Sentences with phrase «from wild mice»

The immunological benefits from the wild mice's gut bacteria may, in part, explain a persistent problem in disease research: Why disease experiments in lab mice, such as vaccine studies, turn out very differently in humans or other animals.

This is a visualization of the process of transferring gut microbiota from wild mice to laboratory mice.

The researchers confirmed that C57BL / 6 mice had distinct gut microbiomes from wild mice.

«Gut bacteria from wild mice boost health in lab mice.»

Now, immunologists at the National Institute of Diabetes and Digestive and Kidney Diseases in Bethesda, Maryland, have contaminated lab mice in a different way: by giving them gut bacteria from wild mice.

The gut bacteria from the wild mice were fairly similar, but the microbiomes of the lab mice were significantly different, with fewer Bacteroidetes and Proteobacteria, for example.

Researchers aware of this reality have now shown that transplanting gut bacteria from wild mice into «clean» lab mice has made those rodents less likely to die from the flu or develop cancer.

Not exact matches

The researchers then transplanted the gut bacteria from healthy, wild Maryland mice (which were most similar genetically to the lab mice) into the lab mice.

Barbara Rehermann and Stephan Rosshart first collected 800 wild house mice (Mus musculus domesticus) in the Washington, D.C., metro area, and compared their DNA and gut bacteria both with a lab mouse strain and with wild mice from all over the world.

Hur added, «To investigate whether Cpf1 had off - target effects, we performed whole genome sequencing using genomic DNA isolated from one Foxn1 mutant mouse and its wild - type sibling.

Activated, wild type B cells from female mice tightly localize Xist RNA at their inactive X chromosomes (left), whereas Xist RNA becomes dispersed throughout the entire nucleus when YY1 is deleted (right).

These organoids had more stem cells than those isolated from wild - type mice.

Probing the link between adiponectin deficiency and metabolic disorders like diabetes and obesity, researchers from the Albert Einstein College of Medicine in New York City injected adiponectin into wild - type mice, diabetic mice, and obese diabetic mice.

In neurons of DIXDC1 mutant mice (center) the dendrites — neural antennae that receive input from other brain cells — have fewer of the dendritic spines (white with red arrows)-- the receiving half of most synaptic inputs — compared to dendrites in wild type mice (left).

(Left) Blood smears from anemic mice indicate irregular shapes of red blood cells; (right) wild type mice indicate normal shapes of red blood cells.

The researchers followed up with two experiments: in one, they injected vasopressin into the brains of mouse parents from both of the wild species, and in the other, they genetically manipulated vasopressin neurons in the brains of house mice (Mus musculus) to excite them.

Professor Mark Viney and colleagues from the University of Bristol and the London School of Hygiene & Tropical Medicine studied the immune systems of 460 wild mice taken from 12 sites in the UK and compared them with mice bred in captivity.

He was part of a team that found that, in mice with mitochondria from both lab and distantly related wild populations, one mitochondrial lineage tended to dominate.

No significant differences in bone growth were seen in wild type mice that received rosuvastatin or vehicle (right and second from right).

All clones with the wild type Tyr302, including the Envs from the viral innoculum and two Envs from X4 - ZFN mice utilized CXCR4 approximately 1000-fold more efficiently than CCR5 and comparably to the X4 - tropic control TYBE (Figure 6C).

It suggests that the early hunter - gatherer settlements transformed ecological interactions and food webs, allowing house mice that benefited from human settlements to out - compete wild mice and establish themselves as the dominant population.

Numbers represent percent increases relative to wild type mice and were calculated from the data shown in Table 1.

Moreover, loss of myostatin activity resulting either from postnatal inactivation of the Mstn gene [3], [4] or following administration of various myostatin inhibitors to wild type adult mice [5]--[7] can also lead to significant muscle growth.

Finally, from about 11,500 years ago, when people in the region began farming and became sedentary, the wild mice were displaced again (PNAS, doi.org/b4wn).

The researchers trapped more than 800 wild mice from eight locations across Maryland and the District of Columbia to find healthy, suitable candidates for a gut microbiota donation.

Four generations later, the mice still carried either the wild microbiomes or the control laboratory microbiomes passed down from their foremothers.

In the most extreme case, muscle weights in F66 / Mstn − / − mice were increased by 250 — 350 % from those seen in wild type mice (Figures 2b and 3).

They observed a significant decrease in the number of proliferating stem cells in the brains of HIV / gp120 - mice compared with similar tissue from normal, wild - type mice.

Male or female (5 - 6 wks old) wild type and genetically modified mice (as required for individual investigator's protocols) will be fed a high fat high sucrose diet (from Research Diets ranging in fat from 45 - 58 % of calories as fat, 26 - 39 % carbohydrate (sucrose) and 16 % protein or control diets (10 % fat, 73 % carbohydrate, 16.4 % protein or rodent chow) for up to 16 weeks.

The goal of this protocol (written in French) is to describe the steps and quality controls to prepare new stable murine embryonic stem cells lines: ① either from wild type mouse embryos to introduce a mutation by genetic targeting or homologous recombination to get a genetically modified mouse line ② or from genetically modified mouse embryos with two aims:

The present findings are derived from studies using CD4KO mice and thus, raise the question as to whether the CD4 + T cell - independent immune mechanisms in CD4 KO mice differ from those involved in corneal allograft rejection in wild - type mice whose CD4 + T cells population have been depleted with monoclonal antibodies.

Histopathological examination of rejected corneal allografts in CD4 KO mice revealed a mixed inflammatory infiltrate containing large numbers of neutrophils and mononuclear cells, which was indistinguishable from the infiltrate seen in rejected corneal allografts in wild - type mice (data not shown).

Their content is optimized for genetic mapping in the Collaborative Cross and Diveristy Outbred populations; for discriminating haplotypes from common laboratory strains and substrains; and for evaluating the ancestry of wild - caught mice.

Membrane resistance and rheobase in both dSPN and iSPNs from 2 - month old Q175 mice did not significantly differ from Wild - type (WT) dSPN and iSPNs respectively, suggesting that deficits in SPN excitability emerged largely in parallel in each subtype.

Radiation - induced thymic lymphomas from a wild - type mouse (A) and from a p21 - deficient mouse (B).

Overexpression of Galgt2 in skeletal muscle prevents injury resulting from eccentric contractions in both mdx and wild - type mice.

In striking contrast, wild - type LCMV Armstrong, from which these variants were generated, induces a potent CTL response in immunocompetent mice and the LCMV infection is rapidly cleared.

C57BL / 6 (wild type) and perforin - deficient (C57BL / 6 - Prf 1tm1Sdz / J, Prf1 − / −) mice were obtained from the Jackson Laboratory and bred in the Abramson Research Center Animal Facility at The Children's Hospital of Philadelphia.

Isolated right and left atria and small mesenteric arteries from wild type and tau deficient (KO)... mice of two age groups (13 and 23 months old) were used to assess cardiovascular phenotypes.

Given that our wild type B. burgdorferi acquired by xenodiagnostic ticks retain the ability to express ospC (also shown by RT - PCR in Fig 4), we can not rule out the possibility that a tick midgut - adapted phenotype, in the absence of salivation and feeding, contributed to the failure of the B. burgdorferi acquired from XT to infect SCID mice.

Brain, eyes, and optic nerves were taken from transgenic and wild - type mice of 3 to 12 months of age and processed for immunohistochemistry, qPCR, or western immunoblotting.

(A) Demonstration of Slc6a14 mRNA up - regulation in PyMT - driven mammary tumours in mice; N, mammary tissue from wild - type mice; T, mammary tumour tissue from PyMT - Tg mice.

«What this might suggest is that transfer of some of the «good» microbiota from wild - type mice to replace the «bad» microbiota from mice lacking AIM2 offers increased protection against colorectal cancer,» Man said.

Analysis of transcriptomes in tumour tissues from wild - type mice and Slc6a14 - null mice indicated no compensatory changes in the expression of any other amino acid transporter mRNA.

The next slide actually shows you one of the slides from this publication, showing you that this is the wild - type mice; males on the left and females on the right.

(A) Genotype documentation of - / y hemizygous males and - / - homozygous females; evidence of Slc6a14 deletion in colon and lung tissues from - / y hemizygous males and - / - homozygous females; expression of Slc6a14 in colon (C) and lung (L) but not in mammary gland (M) in wild - type (WT) female mice.

We examined the expression of Slc6a14 in breast tumours obtained from 4 - month - old PyMT - Tg female mice and in mammary glands obtained from age - matched wild - type female mice.

Because the SD of brain progranulin in wild - type mice was about 10 % of the mean level, progranulin levels in Grn + / − mice would need to increase from 50 % of normal to 80 % of normal, i.e. by 30 % of the normal value, to reach the lower limit of normal.

Frozen sections of lung and colon tissues from wild - type mice and Slc6a14 − / − mice were also used for immunofluorescence analysis of Slc6a14 protein expression with an antibody raised against a mouse peptide sequence [17].

(A) Knockout mice are indistinguishable from those of wild type in morphology and size.