(E and F) Using ELISA, IL - 2 and IFN - γ production were measured
from culture supernatants of (D) after 72 h of stimulation.
Mcc was purified
from the culture supernatants of E. coli VCS257 cells harboring pJEM15 plasmid, as described earlier (Bieler et al., 2005).
Cell debris was removed
from culture supernatant by centrifugation at 12,500 g for 45 min at 4 °C, and the virus particles were concentrated by ultracentrifugation at 45,000 r.p.m in a BECKMAN 70Ti rotor for 3 hrs.
Not exact matches
Target detectability data
from 858 samples
from 11 independent customer sites, utilizing five unique sample types: serum, plasma, cell
culture supernatant, lysates, and bronchoalveolar lavage.
Vaccinia virus (Western Reserve) recombinant for the codon - optimised gp120 gene
from HIV - 1 (Bx08) expressing gp120 protein in (VERO) cell
culture supernatant.
To characterize the isolate that replicated in HeLa cells, a 166 - bp RNA sequence containing the variable region C of the envelope (Env) surface protein was PCR - amplified
from infected HeLa cell tissue
culture supernatants.
In addition, we showed that, although the
supernatants from each of the HCMV - infected
cultures contained infectious virus, the viral copy number and the quantity and timing of virus production varied among the various organ - specific MSC.