Sentences with phrase «galactosidase staining»
Senescence - associated β - galactosidase staining.
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(6) Moreover, subcutaneous adipose of prematurely - aged transgenic mice exhibited high levels of staining for the senescence marker senescence - associated - β - galactosidase (SAβ - gal) and expressed high levels of several established markers of senescence, including p21, p19, interleukin - 6, (insulin - like growth factor binding protein - 2 (Igfbp2), and Pai - 1; primary BubR1H / H; INK - ATTAC mouse embryonic fibroblasts forced artificially into senescence by oncogenic Ras or serial passage exhibited a subpopulation that was both GFP + and stained positively SAβ - gal.
In the picture depicted, one of the two copies of the gene of the mouse was replaced with the lac Z cassette expressing the bacterial beta galactosidase and its activity in the kidney is followed by staining.
Selection for expression of the gene requires transcription from a cellular promoter, and consequently a mutation in a cellular gene, and the activity of the tagged gene can be followed by staining for beta galactosidase activity.