fresh MEF medium.