The Hill coefficient was ∼ 1.0 for all culture conditions and did not vary with acute or chronic insulin stimulation in either control cultures or cultures established
from diabetic subjects (P > 0.05, data not shown).
The question is: Do cell cultures, established
from diabetic subjects, conserve the diabetic phenotype in culture?
B: Cell cultures established
from diabetic subjects.
Previous studies of cell cultures established
from diabetic subjects and cultured under basal physiological conditions showed that the basal as well as the insulin - stimulated FV values are reduced (4,5).
Previous studies of cell cultures
from diabetic subjects cultured under basal physiological conditions showed that the basal as well as the insulin - stimulated FV0.1 values are reduced (4,5).
Not exact matches
The aim of the present study was to evaluate glucose transport and GS activity in human satellite cell cultures established
from type 2
diabetic and control
subjects.
ΔA0.5 is lower for the
diabetic group than the control group, but this difference disappears because ΔA0.5 in cultures established
from type 2
diabetic subjects significantly increased with increasing insulin concentration.
GS activity in cultures established
from diabetic and control
subjects at day 8 are shown in Fig. 2.
Furthermore, it is unclear whether the glucose uptake is primarily affected in cultures established
from type 2
diabetic subjects.
J: Significantly enriched KEGG pathways (FDR - adjusted P values < 0.05) of genes that exhibit differential methylation in adipose tissue
from 28
diabetic vs. 28 nondiabetic unrelated
subjects.
Glycogen content was significantly decreased in cultures established
from diabetic patients compared with those established
from control
subjects (Fig. 6C).
K: Differential DNA methylation of IRS1, PPARG, KCNQ1, and TCF7L2 in adipose tissue
from 28
diabetic vs. 28 nondiabetic unrelated
subjects.
We determined the glycogen synthase (GS) activity; the content of glucose -6-phosphate, glucose, and glycogen; and the glucose transport in satellite cell cultures established
from diabetic and control
subjects.
As a new finding, we describe here that impaired GS activity in cell cultures established
from type 2
diabetic subjects can be compensated for by increasing insulin concentrations in a dose - dependent manner.
Distributions of individual sites that exhibit differential DNA methylation in adipose tissue
from 28
diabetic compared with 28 nondiabetic unrelated
subjects in relation to nearest gene regions (H) and CpG island regions (I).
Myotubes established
from type 2
diabetic subjects express a reduced GS mRNA and protein compared with control
subjects (5).
To get further insight into the differences between
diabetic and control cultures, we compared the absolute differences between the basal and the insulin - stimulated state (ΔFV0.1) in control cultures and in those established
from type 2
diabetic subjects (Fig. 3C).
To overcome this limitation, a team led by JAX Assistant Professor Michael Stitzel, Ph.D., isolated single cells
from the pancreatic islets of both non-
diabetic and type 2
diabetic subjects to obtain transcriptomes for each cell.
I even pointed out that one of the studies you posted, after 74 weeks, the reduced calorie, vegan diet
subjects glycated hemoglobin (HbA1C) levels — a critical marker for
diabetics to control — fell by a mere.40 going
from 8.05 (dangerous) to 7.65 (dangerous).
Subjects with renal insufficiency, even subclinical, kidney transplant patients and people with metabolic syndrome or other obesity - related conditions, will be more susceptible to the hypertensive effect of amino acids, especially of the sulphated variety.104 The well - documented correlation between obesity and reduced nephron quantity on raised blood pressure puts subjects with T2D or metabolic syndrome at risk, even if in diabetics with kidney damage the effects are not always consistent with the hypothesis.12, 105,106 In fact, although some authors have reported a positive influence of a reduction in protein intake from 1.2 to 0.9 g / kg, over the short term, on albuminuria in T2D, 107 the same authors have subsequently stated instead that dietary protein restriction is neither necessary nor useful over the long
Subjects with renal insufficiency, even subclinical, kidney transplant patients and people with metabolic syndrome or other obesity - related conditions, will be more susceptible to the hypertensive effect of amino acids, especially of the sulphated variety.104 The well - documented correlation between obesity and reduced nephron quantity on raised blood pressure puts
subjects with T2D or metabolic syndrome at risk, even if in diabetics with kidney damage the effects are not always consistent with the hypothesis.12, 105,106 In fact, although some authors have reported a positive influence of a reduction in protein intake from 1.2 to 0.9 g / kg, over the short term, on albuminuria in T2D, 107 the same authors have subsequently stated instead that dietary protein restriction is neither necessary nor useful over the long
subjects with T2D or metabolic syndrome at risk, even if in
diabetics with kidney damage the effects are not always consistent with the hypothesis.12, 105,106 In fact, although some authors have reported a positive influence of a reduction in protein intake
from 1.2 to 0.9 g / kg, over the short term, on albuminuria in T2D, 107 the same authors have subsequently stated instead that dietary protein restriction is neither necessary nor useful over the long term.108