Not exact matches
This maneuver «
froze» the
cells in a quiescent phase of their division cycle and may have made their chromosomes more susceptible to being reprogrammed to initiate the growth of a new organism after the nuclei were transferred into an
egg.
As a radical alternative, biologist Jeanne Loring at the Scripps Research Institute is attempting to transform
frozen skin
cells from threatened species into
eggs and sperm.
With more - than -30-year-old
frozen tissues from a preserved frog, the team extracted and implanted the nucleus of a dead
cell into a fresh host
egg from a distantly related species.
«Maybe at the one -
cell or two -
cell stage,» Eggan and his colleagues reasoned, «there's still some of that stuff in there...» And if they picked the right moment of
cell division, when these powerful reprogramming factors were still floating around in the periphery of the
cell, they might be able to use drugs to temporarily
freeze the
cell in the middle of division, stick in the needle of a micromanipulator to suck out the embryonic DNA, squirt in DNA from an adult animal, and then kick - start the process of reprogramming — hours, perhaps even days after an
egg had been fertilized.
Because fertilized human embryos are far more accessible than unfertilized
eggs, which can not be
frozen and stored, extending the result to humans could lower the practical barriers against creating human embryonic stem
cells to study and potentially treat disease.
The procedure went smoothly, and the
cells were eventually added to the
Frozen Zoo, the menagerie of
cells,
eggs, sperms, and embryos of about 1,000 species and subspecies, hosted at the San Diego Zoo.