Sentences with phrase «gene circuits»

We first verified that the sensing gene circuit, assembled from bacteria genes, was fully expressed in later plant generations.

An engineered gene circuit that coordinates bacteria via signaling chemicals (green circles) makes them die off when the culture gets too crowded.

Our previously described synthetic de-greening gene circuit shows a threshold - like response through generation of reactive oxygen species and photosystem disruption [7].

According to Lu, this work advances the quantitative understanding of gene circuit behaviors, and facilitates the transformation of gene network design from trial - and - error construction to rational forward engineering.

«Gene circuit design strategy to advance synthetic biology.»

Collectively, these results indicate that our synthetic sensing gene circuit shows ligand - dependent gene expression in plants.

Just as engineers rearrange capacitors and resistors to create different electric circuits, researchers can arrange gene promoters and repressors — stretches of DNA that control gene activity — to create gene circuits with different properties.

Lu and his graduate students, Chen Liao and Andrew Blanchard, recently addressed the challenge by constructing an integrated modeling framework for quantitatively describing and predicting gene circuit behaviors.

James Collins, a professor of biomedical engineering at Boston University who was not associated with this research, says, «Efforts in synthetic biology to create complex gene circuits are often hindered by unanticipated or uncharacterized interactions between submodules of the circuits.

Typical models regard gene circuits as isolated entities that do not interact with their hosts and focus only on the biochemical processes within the circuits, noted Lu.

«Gene circuit switches on inside cancer cells, triggers immune attack: Advance may open new pathways for cancer immunotherapy.»

Now researchers have rewired one such gene circuit to control the size of bacterial populations.

Plants containing the same gene circuit but with the phospho - accepting Asp53 mutated (PhoBD53A - GFP) did not show ligand - dependent nuclear translocation (Figure 2C), indicating that the phospho - relay is required for ligand - mediated nuclear translocation of PhoB in planta.

Because the response from the de-greening gene circuit involves threshold - like behavior, accuracy at lower levels of the ligand is currently more difficult to follow.

As she explains in for CNN, the two types of stem cells begin to «talk to each other» and «respond by turning on particular developmental gene circuits or by physically changing shape to accomplish some architectural remodeling.»

Among his recent work at the Wyss is development of a technology for synthesizing whole genes, and potentially whole gene circuits, that is faster, more accurate, and significantly less expensive than current methods.

We previously documented that induction of the de-greening gene circuit produces a quantitative decrease in Fv / Fm [7].

In contrast, transgenic plants containing the complete gene circuit show significant induction of GUS in the presence of the TNT ligand.

Likewise, the ability to tune gene circuits [33], [34], [35] may allow our detector plant system to be adjusted as applications demand (e.g., rapid response for transportation hubs or better reset for environmental monitoring).

This de-greening gene circuit provides a visual response or readout that is readily recognized, remotely detectable, relatively rapid (less than two hours), and resettable [7].

Reverse transcriptase - polymerase chain reaction (RT - PCT) analysis of synthetic sensing and signaling components confirms expression of components of the sensing gene circuit.

In previous studies, gene circuits with predefined behaviors have been successfully built and modeled, but mostly on a case - by - case basis.

Analogous to integrated circuits that underlie myriad electronic products, engineered gene circuits can be used to generate defined dynamics, rewire endogenous networks, sense environmental stimuli, and produce valuable biomolecules.

Changes induced with the de-greening gene circuit can also be followed with hyperspectral imaging, which allows remote sensing and application of automated recognition by computers [24].

«We have performed a very innovative and ambitious study: we applied a three - step approach for the effective exploration and creation of successful synthetic gene circuits.

Over the last 17 years, scientists and engineers have developed synthetic gene circuits that can program the functionality, performance, and behavior of living cells.

These gene circuits hold great promise in medical and biotechnological applications, such as combating super bugs, producing advanced biofuels, and manufacturing functional materials.

Researchers have turned to quantitative modeling to address this gene circuit design challenge.

Researchers at MIT have developed a synthetic gene circuit that triggers the body's immune system to attack cancers when it detects signs of the disease.

To do this, Lu and a team including MIT postdocs Lior Nissim and Ming - Ru Wu, have built a gene circuit encoded in DNA designed to distinguish cancer cells from noncancer cells.

(D) Real - time qRT - PCR confirming that plants responding as shown in A, B, and C results from activation of the de-greening gene circuit.

These results indicate that the TNT ligand causes induction of the de-greening gene circuit and the response is not caused by stress, as expression of POR is known to increase in response to stress [7], [25], [26].

To confirm that the visual and quantitative responses are a consequence of ligand dependent induction of the de-greening gene circuit we used quantitative RT - PCR.

Response of transgenic tobacco plants with one copy of each gene circuit, de-greening gene circuit and synthetic sensing gene circuit.

We used quantitative RT - PCR to confirm and measure activation of the de-greening gene circuit (Figure 4D — E).

The sensing gene circuit is contained on a T - DNA providing resistance to Basta whereas the de-greening gene circuit is on a T - DNA providing resistance to kanamycin.

Transgenic plant lines with one copy of each gene circuit were exposed to 100 nM TNT in a setup identical to that of NT4.

Lines with one copy of each gene circuit consistently respond to TNT (Figure S6), though the response is not as strong.

Hence, we linked output of our complete synthetic sensing gene circuit to the de-greening gene circuit by placing its genes (diRNA POR, protochlorophyllide oxidoreductase; AtChlase, chlorophyllase; AtRCCR, red chlorophyll catabolite reductase)[7] under control of the PlantPho promoter (Figure 1).

Figure S4 shows expression analysis of all components of the sensing gene circuit (ss - TNT.

The sensing gene circuit (BastaR, ssTNT.R3 → Fls - Trg - PhoR → PhoB - VP64) and de-greening gene circuit (KanR, PlantPho:: de-greening genes) were introduced into Arabidopsis and tobacco plants and primary transformants generated.

We then tested the response of transgenic plants with the sensing and de-greening gene circuits to the TNT ligand using conditions that mimic real - world situations.

We have previously described a plant response system that produces white plants when a «de-greening gene circuit» is transcriptionally induced with an estrogen - like hormone [7].