Sentences with phrase «from tissue culture cells»

The probe we used in the in situ hybridizations was ribosomal RNA labeled with tritiated uridine, and we used Xenopus rRNA because it was available from tissue culture cells.

Not exact matches

Animal cells are cultured from stem cells and incubated in a «bioreactor» into tissue that can be «harvested» and formed into familiar foods like meatballs, patties, and fish sticks.

The team took small pieces of ear tissue from XXY and XYY mice, cultured them, and collected connective tissue cells known as fibroblasts.

Permafrost had preserved tissue from one species — a narrow - leafed campion plant — exceptionally well, so researchers at the Russian Academy of Sciences recently decided to culture the cells to see if they would grow.

Some of the leading non-animal tests are conducted on cell cultures, human and animal corneas from eye banks, corneal tissue cultures, and frozen corneas supplied by hospitals.

The cells used to build the organs could be cultured from stem cells taken from fat or bone - marrow tissue, he says: «We could engineer a blood vessel from your own cells.»

In rats and tissue cultures of human nerve cells, these «beta sheet breakers» not only prevent amyloid plaques from forming, but also dissolve existing plaques.

Until recently, Ain was renowned for a highly prized repository of 18 immortal cancer cell lines, which he developed by harvesting tissue from his patients» tumors after removal, carefully culturing them to everlasting life in vials.

To test their hypothesis, the researchers harvested dermal papillae from seven human donors and cloned the cells in tissue culture; no additional growth factors were added to the cultures.

While there, he worked with cell cultures derived from human fetal tissue.

One postdoc presents data on her efforts to develop an organoid model for small - cell lung cancer; another reports progress on culturing hormone - secreting organoids from human gut tissue.

(Although Clevers's organoids originate from adult stem cells, isolating those cells isn't necessary; culturing a tissue fragment with the right nutrients is enough.)

The substance may be used for biomedical applications, ranging from cell culture and drug delivery to repairing and replacing tissue, organs and cartilage.

The researchers report they were able to transform about one in 5,000 cells — enough to get several iPS cells from a single culture dish — and then coax them to become nerve cells or heart tissue on the benchtop.

Prior research with cultured tissue had shown that a mix of chemicals could change bone marrow stem cells from mice to those resembling brain cells, but when a team led by neurologist Lorraine Iacovitti of Thomas Jefferson University in Philadelphia tried the same brew on human cells, the number altered was modest.

«Our group pioneered the development of cell culture technology for harvesting large numbers of stem cells from human bone marrow and human umbilical cord blood,» Dr. Yeh said, noting that stem cells from these two sources are abundant and can be guided into different types of cells using tissue engineering.

Grima also observed this same clumping of Huntingtin protein with RanGAP1 and nuclear pore proteins to the wrong place in the cell in brain tissue and cultured brain cells derived from deceased patients with Huntington's disease.

Moreover, if embryonic stem cells were cloned from the patient, he or she wouldn't have problems with immunological rejection of the cultured tissue.

Researchers used cultured stem cells derived from their feline patients in the hope of reducing inflammation and promoting tissue regeneration.

To test the quality of the «blood vessels» produced, the researchers cultured a chunk of tissue made from rat liver cells using their technique.

To overcome these limitations, we used two - color flow cytometry to identify and select microvascular endothelial cells from primary cultures obtained from different organs of mice whose tissues harbor a temperature - sensitive SV40 large T antigen (H - 2Kb - tsA58 mice; ImmortoMice).

One of the major impediments to obtaining a large number of endothelial cells from different tissues has been the inability to purify and propagate these cells in culture.

Total RNA was extracted from frozen adipose tissue (100 mg), FACS - isolated cells (> 105), or cultured cells (60 - mm confluent plate) using a commercially available acid - phenol reagent (TRIzol; Invitrogen Corp.).

Specifically, cell cultures from bone tissue were incubated in 10 % DMEM containing 10 ng / ml TNF - α and 10 μg / ml fluorescent probe of acetylated LDL, DiI - Ac - LDL (Biochemical Technologies, Cambridge, MA), for 4 h. Cells were harvested in the manner described above with the exception that during this labeling period, the rat anti-E-selectin mAb (10E9.6) conjugated to FITC replaced PE - conjugated 10E9.6.

We also do culture of cells and tissues isolated from mice to manipulate signaling events in controlled, measurable ways.

We then prepared cultures from this tissue, but we observed only very few GFAP or S100β positive astrocytes, while the majority of cells were negative for all glial and neuron markers tested, suggesting that these cells may be non-neural.

Spleen cells (3 × 107) from either experimental or control effector lymphocytes were dispensed into 75 - cm2 tissue culture flasks along with 20 ml of boosting medium and 6 × 105 γ - irradiated (3,000 rad) BALB / c spleen cells (erythrocytes lysed).

In the current study, lead authors David V. Hansen, PhD, a postdoctoral fellow, and Jan H. Lui, a graduate student in the Kriegstein lab, examined the OSVZ, using new labeling and tracking techniques to follow individual cells and their progeny over time in cultured tissue slices from fetal cortex tissue that had been donated for research.

This work involves behavior, immunohistochemistry (of brain tissue from mouse models of Alzheimer's), cell culture, and in vitro biochemistry.

Nakabayashi, Monk, and collaborators studied methylation patterns in adult and umbilical blood and placenta cells from healthy volunteers; brain tissue from a brain bank; and a cultured liver cell line.

Tissue engineering is bringing together advances from stem cell biology, microfluidics, robotics, and 3 - D cell culture to develop novel products...

After initial testing demonstrated that their senoablative constructs could eliminate up to 80 % of senescent cells in cell culture, the Oisín team tested them out in living mice, showing that it can purge senescent cells from multiple tissues in middle - aged animals.

Bone marrow - derived mesenchymal stem cells (BM - MSCs) are obtained by isolating mononucleated cells from a marrow aspirate by centrifugation on a density gradient, followed by adherent cell expansion in tissue culture.

Based on this finding, the researchers believe there must be a signal from the lymphoid tissue cells that influences the blood T cells when they are cultured together, causing the blood cells to behave like lymphoid tissue cells.

These epithelial organoid cultures are genetically and phenotypically extremely stable, allowing transplantation of the cultured offspring of a single stem cell, as well as disease modeling by growing organoids directly from diseased patient tissues (45).

In a curious twist resembling cellular alchemy, when blood T cells are cultured with lymphoid tissue from the tonsils or spleen, the blood T cells begin acting like lymphoid tissue T cells, becoming more activated and more susceptible to abortive infection and death by pyroptosis.

Researchers had developed the technologies needed to create organoids years before — how to grow cells in culture, how to isolate stem cells from human tissue, and how to coax the stem cells, undifferentiated and immature, to become specific types of cells at later stages of development.

To characterize the isolate that replicated in HeLa cells, a 166 - bp RNA sequence containing the variable region C of the envelope (Env) surface protein was PCR - amplified from infected HeLa cell tissue culture supernatants.

U-M's internationally renowned Translational Neuro - Oncology Laboratory generates novel primary cell cultures from pediatric and adult glioma tissue samples to study mechanisms of glioma growth and targeted treatment response.

Their three - dimensional cell - culture system, adapted for high - throughput screening, has enabled them to identify small molecules that can inhibit adhesion and invasion, preventing ovarian cancers from spreading to nearby tissues.

Such stem cells are «immortal» in the lab, meaning there is no need for new cell cultures or re-harvesting from additional tissue samples.

Cell culture studies involve removing tissue (or even individual cells) from a plant or animal and growing them in a laboratory environment.

Cheng and his colleagues cultured lung spheroid cells from these tiny tissue samples until they were numerous enough — in the tens of millions — to be delivered therapeutically.

Cheng and Lobo have focused on a set of stem cells and support cells that reside in the lungs and can be reliably cultured from biopsied lung tissue.

ACT - activated clotting time (bleeding disorders) ACTH - adrenocorticotropic hormone (adrenal gland function) Ag - antigen test for proteins specific to a disease causing organism or virus Alb - albumin (liver, kidney and intestinal disorders) Alk - Phos, ALP alkaline phosphatase (liver and adrenal disorders) Allergy Testing intradermal or blood antibody test for allergen hypersensitivity ALT - alanine aminotransferase (liver disorder) Amyl - amylase enzyme — non specific (pancreatitis) ANA - antinuclear antibody (systemic lupus erythematosus) Anaplasmosis Anaplasma spp. (tick - borne rickettsial disease) APTT - activated partial thromboplastin time (blood clotting ability) AST - aspartate aminotransferase (muscle and liver disorders) Band band cell — type of white blood cell Baso basophil — type of white blood cell Bile Acids digestive acids produced in the liver and stored in the gall bladder (liver function) Bili bilirubin (bile pigment responsible for jaundice from liver disease or RBC destruction) BP - blood pressure measurement BUN - blood urea nitrogen (kidney and liver function) Bx biopsy C & S aerobic / anaerobic bacterial culture and antibiotic sensitivity test (infection, drug selection) Ca +2 calcium ion — unbound calcium (parathyroid gland function) CBC - complete blood count (all circulating cells) Chol cholesterol (liver, thyroid disorders) CK, CPK creatine [phospho] kinase (muscle disease, heart disease) Cl - chloride ion — unbound chloride (hydration, blood pH) CO2 - carbon dioxide (blood pH) Contrast Radiograph x-ray image using injected radiopaque contrast media Cortisol hormone produced by the adrenal glands (adrenal gland function) Coomb's anti- red blood cell antibody test (immune - mediated hemolytic anemia) Crea creatinine (kidney function) CRT - capillary refill time (blood pressure, tissue perfusion) DTM - dermatophyte test medium (ringworm — dermatophytosis) EEG - electroencephalogram (brain function, epilepsy) Ehrlichia Ehrlichia spp. (tick - borne rickettsial disease) EKG, ECG - electrok [c] ardiogram (electrical heart activity, heart arryhthmia) Eos eosinophil — type of white blood cell Fecal, flotation, direct intestinal parasite exam FeLV Feline Leukemia Virus test FIA Feline Infectious Anemia: aka Feline Hemotrophic Mycoplasma, Haemobartonella felis test FIV Feline Immunodeficiency Virus test Fluorescein Stain fluorescein stain uptake of cornea (corneal ulceration) fT4, fT4ed, freeT4ed thyroxine hormone unbound by protein measured by equilibrium dialysis (thyroid function) GGT gamma - glutamyltranferase (liver disorders) Glob globulin (liver, immune system) Glu blood or urine glucose (diabetes mellitus) Gran granulocytes — subgroup of white blood cells Hb, Hgb hemoglobin — iron rich protein bound to red blood cells that carries oxygen (anemia, red cell mass) HCO3 - bicarbonate ion (blood pH) HCT, PCV, MHCT hematocrit, packed - cell volume, microhematocrit (hemoconcentration, dehydration, anemia) K + potassium ion — unbound potassium (kidney disorders, adrenal gland disorders) Lipa lipase enzyme — non specific (pancreatitis) LYME Borrelia spp. (tick - borne rickettsial disease) Lymph lymphocyte — type of white blood cell MCHC mean corpuscular hemoglobin concentration (anemia, iron deficiency) MCV mean corpuscular volume — average red cell size (anemia, iron deficiency) Mg +2 magnesium ion — unbound magnesium (diabetes, parathyroid function, malnutrition) MHCT, HCT, PCV microhematocrit, hematocrit, packed - cell volume (hemoconcentration, dehydration, anemia) MIC minimum inhibitory concentration — part of the C&S that determines antimicrobial selection Mono monocyte — type of white blood cell MRI magnetic resonance imaging (advanced tissue imaging) Na + sodium ion — unbound sodium (dehydration, adrenal gland disease) nRBC nucleated red blood cell — immature red blood cell (bone marrow damage, lead toxicity) PCV, HCT, MHCT packed - cell volume, hematocrit, microhematocrit (hemoconcentration, dehydration, anemia) PE physical examination pH urine pH (urinary tract infection, urolithiasis) Phos phosphorus (kidney disorders, ketoacidosis, parathyroid function) PLI pancreatic lipase immunoreactivity (pancreatitis) PLT platelet — cells involved in clotting (bleeding disorders) PT prothrombin time (bleeding disorders) PTH parathyroid hormone, parathormone (parathyroid function) Radiograph x-ray image RBC red blood cell count (anemia) REL Rocky Mountain Spotted Fever / Ehrlichia / Lyme combination test Retic reticulocyte — immature red blood cell (regenerative vs. non-regenerative anemia) RMSF Rocky Mountain Spotted Fever SAP serum alkaline phosphatase (liver disorders) Schirmer Tear Test tear production test (keratoconjunctivitis sicca — dry eye,) Seg segmented neutrophil — type of white blood cell USG Urine specific gravity (urine concentration, kidney function) spec cPL specific canine pancreatic lipase (pancreatitis)-- replaces the PLI test spec fPL specific feline pancreatic lipase (pancreatitis)-- replaces the PLI test T4 thyroxine hormone — total (thyroid gland function) TLI trypsin - like immunoreactivity (exocrine pancreatic insufficiency) TP total protein (hydration, liver disorders) TPR temperature / pulse / respirations (physical exam vital signs) Trig triglycerides (fat metabolism, liver disorders) TSH thyroid stimulating hormone (thyroid gland function) UA urinalysis (kidney function, urinary tract infection, diabetes) Urine Cortisol - Crea Ratio urine cortisol - creatine ratio (screening test for adrenal gland disease) Urine Protein - Crea Ratio urine protein - creatinine ratio (kidney disorders) VWF VonWillebrands factor (bleeding disorder) WBC white blood cell count (infection, inflammation, bone marrow suppression)

The procedure is extensive as it requires adipose (fat) tissue to be taken from the animal and submitted for cell culture.

Professional Duties & Responsibilities Biomedical and biotechnology engineer with background in design of biomaterials, biosensors, drug delivery devices, microfrabrication, and tissue engineering Working knowledge of direct cell writing and rapid prototyping Experience fabricating nanocomposite hydrogel scaffolds Proficient in material analysis, mechanical, biochemical, and morphological testing of synthetic and biological materials Extensive experience in bio-imaging processes and procedures Specialized in mammalian, microbial, and viral cell culture Working knowledge of lab techniques and instruments including electrophoresis, chromatography, microscopy, spectroscopy, PCR, Flow cytometery, protein assay, DNA isolation techniques, polymer synthesis and characterization, and synthetic fiber production Developed strong knowledge of FDA, GLP, GMP, GCP, and GDP regulatory requirements Created biocompatible photocurable hydrogels for cell immobilization Formulated cell friendly prepolymer formulation Performed surface modification of nano - particle fillers to enhance their biocompatibility Evaluated cell and biomaterial interaction, cell growth, and proliferation Designed bench - top experiments and protocols to simulate in vivo situations Designed hydrogel based microfluidic prototypes for cell entrapment and cell culture utilizing computer - aided robotic dispenser Determined various mechanical, morphological, and transport properties of photocured hydrogels using Instron, FTIR, EDX, X-ray diffraction, DSC, TGA, and DMA Assessed biocompatibility of hydrogels and physiology of entrapped cells Evaluated intracellular and extracellular reactions of entrapped cells on spatial and temporal scales using optical, confocal, fluorescence, atomic force, and scanning electron microscopies Designed various biochemical assays Developed thermosensitive PET membranes for transdermal drug delivery application using Gamma radiation induced graft co-polymerization of N - isopropyl acylamide and Acrylic acid Characterized grafted co-polymer using various polymer characterization techniques Manipulated lower critical solution temperature of grafted thermosensitive co-polymer Loaded antibiotic on grafted co-polymer and determined drug release profile with temperature Determined biomechanical and biochemical properties of biological gels isolated from marine organisms Analyzed morphological and mechanical properties of metal coated yarns using SEM and Instron Performed analytical work on pharmaceutical formulations using gas and high performance liquid chromatography Performed market research and analysis for medical textile company Developed and implement comprehensive marketing and sales campaign