Sentences with phrase «gene constructs»

The reporter gene construct pXP2 (− 310) was cotransfected with indicated expression plasmids into STAT1 - null U3A cells (C) or IRF - 9 — deficient U2A cells (D).

Even though the work was not hard for me — maintaining mouse strains, isolating DNA, PCR, primer design, preparing to make a new gene construct, northern blots — it took me a lot of time to translate so many English terms into Chinese in my mind.

Prior to transplantation, we analyzed the in vitro production of specific growth factors by unmodified hNPCctx and by hNPCctx transduced with a lentiviral gene construct designed to constitutively express GDNF (hNPCctx - GDNF)[11], [44].

To patent a novel gene construct used in this or that creature seems fair enough, as it is truly inventive.

Most notably, the studies are based on transient transfection of wild - type and mutant gene constructs into cultured cells or into zebrafish embryos and are unlikely to reproduce the specific mutant gene dose that is present in heterozygous mutant cells in FOP patients.

TEV - B plants were Agroinfected with Agrobacterium tumefaciens GV3101 containing the gene 5.91 scFv:: chloro gene construct.

Luciferase activity was shown relative to the values obtained in the cells transfected with the same reporter gene construct and empty vectors.

Immunocytochemical analysis revealed that 75.9 ± 11.8 % of the hNPCctx — GDNF population stably expressed the lentiviral gene construct.

«Recent studies found out that the Golden Rice have unintended effects when the gene constructs affected the plant's own genes which now affects the plant's growth,» Lopez said.

The researchers identified several reasons for this: The new gene constructs interfere with the plant's own gene for producing growth hormones, and the additional gene constructs were not, as intended, active solely in the kernels, but also in the leaves.

The cells were cotransfected with the reporter gene constructs and related expression plasmids as indicated by using SuperFect (Qiagen) followed by transcriptional activity assay using Dual - Luciferase Assay System (Promega) according to the manufacturer's instruction.

D, different reporter gene constructs were, respectively, cotransfected with IRF - 1 or IRF - 9 / STAT2 into U3A cells, as indicated.

C, schematic representation of the wt or mutant RIG - G promoter - luciferase reporter gene constructs.

Gene constructs were made by gene synthesis (GeneartAG) using sequences that were codon - optimized for expression in human cells.

Both gene constructs were inserted into a PVX pGR106 amplicon vector and amplified in E.coli.

Gene constructs of the newly made 5.91 - scFv were designed to target the chloroplast and ER regions of the tobacco leaf cells.