Sentences with phrase «from primary culture»

Fluorescence histograms obtained from primary culture of colon cells (A) and at the time of second sorting cycle (B).

To overcome these limitations, we used two - color flow cytometry to identify and select microvascular endothelial cells from primary cultures obtained from different organs of mice whose tissues harbor a temperature - sensitive SV40 large T antigen (H - 2Kb - tsA58 mice; ImmortoMice).

Not exact matches

Hedstrom's primary focus is print culture, a subject that especially in its middlebrow forms has suffered from scholarly neglect.

From this point of view history can not be understood as a purely immanent development, for it is partially a product of an encounter with a primary reality which transcends culture and gives rise to it.

This produces a fast and irreversible transformation of all cultures from within, globalising the western cultural revolution and its primary consequence, secularisation.

The primary reason apostles were so important in the early church is because they did not have an accurate account of the life of Jesus which they were to follow, or reliable examples of what other local churches were doing, or precise guidance from teachers on how to live and apply the life of Jesus to their lives in their cultures.

More importantly, it is the primary conveyor of a consumer culture whose values system is radically dissonant from biblical faith.

One was the work of a sociologist, Earl Brewer, who, with the aid of a theologian and a ministries specialist, sought by an extensive content analysis of sermons and other addresses given in a rural and an urban church to differentiate the patterns of belief and value constituting those two parishes.67 The second was the inquiry of a religious educator, C. Ellis Nelson, who departed from a curricular definition of education to envision the congregation as a «primary society» whose integral culture conditions its young and old members.68 James Dittes, the third author, described more fully the nature of the culture encountered in the local church.

So men who wish to honor God with their lives and humbly submit to His will should make physical labor their primary occupation, and resist the urge to give in to our culture's glorification of «white collar» work, which is a departure from biblical principles of masculinity.

According to a number of recent studies [1,2,5,13,18], while the culture of sport (including influences from professional and other athletes), as well as the media and other outside sources play a role in the decision of student - athletes to report experiencing concussion symptoms, it is coaches and teammates, along with parents, who have the strongest influence on the decision to report a concussion during sport participation, with coaches being one of the primary barriers to increased self - reporting by athletes of concussive symptoms.

But first, a reminder: VOTE!!!!! Here's a sprawling article about the 33rd Senate District primary race — Gustavo Rivera vs. Pedro Espada Jr. — from a new news and culture site, capitalnewyork.com, that looks like it was created by ex-Observer staffers.

He is also developing a robust and comprehensive panel of 3 - D cell culture models from patient - derived primary cells that can be used to characterize different disease phenotypes and investigate the chemo - response of cells to novel or known drugs.

The research team included Scott H. Randell, Ph.D., a leading expert in the culture of primary lung cells, from the Cystic Fibrosis / Pulmonary Research and Treatment Center at the University of North Carolina at Chapel Hill.

When primary cultures from these mice are stimulated with proinflammatory cytokines, the endothelial cell fraction responds by up - regulating the inducible endothelial cell adhesion molecules, E-selectin and VCAM - 1.

One of the primary objectives of this study was to establish a protocol to facilitate the identification and selection of microvascular endothelial cells from primary organ cultures with a high degree of precision.

Five days post-stimulation the anti-CD3 / anti-CD28 coated magnetic beads were removed from each of the three cultures (non-transduced (NTD), AdX4 - ZFNs, and AdR5 - ZFNs) and 2.5 million cells were seeded in each of four cultures that were subsequently infected with either Bk132 (primary X4 isolate), HxB2 (lab - adapted X4 isolate), R3A (R5X4 primary isolate), or media only (mock).

To further validate our result in clinical samples, we obtained primary tumor from patients with advanced breast cancer and the tissue was passaged once in nonobese diabetic / severe combined immunodeficient mouse without in vitro culture.

C, primary breast cancer cells isolated from patients with advanced cancer were directly infected with KLF4 or control lentivirus, and the CSC population (CD24 − CD44 + ESA +) was measured by FACS after culturing the cells for 72 hours in low - attachment plates.

In 2004 I made the first attempts to grow cultures from primary human tumours.

Primary cultures of cerebellar neurons from neonatal rats allow for examinations of the developmental neurotoxicity of chemicals because the various stages of neurodevelopment — including proliferation, migration, differentiation, and morphological and functional maturation — can be observed in vitro.

In order to modify or expand primary human hematopoietic cells, they must first be isolated from the body and then cultured under conditions that allow for their modification.

The U-formed shape of the dose - response curve for type 2 diabetic cultures and the parallel curves for high insulin concentration in control cultures indicate that the mechanism for the primary defect is different from the mechanism responsible for the induced reduction in acute insulin stimulation of GS.

Named in many ancient cultures, the primary star is commonly called «Muphrid or Murfid» in modern star catalogues, which may have been convolutedly derived from an Arabic reference to the «lance bearer» and associated with nearby Arcturus (Richard Hinckley Allen, 1889: page 104).

Named in many ancient cultures, the primary star is now commonly called «Zavijava» in modern star catalogues, originally from «Al Zawiah,» the Angle or Corner (i.e. Kennel) of the Arab Dogs although Gamma Virginis (commonly called Porrima from the Latin) now fits the location better (Richard Hinckley Allen, 1889: page 469).

U-M's internationally renowned Translational Neuro - Oncology Laboratory generates novel primary cell cultures from pediatric and adult glioma tissue samples to study mechanisms of glioma growth and targeted treatment response.

Primary kidney epithelial cells isolated from human proximal tubule are cultured on the upper surface of an extracellular matrix - coated, porous, polyester membrane that splits the main channel of the device into two adjacent channels, thereby creating an apical «luminal» channel and a basal «interstitial» space.

The primary ingredient in this serum is Malus domestic fruit cell culture cultivated from rare Swiss apple associated with preserving youthful properties of the skin.

Matcha may be used in primary but in our experience does not produce good offspring, so use a culture from your SCOBY Hotel.

Hi: I've just been diagnosed with Candida through a tongue culture from my primary doctor.

We do recommend that you try alternate teas in small batches (such as a one - gallon jar) apart from your main jar, so as to not risk losing your primary culture.

Kate Copping - Westgarth Primary School, Victoria Using Data to Develop Collaborative Practice and Improve Student Learning Outcomes Dr Bronte Nicholls and Jason Loke, Australian Science and Mathematics School, South Australia Using New Technology for Classroom Assessment: An iPad app to measure learning in dance education Sue Mullane - Sunshine Special Developmental School, Victoria Dr Kim Dunphy - Making Dance Matter, Victoria Effective Differentiation: Changing outcomes in a multi-campus school Yvonne Reilly and Jodie Parsons - Sunshine College, Victoria Improving Numeracy Outcomes: Findings from an intervention program Michaela Epstein - Chaffey Secondary College, Victoria Workshop: Developing Rubrics and Guttman Charts to Target All Students» Zones of Proximal Development Holly Bishop - Westgarth Primary School, Victoria Bree Bishop - Carwatha College P - 12, Victoria Raising the Bar: School Improvement in action Beth Gilligan, Selina Kinne, Andrew Pritchard, Kate Longey and Fred O'Leary - Dominic College, Tasmania Teacher Feedback: Creating a positive culture for reform Peta Ranieri - John Wollaston Anglican Community School, Western Australia

One of the things they [architects] also do, which the accountants do too but they create an all - through schools — because we focus on secondary schools (from the age of 11 up to 16)-- but one of the things that the architects would do is that they would combine with a primary school so that they are teaching the children for longer and they are starting to embed the right culture and the right practice and the right behaviour much earlier on.

These materials, produced with input from the Institute of Education Confucius Institute for Schools, will help introduce primary aged children to some of the differences and similarities between the lives, languages and cultures of the people of China and the UK.

The educational force of the wider culture is now the primary site where education takes place, what I have called public pedagogy — modes of education largely produced, mediated, and circulated through a range of educational spheres extending from the new media and old broadcast media to films, newspapers, television programs, cable TV, cell phones, the Internet, and other commercial sites.

For many children, the transition from the last period of early childhood education to the start of primary school is their first experience of a big culture change — in the people surrounding them, the ways in which they interact, their number of peers, the types of activities they are engaged in, and their physical surroundings.

This new resource brings educators primary sources and first - person accounts about a painful period in Canadian history, when about 150,000 Indigenous children were forcibly taken from their families and stripped of their language, culture, and traditions.

We will be joined by Chris Nielson from New Zealand who will be looking at collaboration between primary, secondary and early years settings, Marie - Claire Bretherton from England who will be sharing her experience of peer review as a powerful vehicle for school improvement, leadership development and culture change, Matt Carver from Australia who will share his experience of collaboration in rural and remote communities and Rodney Eckhert and Nancy Sabo in Ontario who will share their collaborative work with elementary school teachers and students.

The Education Endowment Foundation (EEF) and the Royal Society of Arts (RSA) announced five trials to test the impact of different «cultural learning strategies» as part of the «Learning about culture» programme, which is will be rolled out to 9,000 pupils across 400 primary schools from September next year.

A positive culture result obtained from a tracheobronchial wash does not necessarily imply the presence of a clinically significant airway infection and should not lead to antibiotic therapy, unless there was a pure bacterial culture on a primary culture plate.

Culturing the exudate from the pustules on the chin may reveal the presence of yeast and / or bacteria but, again, it isn't clear whether this is a primary or a contributing cause.

While all three artists look to literature as a primary source of inspiration, Bas's fascination with the paranormal and bizarre traces back to his childhood; Chu draws from a wide range of cultures and eras, referencing ancient mythological and ritualistic tales; and Kristalova mines the lost territory of childhood dreams, memories and nightmares.

He is the director of Modern Culture, a visual arts enterprise, specializing in the exhibition and sale of works by a selection of contemporary artists on the primary market and the sale of works, produced from 1956 to the present, on the secondary market.

Comprised of sixteen artworks Seeing through Light: Selections from the Guggenheim Abu Dhabi Collection examines light as a primary aesthetic principle in art, and the theme's flexibility allows for inclusion across cultures and time periods.

CIFO fosters cultural understanding and educational exchange through three primary initiatives: a Grants and Commissions Program for emerging and mid-career visual artists from Latin America; an exhibitions program showcasing work by Latin American artists and international contemporary art from the Ella Fontanals - Cisneros Collection at the CIFO Art Space; and foundation - initiated support for other arts and culture projects.

The American model of development remains one of the primary models for many developed and developing industrial cultures from early 20th Century onward that, of course, require a large supply of fossil energy with current infrastructure.

Two primary objectives of the residential school system were to remove and isolate children from the influence of their homes, families, traditions and cultures, and to assimilate them into the dominant culture.

The primary goal of SEL201 is to help you to understand and implement the pragmatic elements of a successful and sustainable school - wide SEL / SECD and school culture and climate initiative from an organizational / systems perspective.