Sentences with phrase «fusion protein»

We recommend the full - length fusion protein procedure if your protein of interest has low sequence similarity with other proteins.

A mutation in either gene causes it to combine with a partner gene to express an abnormal fusion protein that acts along a cellular growth pathway to result in cancer.

Clade C recombinant p17 / 24 - B protein, expressed as His - tag fusion protein in E.coli using expression vector pET24 and purified using Talon Superflow metal affinity resin.

Our studies and those of others (5, 6, 7, 10, 11) highlight the very real consequences of misfolded dark FP fusions including erroneous quantitation of fusion protein levels.

Clade B recombinant Nef protein, expressed as a His - tag fusion protein in E. coli using expression vector pET24 and purified using Ni - agarose columns.

May be used for the affinity purification of the SIV - Pk fusion protein from bacterial expression vectors 282 and 283.

GST and GST - Sp1 fusion proteins were purified and analyzed by SDS - PAGE and Coomassie blue staining (bottom).

In the experiments described in the paper, the MGH team confirmed that their mesothelin - targeting fusion protein binds to mesothelin on either ovarian cancer or mesothelioma cells, activates dendritic cells, and enhances the cells» processing and presentation of several different tumor antigens, inducing a number of T - cell - based immune responses.

For the current study, Snell and his colleagues at UT Southwestern used sophisticated computer analysis tools to compare the amino acid sequence of Chlamydomonas HAP2 with that of known viral fusion proteins.

We also show that transgene - encoded GFP fusion proteins of human Perilipins 1 and 2 localize in C. elegans similarly as W01A8.1:: GFP on vesicular structures that are positive for lipid content.

Figure 1: FGFR - TACC fusion protein disrupts cellular division (mitosis) by localizing aberrantly at the mid-body of dividing cells.

By analogy to the gag / abl fusion protein of Abelson murine leukemia virus, the replacement of amino terminal c - abl sequences by bcr sequences in P210 may create a transforming protein involved in CML.

To test this possibility, an in vitro GST fusion protein pull - down assay was done.

The newly issued patents (U.S. Patent Nos. 9493515 and 9493516) broadly cover Proclara's core technology, including methods of treating protein misfolding diseases and pharmaceutical compositions comprising the company's lead molecule, NPT088, a GAIM - Ig fusion protein currently being evaluated in a Phase 1b study for the treatment of Alzheimer's disease.

«Design and use of fluorescent fusion proteins in cell biology.

Because GDF15 has a short plasma half - life and is difficult to produce in substantial quantities, the scientists generated two different fusion proteins that were more stable in the circulation and led to higher yields.

To examine this limb of the secretory pathway, we have examined two membrane proteins: a biosynthetic enzyme, chitin synthase III (Chs3p), and a mating cell fusion protein, Fus1p, which travel from the TGN / endosome membrane to the plasma membrane in yeast.

GT's lead oncology drug candidate, OXS - 1550 (DT2219) is a novel bispecific scFv recombinant fusion protein - drug conjugate composed of the variable regions of the heavy and light chains of anti-CD19 and anti-CD22 antibodies and a modified form of diphtheria toxin as its cytotoxic drug payload.

Chinnaiyan and colleagues then conducted studies using cancer cells and found that the different FGFR fusion proteins all seemed to drive cancer cell proliferation by activating FGFR signaling.

To overcome the structural flexibility of the β2AR and to facilitate its crystallization, we engineered a β2AR fusion protein in which T4 lysozyme (T4L) replaces most of the third intracellular loop of the GPCR («β2AR - T4L») and showed that this protein retains near - native pharmacologic properties.

This is a confocal image of a live mammalian cell expressing a GFP - Ebola VP40 fusion protein (green).

Cancer cells are notorious for their genomes gone haywire, often yielding fusion proteins — mash - ups of two disparate genes that, once united, assume new and harmful capabilities.

Although insufficient on its own to trigger leukemia, f - circM9 appears to work together with other cancer - promoting signals (such as the MLL - AF9 fusion protein) to cause disease.

Curious about the possibility of circular RNAs contributing to cancer, Pandolfi and his colleagues set out to see if they could detect relevant changes in tumors known to harbor distinct fusion proteins, which result when different chromosomes abnormally join together, melding two separate genes into a new centaur - like gene.

We report the crystal structure of a human β2 - adrenergic receptor — T4 lysozyme fusion protein bound to the partial inverse agonist carazolol at 2.4 angstrom resolution.

The IM30 fusion protein provides a starting point for future research, unraveling new types of membrane fusion mechanisms in chloroplasts and blue - green algae.

A phase I clinical trial and a phase III clinical trial have been completed for a T - cell based immunotherapy and IL - 13 bacterial toxin fusion protein respectively, both with promising outcomes.

We encourage investigators to characterize FP - fusion proteins functionally relative to the untagged protein of interest.

In our experience, full - length fusion protein antigens usually generate antibodies with higher titers and higher sensitivities.

To generate anti-DDX3 antibody, glutathione S - transferase fusion protein containing the NH2 - terminal 1 - 226 amino acid fragment of DDX3 [GST / DDX3 (1 - 226)-RSB- was used as antigen for immunization.

FP misfolding can even disrupt fusion protein localization.

Their basic approach is to use fluorescence microscopy to follow a GFP - Mig1p fusion protein in a single cell, something that has only become possible recently.

Targeted DNA demethylation and activation of endogenous genes using programmable TALE - TET1 fusion proteins.

Targeted DNA methylation in vivo using an engineered dCas9 - MQ1 fusion protein.

There is hard evidence that no unintended aberrant fusion proteins are formed.

Calibration curves correlating this ratio to the total expression time can therefore be used to determine how long a pool of FP timers has been expressed, either in whole cells or as a pool of fusion proteins localized to a specific region of the cell.

To do so, she generated fusion proteins in which Dronpa domains flanked either side of an enzyme's active site.

Caussinus, E., Kanca, O., and Affolter, M. «Fluorescent fusion protein knockout mediated by anti-GFP nanobody.»

A trpE / apoA - IV fusion protein was generated to produce a goat antibody to human apoA - IV.

Thus far Plasmids 101 has covered GFP and its related fluorescent proteins, which are sometimes used as tags for detection; however, those are just one (admittedly large) class of common fusion protein tags.

The expression vector contained the cDNAs coding for a potential fusion protein containing the GFP (EGFP), the 2A esterase of foot - and - mouth disease virus (FMDV), and HOXB4 with an additional N - terminal hemagglutinin (HA) epitope tag (eGFP2AHOXB4).

We experimentally developed Trg fusions proteins, which bind the ligand - PBP complex, and activate intracellular PhoR, the HK cognate of PhoB.