Not exact matches
The novel thyroid and parathyroid contrast agents differ significantly
from most current NIR contrast agents, which are composed of two separate molecules linked together: a molecule that emits a fluorescent signal, called the
fluorophore, and an antibody or small molecule that targets the tissue of interest.
Ligand - mediated receptor assembly was measured by photon transfer
from the photon donor to the
fluorophore resulting in fluorescence emission.
The result is illumination
from the microlens disk through the pinhole disk for strong excitation of
fluorophores, causing a fluorescence emission which in turn passes through the pinhole disk with high confocality.
TIRF is also very gentle on the sample as the majority of the laser light is reflected away
from the sample and excitation occurs through the
fluorophore's interaction with an evanescent energy field.
CEP8 - SA probe complementary to human chromosome 8 - specific centromeric repetitive sequences and directly labeled with SpectrumAqua
fluorophore was obtained
from Abbott Molecular, Inc. (Des Plaines, IL).
In their JCB 2003 paper, Sekar and Periasamy defined FRET as «a distance - dependent physical process by which energy is transferred non-radiatively
from an excited molecular
fluorophore (the donor) to another
fluorophore (the acceptor) by means of intermolecular long - range dipole - dipole coupling.»