Sentences with phrase «gene copies using»

We quantified total 16S rRNA gene copies using quantitative PCR and found that placental samples and negative controls contained low and indistinguishable copy numbers.

Not exact matches

Gene therapy involves the delivery, using a modified virus, of a healthy copy of a gene to make up for one that's deficient in a way that causes diseGene therapy involves the delivery, using a modified virus, of a healthy copy of a gene to make up for one that's deficient in a way that causes disegene to make up for one that's deficient in a way that causes disease.

Lacking money to outfit the new lab, Wang and Xin bought used equipment: a single channel DNA sequencer (bought and barely touched by a Harvard Medical School researcher); second - hand centrifuges and pipettes; a reconditioned CytoScan HD system that detects variations in the number of copies of a gene.

The scientists are also experimenting with gene therapy, using a harmless virus to deliver a normal copy of the normal CIB2 gene to baby mice that have the mutated version.

Dr. Satish Rattan, Professor in the Department of Medicine, Division of Gastroenterology and Hepatology at Jefferson's Sidney Kimmel Medical College, together with Drs. Jagmohan Singh and Ipsita Mohanty, used altered copies of the body's own genetic make - up — small RNA fragments (microRNAs) that regulate the target gene RhoA / ROCK — in order to strengthen or weaken the muscle tone of the sphincter.

Everyone was used to thinking that we get one copy of each gene from our mother, one from our father.

In a new study published in The Quarterly Review of Biology, Dr. Karen Hardy and her team bring together archaeological, anthropological, genetic, physiological and anatomical data to argue that carbohydrate consumption, particularly in the form of starch, was critical for the accelerated expansion of the human brain over the last million years, and coevolved both with copy number variation of the salivary amylase genes and controlled fire use for cooking.

Ovarian cancer cells use autophagy all of the time, but also lose several copies of autophagy genes resulting in a compromised capacity.

For example, the genes the microbe uses to copy genetic information from DNA and translate it into proteins are very similar to the ones we use.

Instead, embryos used the mother's healthy copy of the gene to repair the cut.

The gene is silenced because it is on the part of their chromosome they inherited from their mother, and for mysterious reasons our cells use the father's copy of this gene.

Messenger RNA is a small piece of genetic material that the DNA uses to sythesize proteins, a sort of carbon copy of part of the information contained in the gene.

In the latest experiments the Mitalipov group focused on snipping out the mutated gene in heterozygous cells — a situation in which there was still a «good» nonmutated copy available for the natural cellular repair systems in the embryo to use as a template for repair after the researchers edited out the problematic one.

The researchers used the polymerase chain reaction to copy pieces of DNA from nearly all of M. tuberculosis» 4000 genes and dabbed an array of them onto glass slides.

His team plans to introduce a healthy copy of the CF gene into cells lining the nose and lungs, using a lipid suspension that fuses with the cell membrane and allows the gene to enter the cell.

Mitochondrial fingerprinting is normally used when the genetic material in a sample is degraded, for while there are only two copies of each nuclear gene in a cell, there are often hundreds of copies of mitochondrial DNA (mtDNA).

Researchers used a technique called CRISPR / Cas9 - mediated genome editing, which can precisely remove a mutation in DNA, allowing the body's DNA repair mechanisms to replace it with a normal copy of the gene.

Gene therapy can be used to neutralise harmful human genes, such as those that cause cancer, or to insert healthy copies of damaged genes into people with a genetic disease.

In research published in the Journal of Bone and Mineral Research, Javed, a professor in the UAB School of Dentistry's Department of Oral and Maxillofacial Surgery, used a mouse model with mutations in both copies of the gene for Sp7.

While the increase in EPSPS gene copies has created a nonsustainable way of controlling kochia with glyphosate - only programs, Jugulam said this resistance was evolved as the result of continuous use of glyphosate and the lack of herbicide diversity in controlling this weed.

The cutting edge, however, is «gene doping,» in which additional copies of a naturally - occurring gene or modified copies may be inserted into an athlete's genome using a virus carrier.

Newly developed approaches that use CRISPR / Cas9 gene editing technology can generate offspring that carry copies of the altered gene on both chromosomes — a phenomenon called super-Mendelian inheritance that, in theory, should quickly convert an entire population.

The researchers used an experimental technique — «droplet digital polymerase chain reaction» — to determine the number of copies of the genes that appear in these series.

Then De Luca and colleagues used a retrovirus to insert a healthy copy of the LAMB3 gene into DNA in the lab - grown skin stem cells.

Using clinical, genetic, and gene expression data as filters to distinguish genes whose copy number alteration causes cancer from those for whom copy number changes are incidental, the team whittled down their list from 14,000 to a more manageable number, each of which they systematically tested using genetic experiments in aniUsing clinical, genetic, and gene expression data as filters to distinguish genes whose copy number alteration causes cancer from those for whom copy number changes are incidental, the team whittled down their list from 14,000 to a more manageable number, each of which they systematically tested using genetic experiments in aniusing genetic experiments in animals.

The technique replaces the defective gene response for cystic fibrosis by using inhaled molecules of DNA to deliver a normal working copy of the gene to lung cells.

Gene therapy uses a virus to insert a functioning copy of a gene into defective ceGene therapy uses a virus to insert a functioning copy of a gene into defective cegene into defective cells.

Using cells from Down patients, scientists at the University of Massachusetts Medical School transplanted the XIST gene into that third copy.

Using postmortem human brain samples, the researchers found that variations in the number of copies of the C4 gene that people had, and the length of their gene, could predict how active the gene was in the brain.

For more than two decades, researchers have explored using gene therapy, an experimental treatment, to replace the flawed gene with a healthy copy.

To further explore nuclear transport's role in Huntington's disease, Grima took lab - grown mouse neurons and used chemical switches to a) turn on both an additional healthy copy of the RanGAP1 gene and a mutant version of Huntingtin; b) just turn on the mutant Huntingtin; or c) just turn on a healthy version of Huntingtin.

In the new study, Deisseroth's team used mice that lack both copies of CNTNAP2, a gene linked to autism.

In this study, researchers assessed the outcomes of 280 prostate cancer (Cap) patients, and reviewed the DNA «fingerprints» of each patient's tumor (using the patient's initial diagnostic core biopsy) to determine if gene copy number alterations (CNAs), or breaks in CFSs, were related to a less positive response to treatment.

In the study, which began in 2011, an adeno - associated virus vector was used to carry a functional copy of the affected gene to muscle cells in the diaphragms of nine patients.

Bacteria use small circuits of genes to manage routine tasks like copying their DNA or neutralizing a toxin.

Anahuac lacks a functional copy of the Rwt3 gene, which wheat uses to defend itself against the fungus, Yoshihiro Inoue of Kobe University in Japan and colleagues discovered.

Some research teams over the past five years have used microarray or gene - chip technology to compare genomes and quickly scan them for variations of copy numbers on each chromosome.

But before it could be used in people, researchers will need to achieve reprogramming without leaving behind copies of the genes involved, which can trigger cancer.

The researchers found that people who have more copies of the gene variants (by inheriting a copy of each advantageous variant from both parents), had the least amount of oxygen in their blood and used it more efficiently than people without either variant, or only one copy from one parent.

They also gave the cells a correct copy of the A1At gene so that the cell's DNA repair machinery would use it as a template to heal the break.

This receptor is made using one of two duplicated copies of the T - cell receptor gene, called C1 or C2, at random.

This copying is the first step in using the gene's «blueprint» to manufacture a needed protein.

By inserting the gene for a new trait alongside genes for a DNA - cutting enzyme and an RNA guide, scientists can prompt a cell to slice out copies of the original, wild - type gene from its chromosomes and use the inserted gene as a template for repair.

They thus need to be temporarily displaced to allow genes to be copied («transcribed») into the messages that are used to make proteins.

the chemical, similar to DNA, that makes up the «message» molecules that cells use as working copies of genes, when manufacturing proteins.

Using DECoN (which stands for Detection of Exon Copy Number variants), the researchers took advantage of the high density of sequencing data available in new gene panels to accurately detect deletions or duplications of exons.

In the new study, a team led by Harvard University's George Church — one of the pioneers of the CRISPR technique — used gene editing to remove all the copies of porcine endogenous retroviruses (or PERVs) from their pig cells» DNA.

From these data, we first measured allele - specific expression, where a heterozygous site in a gene can be used to distinguish gene expression from the two copies of a gene.

Using this technology, we screened 31 patient samples across an array containing a total of 162 exons for five disease genes and detected copy - number changes, ranging from whole - gene deletions and duplications to single - exon deletions and duplications, in 100 % of the cases.

Over the next few years genes from different bacterial (and eventually mammalian) species were cloned into plasmids and new cloning vectors such as pBR322, pACYC, and pUC were developed to provide higher copy number vectors that could be used in these cloning experiments.