Sentences with phrase «galactosidase in»

A. Bartesaghi et al. 2.2 Å resolution cryo - EM structure of β - galactosidase in complex with a cell - permeant inhibitor.

The Tetrahymena intervening sequence (IVS) has been inserted into the gene for the alpha - donor fragment of beta - galactosidase in a recombinant plasmid.

Not exact matches

Coupling of Tetrahymena ribosomal RNA splicing to beta - galactosidase expression in Escherichia coli

In mice heterozygous for tau - lacZ targeted to the melanopsin gene locus, β - galactosidase — positive RGC axons projected to the SCN and other brain nuclei involved in circadian photoentrainment or the pupillary light refleIn mice heterozygous for tau - lacZ targeted to the melanopsin gene locus, β - galactosidase — positive RGC axons projected to the SCN and other brain nuclei involved in circadian photoentrainment or the pupillary light reflein circadian photoentrainment or the pupillary light reflex.

In the current test, these were the molecules p16, p21 and a positive test for beta - galactosidase activity.

In one example, Subramaniam's team recently solved the structure of a relatively small protein, β - galactosidase, to a resolution of 2.2 Å.

Brandon Hall et al (2017) p16 (Ink4a) and senescence - associated β - galactosidase can be induced in macrophages as part of a reversible response to physiological stimuli (http://www.aging-us.com/article/101268/text) or (https://doi.org/10.18632/aging.101268)

B. Hall et al (2016) Aging of mice is associated with p16 (Ink4a)- and β - galactosidase - positive macrophage accumulation that can be induced in young mice by senescent cells (http://www.aging-us.com/article/100991)

Our lead candidate, LYS - GM101, has been designed to replace this defective gene in the cells of GM1 patients, in order to allow for production of the functional lysosomal acid beta - galactosidase (bgal) enzyme.

In the picture depicted, one of the two copies of the gene of the mouse was replaced with the lac Z cassette expressing the bacterial beta galactosidase and its activity in the kidney is followed by staininIn the picture depicted, one of the two copies of the gene of the mouse was replaced with the lac Z cassette expressing the bacterial beta galactosidase and its activity in the kidney is followed by staininin the kidney is followed by staining.

In their terminally nonproliferative state both p21 + / + and p21 - / - cultures were positive for the senescence - associated beta - galactosidase (SA - beta - gal) activity; in contrast, the labeling index of p21 + / + cells was low (< 5 %) whereas the labeling index of p21 - / - cells was high (> 30 %In their terminally nonproliferative state both p21 + / + and p21 - / - cultures were positive for the senescence - associated beta - galactosidase (SA - beta - gal) activity; in contrast, the labeling index of p21 + / + cells was low (< 5 %) whereas the labeling index of p21 - / - cells was high (> 30 %in contrast, the labeling index of p21 + / + cells was low (< 5 %) whereas the labeling index of p21 - / - cells was high (> 30 %).

Selection for expression of the gene requires transcription from a cellular promoter, and consequently a mutation in a cellular gene, and the activity of the tagged gene can be followed by staining for beta galactosidase activity.

In vitro, Pds has been shown to produce β - glucosidase, N - acetyl - β - glucosaminidase, acid and alkaline phosphatases, esterase / esterase lipase / lipase, leucine and vailine arylamidase, naphthol - AS - B1 - phophohydrolase, various proteinases (albumin / casein / gelatin), and urease, while no enzymatic activity was indicated for cystine arylamidase, α - chymotrypsin, alpha / beta galactosidase, trypsin, β - glucoronidase, α - fucosidase, and α - mannosidase [11].

In this approach, a promoterless reporter gene (for instance encoding beta galactosidase, or beta geo) is introduced into ES cells.

Importantly, although cells in prostate cancer cell holoclones were homogenously small and senescence - associated β - galactosidase (SA - βgal) negative, most cells in paraclones were flat and large and SA - βgal positive (Supplementary Fig.

Alpha - galactosidase is in the normal intestinal bacteria (in the colon) and can break down oligosaccharides, but it is not in the intestinal mucosa.

These carbohydrates escape digestion because there is no α - galactosidase activity in mammalian intestinal mucosa and because they are not absorbed into the blood.

Consequently, bacteria in the lower intestinal tract metabolize them to form large amounts of carbon dioxide and hydrogen and to lower the pH. The amount and pattern of expelled gases reflect differences in type, location, and abundance of intestinal microorganisms possessing α - galactosidase activity in a favorable nutrient environment.

Comparative effects of exogenous lactase (beta - galactosidase) preparations on in vivo lactose digestion.

Corazza GR, Benati G, Sorge M, et al. beta - Galactosidase from Aspergillus niger in adult lactose malabsorption: a double - blind crossover study.

In humans, alpha - galactosidase is normally found within lysosomes, but not expressed in the gastrointestinal tract.In humans, alpha - galactosidase is normally found within lysosomes, but not expressed in the gastrointestinal tract.in the gastrointestinal tract.??

α - Galactosidase, found in the product Beano, can be used to digest the galactosyl units from these oligosaccharides, leaving sucrose for further digestion.